scholarly journals Effects on Proliferation and Differentiation of Multipotent Bone Marrow Stromal Cells Engineered to Express Growth Factors for Combined Cell and Gene Therapy

Stem Cells ◽  
2011 ◽  
Vol 29 (11) ◽  
pp. 1727-1737 ◽  
Author(s):  
Fernando A. Fierro ◽  
Stefanos Kalomoiris ◽  
Claus S. Sondergaard ◽  
Jan A. Nolta
Keyword(s):  
Gene Therapy ◽  
Bone Marrow ◽  
Growth Factors ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Proliferation And Differentiation ◽  
Cell And Gene Therapy

scholarly journals 1,25-Dihydroxyvitamin D3 stimulates the production of insulin-like growth factor-binding proteins-2, -3 and -4 in human bone marrow stromal cells

2001 ◽  
pp. 549-557 ◽  
Author(s):  
M Kveiborg ◽  
A Flyvbjerg ◽  
EF Eriksen ◽  
M Kassem
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Binding Proteins ◽  
Marrow Stromal Cells ◽  
Mrna Levels ◽  
Dihydroxyvitamin D3 ◽  
Igf System ◽  
Proliferation And Differentiation ◽  
Igfbp 3

BACKGROUND: 1,25-Dihydroxyvitamin D3 (calcitriol) inhibits proliferation and stimulates differentiation of multiple cell types, including osteoblasts. Human (h) bone marrow stromal cells (MSCs) are a homogenous non-hematopoietic population of cells present in the bone marrow and exhibit a less differentiated osteoblastic phenotype. The IGF system, including IGFs-I, and -II and IGF binding proteins (IGFBPs), plays an important role in osteoblast cell proliferation and differentiation. OBJECTIVE: To examine the pattern of expression of the IGF system in hMSCss and its regulation by calcitriol. METHODS AND RESULTS: hMSCs express mRNA of both IGFs-I, and -II and IGFBPs-1 to -6 as shown by RT-PCR and northern blot analysis. As assessed by western ligand blotting (WLB) and western immmunoblot analysis, hMSCs secrete 38-42 kDa IGFBP-3, 24-28 kDa IGFBP-4 and a 33 kDa IGFBP-2. Calcitriol (dose range 10-10 mol/l) exerted no consistent dose-dependent effects on either IGF-I or IGF-II mRNA levels. In contrast, calcitriol treatment increased steady-state mRNA levels of IGFBPs-2, -3 and -4, but had no effect on IGFBP-5 or -6. Similarly, calcitriol increased the secretion of IGFBPs-2, -3 and -4 as determined by WLB. We found no detectable basal IGFBP-3 or IGFBP-4 protease activities in the absence or presence of calcitriol treatment. CONCLUSIONS: Our results demonstrate that hMSCs expressed a distinct pattern of IGFs and IGFBPs that may be related to their stage of differentiation. The observed increase in production of IGFBPs-2, -3 and -4 by hMSCs upon treatment with calcitriol may be an important mechanism mediating the effects of calcitriol on MSC proliferation and differentiation.

Localex vivo gene therapy with bone marrow stromal cells expressing human BMP4 promotes endosteal bone formation in mice

2004 ◽  
Vol 6 (1) ◽  
pp. 4-15 ◽  
Author(s):  
Xiao S. Zhang ◽  
Thomas A. Linkhart ◽  
Shin-Tai Chen ◽  
Hairong Peng ◽  
Jon E. Wergedal ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Bone Marrow ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells

scholarly journals Influence of an Autologous Serum-Supplemented Medium on the Proliferation and Differentiation into Neurons of Canine Bone Marrow Stromal Cells

2012 ◽  
Vol 74 (6) ◽  
pp. 817-820 ◽  
Author(s):  
Kazuya EDAMURA ◽  
Takashi KUROSAWA ◽  
Rei NAKANO ◽  
Kenji TESHIMA ◽  
Kazushi ASANO ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Autologous Serum ◽  
Proliferation And Differentiation

Enhanced proliferation and differentiation of rat hepatocytes cultured with bone marrow stromal cells

2001 ◽  
Vol 189 (1) ◽  
pp. 106-119 ◽  
Author(s):  
Toru Mizuguchi ◽  
Thomas Hui ◽  
Kaia Palm ◽  
Nozomu Sugiyama ◽  
Toshihiro Mitaka ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Rat Hepatocytes ◽  
Marrow Stromal Cells ◽  
Proliferation And Differentiation
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