scholarly journals Usage of meat and bone meal in animal, poultry and fish feeds: A survey and risk analysis for the occurrence of bovine spongiform encephalopathy in Bangladesh

2021 ◽  
Author(s):  
Md. Nazrul Islam ◽  
Md. Saiful Islam Siddiqui ◽  
Md. Taohidul Islam ◽  
Md. Rafiqul Islam ◽  
Emdadul Haque Chowdhury
Keyword(s):  
Risk Analysis ◽  
Bovine Spongiform Encephalopathy ◽  
Spongiform Encephalopathy ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Fish Feeds

Real-Time PCR Detection of Ruminant DNA

2004 ◽  
Vol 67 (3) ◽  
pp. 550-554 ◽  
Author(s):  
LUIS MENDOZA-ROMERO ◽  
EDWARD L. C. VERKAAR ◽  
PAUL H. SAVELKOUL ◽  
ARNOLD CATSBURG ◽  
HENK J. M. AARTS ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Bovine Spongiform Encephalopathy ◽  
Real Time ◽  
Real Time Pcr ◽  
Spongiform Encephalopathy ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Dna Targeting ◽  
Species Origin ◽  
Semiquantitative Method

To control the spread of bovine spongiform encephalopathy, several DNA methods have been described for the detection of the species origin of meat and bone meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semiquantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element Bov-A2. This method is specific for ruminants and is able to detect as little as 10 fg of bovine DNA. Autoclaving decreased the amount of detectable DNA, but positive signals were observed in feeding stuff containing 10% bovine material if this had not been rendered in accordance with the regulations, i.e., heated at 134°C for 3 instead of 20 min.

Are Bacterial Toxins Involved in the Aetiology of Transmissible Spongiform Encephalopathies?

1997 ◽  
Vol 11 (4) ◽  
pp. 301-307 ◽  
Author(s):  
T. Stockdale
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Bacterial Toxins ◽  
Transmissible Spongiform Encephalopathies ◽  
Spongiform Encephalopathy ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Spongiform Encephalopathies ◽  
Infected Meat ◽  
Disease Epidemics ◽  
The Brain

It is argued that the conditions for Bovine Spongiform Encephalopathy and Creutzfeld-Jacob disease epidemics necessitate, in addition to a diet that contains infected meat and bone meal, the presence of leaky membranes that enable bacterial toxins to circulate in the blood and enter the brain.

A quantitative assessment of the risk of exposure to bovine spongiform encephalopathy via meat-and-bone meal in Japan

2006 ◽  
Vol 75 (3-4) ◽  
pp. 221-238 ◽  
Author(s):  
Takehisa Yamamoto ◽  
Toshiyuki Tsutsui ◽  
Takashi Nonaka ◽  
Sota Kobayashi ◽  
Akiko Nishiguchi ◽  
...  
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Quantitative Assessment ◽  
Spongiform Encephalopathy ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Risk Of Exposure

Development of Primers for Detection of Meat and Bone Meal in Ruminant Feed and Identification of the Animal of Origin

2004 ◽  
Vol 67 (6) ◽  
pp. 1289-1292 ◽  
Author(s):  
T. KUSAMA ◽  
T. NOMURA ◽  
K. KADOWAKI
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Atp Synthase ◽  
Individual Species ◽  
Spongiform Encephalopathy ◽  
Target Sequence ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Cattle Feed ◽  
Important Prerequisite ◽  
Ruminant Feed

The safe use of cattle feed free from meat and bone meal is an important prerequisite to prevent further spread of bovine spongiform encephalopathy. We designed primers to detect very small amounts of meat and bone meal in ruminant feed. Mitochondrial subunit 8 of the ATP synthase gene was used as a target sequence. PCR-based assays revealed amplification of DNA from mammals, ruminants, and individual species using these primers. The method allowed detection of the presence of meat and bone meal in ruminant feed from 0.1 to 0.01%. Sensitivity and effectiveness of the method for detecting prohibited animal proteins in ruminant feed was evaluated.

scholarly journals Atypical H-Type Bovine Spongiform Encephalopathy in a Cow Born after the Reinforced Feed Ban on Meat-and-Bone Meal in Europe

2012 ◽  
Vol 50 (12) ◽  
pp. 4171-4174 ◽  
Author(s):  
C. Guldimann ◽  
M. Gsponer ◽  
C. Drogemuller ◽  
A. Oevermann ◽  
T. Seuberlich
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Spongiform Encephalopathy ◽  
Meat And Bone Meal ◽  
Bone Meal

scholarly journals The Microscopic Detection of Animal Proteins in Animal Feed Regarding Bovine Spongiform Encephalopathy

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Radmila Crceva Nikolovska ◽  
Aleksandra Angeleska ◽  
Srecko Gjorgjievski ◽  
Katerina Blagoevska ◽  
Riste Uzunov ◽  
...  
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Laying Hens ◽  
Animal Feed ◽  
Animal Protein ◽  
Spongiform Encephalopathy ◽  
Ruminant Nutrition ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Microscopic Method ◽  
Spongiform Encephalopathies

Abstract Due to the actuality of spongiform encephalopathies and their proven spreading by means of animal feed containing meat and bone meal, the description and measurement of osteocytic lacunae contributes to more easily distinguish bone fragments in meat and bone meal. Transmissible spongiform encephalopathies (TSEs) have attracted a lot of attention, especially after 1986, when the first case of BSE (bovine spongiform encephalopathy) was detected. Since the outbreak of spongiform encephalopathy (BSE), the use of animal protein including bone meal as an ingredient in animal feed has been controlled by several regulations including Regulation (EC) 999/2001, Regulation (EC) 1774/2002, and Regulation (EC) 1234/2003. The classical microscopic method is the only official method for detecting animal protein in animal feed in the European Union (Commission Regulation (EC) 152/2009). By applying the microscopic method to the animal feed samples, we performed detection in order to determine the presence of animal proteins that originate from mammals and fish. The microscopic analysis of all 421 samples, of which 115 were raw materials for the production of animal feed, 230 were concentrates for ruminant nutrition and 76 were concentrates for non-ruminant nutrition (32 concentrates for laying hens and 44 concentrates for pigs), did not provide positive results, that is, no remains of animal tissues of mammalian origin were found in any specimen. Whereas in 10 out of 32 (31.25%) concentrates intended for non-ruminant nutrition (laying hens), pieces of fish tissue were found. In these samples, we usually detected the presence of fish bones, gills and scales.

Detection and identification of animal-derived protein in feedingstuffs by enzyme-linked immunoassay

1991 ◽  
Vol 1991 ◽  
pp. 117-117
Author(s):  
P. A. Brett ◽  
P. R. Goodwin
Keyword(s):  
Great Britain ◽  
Bovine Spongiform Encephalopathy ◽  
Animal Species ◽  
Animal Protein ◽  
Spongiform Encephalopathy ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Animal Feeds ◽  
Regulatory Authorities ◽  
Detection And Identification

In Great Britain the use of animal-derived protein in animal feeds has recently been subject to restrictions (The Bovine Spongiform Encephalopathy Order 1988 and subsequent orders), including a prohibition on the use of animal protein produced from ruminant carcases in feed given to ruminants. More generally, livestock producers are increasingly demanding more detailed information about the ingredients included in the compound feeds they purchase, and may stipulate that meat and bone meal derived from particular animal species is not used in their feed. In order that compliance with these legal and commercial requirements can be monitored and enforced, it is important that feed manufacturers, their customers and the regulatory authorities should have recourse to a means of identifying the species from which animal protein, when present in a feed, is derived.

An epidemiologist’s view of bovine spongiform encephalopathy

1994 ◽  
Vol 343 (1306) ◽  
pp. 357-361 ◽  
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Spongiform Encephalopathy ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Original Source ◽  
Hydrocarbon Solvents ◽  
Epidemiological Features ◽  
Food Borne ◽  
Means Of Transmission ◽  
Sheep Scrapie

Bovine spongiform encephalopathy was first recognized in Great Britain in 1986 and was the result of infection with a scrapie-like agent surviving in m eat and bone meal used in feedstuffs. This effective exposure commenced in 1981-82 and was associated with a reduction in the use of hydrocarbon solvents in the manufacture of meat and bone meal. The epidemiological features are consistent with sheep scrapie as the original source, but the epidemic was amplified by the recycling of infected cattle tissue resulting in a marked increase in incidence from 1989. The food borne source was eliminated by legislation introduced in July 1988. The first effects of this became apparent during 1991 and these have become more obvious during 1993 with a reduction in the national incidence. Specific studies are still in progress to determine whether other means of transmission can occur, but none capable of maintaining the epidemic have been detected.

Utilization of Meat and Bone Meal Bottom Ash in Ceramics

1970 ◽  
Vol 17 (1) ◽  
pp. 86-92 ◽  
Author(s):  
Virginija VALANČIENĖ
Keyword(s):  
Compressive Strength ◽  
Ceramic Body ◽  
Bottom Ash ◽  
Porous Ceramics ◽  
Animal Waste ◽  
Spongiform Encephalopathy ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Firing Shrinkage ◽  
The Impact

During utilization of animal waste meat and bone meal (MBM) is received, realization and use of which has been stopped due to risk for the transmission of the bovine spongiform encephalopathy infection. The MBM must be safely stored or treated. Most often meat and bone meal undergoes thermal treatment. During combustion large quantities of residues (ashes) are received, the recycled use of which has been given a lot of attention lately. In this work it was investigated the impact of the additive of the bottom ash (BA) formed during combustion of the MBM on the properties of forming mass and ceramic body of hydromica clay, and also it was evaluated a possibility to use the MBM BA in manufacturing of building ceramics. After replacing the sand in porous ceramics by this additive the plasticity of the forming mass, drying and firing shrinkage as well as density of ceramic body changed insignificantly whereas the compressive strength increased by 8 % - 22 %. So the MBM BA can be utilized in production of porous ceramics.http://dx.doi.org/10.5755/j01.ms.17.1.256

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