scholarly journals A Cascaded Droplet Microfluidic Platform Enables High‐Throughput Single Cell Antibiotic Susceptibility Testing at Scale

Small Methods ◽  
2021 ◽  
pp. 2101254
Author(s):  
Pengfei Zhang ◽  
Aniruddha M. Kaushik ◽  
Kuangwen Hsieh ◽  
Sixuan Li ◽  
Shawna Lewis ◽  
...  
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Antibiotic Susceptibility Testing ◽  
Microfluidic Platform

Diffusion–Convection Hybrid Microfluidic Platform for Rapid Antibiotic Susceptibility Testing

2021 ◽  
Author(s):  
Ann V. Nguyen ◽  
Morteza Azizi ◽  
Mohammad Yaghoobi ◽  
Belgin Dogan ◽  
Shiying Zhang ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Antibiotic Susceptibility Testing ◽  
Microfluidic Platform ◽  
Diffusion Convection

scholarly journals A microfluidic platform for rapid, stress-induced antibiotic susceptibility testing of Staphylococcus aureus

Lab on a Chip ◽  
2012 ◽  
Vol 12 (21) ◽  
pp. 4523 ◽  
Author(s):  
Maxim Kalashnikov ◽  
Jean C. Lee ◽  
Jennifer Campbell ◽  
Andre Sharon ◽  
Alexis F. Sauer-Budge
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Antibiotic Susceptibility Testing ◽  
Microfluidic Platform

scholarly journals A portable and high-integrated 3D microfluidic chip for bacterial quantification and antibiotic susceptibility testing

2021 ◽  
Author(s):  
Wenshuai Wu ◽  
Gaozhe Cai ◽  
Yang Liu
Keyword(s):  
Single Cell ◽  
Antibiotic Susceptibility ◽  
Bacterial Infections ◽  
Susceptibility Testing ◽  
Dynamic Range ◽  
Antibiotic Susceptibility Testing ◽  
Technical Potential ◽  
Uniform Sample ◽  
Pdms Chip ◽  
User Friendly

On-site single-cell antibiotic susceptibility testing (sc-AST) provides unprecedented technical potential to improve the treatment of bacterial infections and study heterogeneous resistance to antibiotics. Herein, we developed a portable and high-integrated 3D polydimethylsiloxane (PDMS) chip to perform fast and on-site bacteria quantification and sc-AST. The 3D arrangement of the chambers significantly improved the integration of reaction units (~10000/cm2) and widened the dynamic range to 5 orders of magnitude. A capillary valve-based flow distributor was adopted for flow equidistribution in 64 parallel channels and uniform sample loading in as short as 2 s. The degassed PDMS enabled this device to independently dispense the sample into 3D chamber array with almost 100% efficiency. The quantification of Escherichia coli (E. coli) strains with various activity was accomplished in 0.5-2 h, shortened by 20 h in comparison to the traditional plate counting. The functionality of our platform was demonstrated with several effective antibiotics by determining minimum inhibitory concentrations at single-cell level. Furthermore, we utilized the lyophilization of test reagents and needle-mediated reagents rehydration to realize one-step on-site sc-AST. The results indicate that the proposed sc-AST platform is portable, highly sensitive, fast, accurate and user-friendly, thus it has the potential to facilitate precise therapy in time and monitor the treatment. Meanwhile, it could serve as an approach for investigating the mechanisms of heteroresistance at single-cell resolution.

Rapid antibiotic susceptibility testing by tracking single cell growth in a microfluidic agarose channel system

Lab on a Chip ◽  
2013 ◽  
Vol 13 (2) ◽  
pp. 280-287 ◽  
Author(s):  
Jungil Choi ◽  
Yong-Gyun Jung ◽  
Jeewoo Kim ◽  
Sungbum Kim ◽  
Yushin Jung ◽  
...  
Keyword(s):  
Cell Growth ◽  
Single Cell ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Antibiotic Susceptibility Testing ◽  
Channel System

Nanoliter-Sized Microchamber/Microarray Microfluidic Platform for Antibiotic Susceptibility Testing

2018 ◽  
Vol 90 (24) ◽  
pp. 14137-14144 ◽  
Author(s):  
Morteza Azizi ◽  
Meisam Zaferani ◽  
Belgin Dogan ◽  
Shiying Zhang ◽  
Kenneth W. Simpson ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Antibiotic Susceptibility Testing ◽  
Microfluidic Platform

A multiplexed microfluidic platform for rapid antibiotic susceptibility testing

2013 ◽  
Vol 49 ◽  
pp. 118-125 ◽  
Author(s):  
Ritika Mohan ◽  
Arnab Mukherjee ◽  
Selami E. Sevgen ◽  
Chotitath Sanpitakseree ◽  
Jaebum Lee ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Antibiotic Susceptibility Testing ◽  
Microfluidic Platform

Bacterial classification and antibiotic susceptibility testing on an integrated microfluidic platform

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Alexandros A. Sklavounos ◽  
Carine R. Nemr ◽  
Shana O. Kelley ◽  
Aaron R. Wheeler
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Microfluidic System ◽  
Antibiotic Susceptibility Testing ◽  
Metabolic Markers ◽  
Microfluidic Platform ◽  
Bacterial Classification ◽  
Digital Microfluidic

An ‘all in one’ digital microfluidic system is reported that automates the preparation and testing of samples for antibiotic susceptibility and bacterial classification, relying on three metabolic markers.

scholarly journals Whole Slide Imaging for High-Throughput Sensing Antibiotic Resistance at Single-Bacterium Level and Its Application to Rapid Antibiotic Susceptibility Testing

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2441 ◽  
Author(s):  
Donghui Song ◽  
Haomin Liu ◽  
Huayi Ji ◽  
Yu Lei
Keyword(s):  
Antibiotic Resistance ◽  
High Throughput ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Large Population ◽  
Accurate Determination ◽  
Great Promise ◽  
Antibiotic Susceptibility Testing ◽  
Whole Slide Imaging ◽  
Antibiotic Resistant

Since conventional culture-based antibiotic susceptibility testing (AST) methods are too time-consuming (typically 24–72 h), rapid AST is urgently needed for preventing the increasing emergence and spread of antibiotic resistant infections. Although several phenotypic antibiotic resistance sensing modalities are able to reduce the AST time to a few hours or less, concerning the biological heterogeneity, their accuracy or limit of detection are limited by low throughput. Here, we present a rapid AST method based on whole slide imaging (WSI)-enabled high-throughput sensing antibiotic resistance at single-bacterium level. The time for determining the minimum inhibitory concentration (MIC) was theoretically shortest, which ensures that the growth of each individual cell present in a large population is inhibited. As a demonstration, our technique was able to sense the growth of at least several thousand bacteria at single-cell level. Reliable MIC of Enterobacter cloacae against gentamicin was obtained within 1 h, while the gold standard broth dilution method required at least 16 h for the same result. In addition, the application of our method prevails over other imaging-based AST approaches in allowing rapid and accurate determination of antibiotic susceptibility for phenotypically heterogeneous samples, in which the number of antibiotic resistant cells was negligible compared to that of the susceptible cells. Hence, our method shows great promise for both rapid AST determination and point-of-care testing of complex clinical bacteria isolates.

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