The research aimed to investigate the ecotoxicity of nano Ni(OH)2 from the leakage of lithium battery leakage on Carassius auratus. Choosing Carassius auratus as test biology, Researched on Content of free radical in the liver, SOD, GST and GSH. The main findings are as follows: As proved by EPR spinning trapping, nano Ni(OH)2 may induce the generation of hydroxyl radical in the body of Carassius auratus, which may impose significant impact on such active oxygen scavenging systems as SOD, GST and GSH. Nano Ni(OH)2 as concentrated in the fish body may incur redox reaction, and produce redox metabolic intermediate. The study provides the theoretical basis for safety problems might be incurred by mutual action between nano-material and organisms. As one of important toxic mechanisms of nano Ni(OH)2 to Carassius auratus, oxidative stress mechanism requires further study.