Brief Report: Dclk1 Deletion in Tuft Cells Results in Impaired Epithelial Repair After Radiation Injury

Intestinal epithelial damage is associated with most digestive diseases and results in detrimental effects on nutrient absorption and production of hormones and antimicrobial defense molecules. Thus, understanding epithelial repair and regeneration following damage is essential in developing therapeutics that assist in rapid healing and restoration of normal intestinal function. Here we used a well-characterized enteric virus (rotavirus) that damages the epithelium at the villus tip but does not directly damage the intestinal stem cell, to explore the regenerative transcriptional response of the intestinal epithelium at the single-cell level. We found that there are specific Lgr5+ cell subsets that exhibit increased cycling frequency associated with significant expansion of the epithelial crypt. This was accompanied by an increase in the number of immature enterocytes. Unexpectedly, we found rotavirus infects tuft cells. Transcriptional profiling indicates tuft cells respond to viral infection through interferon-related pathways. Together these data provide insights as to how the intestinal epithelium responds to insults by providing evidence of stimulation of a repair program driven by stem cells with involvement of tuft cells that results in the production of immature enterocytes that repair the damaged epithelium.

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EP2 receptor null mice have an impaired intestinal response to radiation injury

Gastroenterology ◽

10.1016/s0016-5085(01)80049-4 ◽

2001 ◽

Vol 120 (5) ◽

pp. A10-A10

Author(s):

C HOUCHEN ◽

B DIECKGRAEFE ◽

M STUMOSKI ◽

W STENSON

Keyword(s):

Radiation Injury ◽

Ep2 Receptor

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Radiation Injury to the Small Bowel with Special Consideration of Surgical Complications

Gastroenterology ◽

10.1016/s0016-5085(19)35907-4 ◽

1956 ◽

Vol 31 (1) ◽

pp. 47-55 ◽

Cited By ~ 14

Author(s):

H. Harvey Peterson ◽

Edwin G. Clausen

Keyword(s):

Small Bowel ◽

Surgical Complications ◽

Radiation Injury ◽

Special Consideration

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Outcomes of surgery for resection of regions of symptomatic radiation injury after stereotactic radiosurgery for arteriovenous malformations

Yearbook of Neurology and Neurosurgery ◽

10.1016/s0513-5117(08)79134-8 ◽

2008 ◽

Vol 2008 ◽

pp. 211-212

Author(s):

G.J. Zipfel

Keyword(s):

Stereotactic Radiosurgery ◽

Arteriovenous Malformations ◽

Radiation Injury

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Radiation Sickness after Therapeutic Administration of 806 mCi of 131I for Thyroid Cancer

Nuklearmedizin ◽

10.1055/s-0037-1621327 ◽

1967 ◽

Vol 06 (02) ◽

pp. 170-183

Author(s):

K. Šilink ◽

J. Němec ◽

J. Kubal ◽

S. Röhling ◽

S. Vohnout

Keyword(s):

Bone Marrow ◽

Thyroid Cancer ◽

Radiation Injury ◽

Clinical Course ◽

External Irradiation ◽

Bone Marrow Depression ◽

Erythroid Hyperplasia ◽

Cytological Features ◽

Haematological Changes ◽

Therapeutic Administration

SummaryThe clinical course and the haematologic events in a patient suffering from metastatic thyroid cancer after administration of 806 mCi of 131I are described. A serious bone marrow depression developed and was treated successfully. The haematological changes during the early and late phases of the radiation injury were studied in detail and compared with those after external irradiation. The haematological events after internal irradiation with 131I are characterised by initial neutrophilic leukocytosis, protracted lymphopenia, slowly developing anaemia reaching lowest values about 3 months after administration, erythroid hyperplasia in the bone marrow after recovery from bone marrow depression and prominent cytological features of the bone marrow, especially pronounced erythropoietic polyploidy.

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