Isolation and Characterization of the Cellobiose Dehydrogenase from the Brown-Rot Fungus Coniophora puteana (Schum ex Fr.) Karst.

Summary The decay patterns of brown and soft rot fungi in Sapelli wood (Entandrophragma cylindricum Sprague), with respect to natural durability, were examined by light and transmission electron microscopy as well as UV spectroscopy. Analyses revealed that the typical brown rot fungus Coniophora puteana can cause a soft rot-like decay in the sapwood of Sapelli at high moisture contents of approx. 100%. In accordance with the decay pattern of the typical soft rot fungus Chaetomium globosum, the hyphae of C. puteana penetrated the S2 wall of fibres and formed characteristic rhomboidal cavities orientated parallel to the microfibrillar angle. However, these cavities were larger in diameter (3.9 μm ± 0.9) than those formed by C. globosum (2.1 μm ± 0.5) and with a distinct interspace between the hyphae and surrounding walls. Hyphae of C. globosum were directly attached to the undecayed secondary wall. No lignin degradation for both fungi in close vicinity to the cavities was observed, as evidenced by UV spectroscopy. The inclusion of microscopy for definitive decay type identification is recommended.

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Characterization of a transplasma membrane redox system of the brown rot fungus Gloeophyllum trabeum

International Biodeterioration & Biodegradation ◽

10.1016/j.ibiod.2003.09.002 ◽

2004 ◽

Vol 53 (1) ◽

pp. 37-42 ◽

Cited By ~ 10

Author(s):

Weihong Qi ◽

Jody Jellison

Keyword(s):

Brown Rot ◽

Redox System ◽

Gloeophyllum Trabeum ◽

Brown Rot Fungus

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The genes encoding glycoside hydrolase family 6 and 7 cellulases from the brown-rot fungus Coniophora puteana

Journal of Wood Science ◽

10.1007/s10086-009-1042-4 ◽

2009 ◽

Vol 55 (5) ◽

pp. 376-380 ◽

Cited By ~ 7

Author(s):

Taira Kajisa ◽

Kiyohiko Igarashi ◽

Masahiro Samejima

Keyword(s):

Glycoside Hydrolase ◽

Brown Rot ◽

Glycoside Hydrolase Family ◽

Coniophora Puteana ◽

Brown Rot Fungus ◽

Genes Encoding ◽

Hydrolase Family

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Distribution, Characteristics, and Regulatory Potential of Long Noncoding RNAs in Brown-Rot Fungi

International Journal of Genomics ◽

10.1155/2019/9702342 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 2

Author(s):

Alessandra Borgognone ◽

Walter Sanseverino ◽

Riccardo Aiese Cigliano ◽

Raúl Castanera

Keyword(s):

Noncoding Rnas ◽

Gc Content ◽

Brown Rot ◽

Long Noncoding Rnas ◽

Upstream Gene ◽

Coniophora Puteana ◽

Brown Rot Fungi ◽

Serpula Lacrymans ◽

Genomic Regions

Long noncoding RNAs have been thoroughly studied in plants, animals, and yeasts, where they play important roles as regulators of transcription. Nevertheless, almost nothing is known about their presence and characteristics in filamentous fungi, especially in basidiomycetes. In the present study, we have carried out an exhaustive annotation and characterization of lncRNAs in two lignin degrader basidiomycetes, Coniophora puteana and Serpula lacrymans. We identified 2,712 putative lncRNAs in the former and 2,242 in the latter, mainly originating from intergenic locations of transposon-sparse genomic regions. The lncRNA length, GC content, expression levels, and stability of the secondary structure differ from coding transcripts but are similar in these two species and resemble that of other eukaryotes. Nevertheless, they lack sequence conservation. Also, we found that lncRNAs are transcriptionally regulated in the same proportion as genes when the fungus actively decomposes soil organic matter. Finally, up to 7% of the upstream gene regions of Coniophora puteana and Serpula lacrymans are transcribed and produce lncRNAs. The study of expression trends in these gene-lncRNA pairs uncovered groups with similar and opposite transcriptional profiles which may be the result of cis-transcriptional regulation.

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