High accuracy mass measurement of peptides with internal calibration using a dual electrospray ionization sprayer system for protein identification

2002 ◽  
Vol 16 (6) ◽  
pp. 505-511 ◽  
Author(s):  
Feng Zhou ◽  
Wenqing Shui ◽  
Yu Lu ◽  
Pengyuan Yang ◽  
Yinlong Guo
Keyword(s):  
Electrospray Ionization ◽  
Protein Identification ◽  
Mass Measurement ◽  
High Accuracy ◽  
Internal Calibration ◽  
Dual Electrospray Ionization

scholarly journals Research on the Influence of Air Pressure to High Accuracy Mass Measurement

2017 ◽  
Author(s):  
Chang-Qing Cai ◽  
Man-Hong Hu ◽  
Jian Wang ◽  
Yue Zhang ◽  
Tao Huang ◽  
...  
Keyword(s):  
Mass Measurement ◽  
High Accuracy ◽  
Air Pressure

scholarly journals Determination of ramipril in human plasma and its fragmentation by UPLC-Q-TOF-MS with positive electrospray ionization

2015 ◽  
Vol 65 (2) ◽  
pp. 159-169 ◽  
Author(s):  
Paweł Szpot ◽  
Grzegorz Buszewicz
Keyword(s):  
Electrospray Ionization ◽  
Human Plasma ◽  
Ultra Performance Liquid Chromatography ◽  
High Accuracy ◽  
Coefficient Of Determination ◽  
Limit Of Quantification ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Chemical Formulas

Abstract This report presents the application of ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry with positive electrospray ionization, to determine ramipril in human plasma. First, the proteins in human plasma were precipitated using acetonitrile, then the supernatant was extracted by ethyl acetate at pH 3 and finally, the extract was analyzed using a UPLC-QTOF- MS system. The method was validated and the coefficient of determination (R2) was > 0.999, the lower limit of quantification (LLOQ) was 0.5 ng mL-1. Precision, recovery and stability were determined for three different concentrations of ramipril. RSD for this method ranged from 3.3 to 8.6 %. The intra-day mean recovery was from 65.3 to 97.3 %. In addition, the fragmentation of ramipril was studied. Due to high resolution of the spectrometer, it was possible to measure fragment masses accurately and determine their molecular and chemical formulas with high accuracy.

Improved protein identification using automated high mass measurement accuracy MALDI FT-ICR MS peptide mass fingerprinting

2004 ◽  
Vol 238 (2) ◽  
pp. 189-196 ◽  
Author(s):  
David M. Horn ◽  
Eric C. Peters ◽  
Heath Klock ◽  
Andrew Meyers ◽  
Ansgar Brock
Keyword(s):  
Protein Identification ◽  
Measurement Accuracy ◽  
Mass Measurement ◽  
Peptide Mass Fingerprinting ◽  
High Mass ◽  
Mass Measurement Accuracy ◽  
Peptide Mass ◽  
Ft Icr Ms ◽  
Ft Icr

Induced Dual-Nanospray: A Novel Internal Calibration Method for Convenient and Accurate Mass Measurement

2013 ◽  
Vol 24 (9) ◽  
pp. 1446-1449 ◽  
Author(s):  
Yafeng Li ◽  
Ning Zhang ◽  
Yueming Zhou ◽  
Jianing Wang ◽  
Yiming Zhang ◽  
...  
Keyword(s):  
Mass Measurement ◽  
Calibration Method ◽  
Accurate Mass Measurement ◽  
Accurate Mass ◽  
Internal Calibration
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