cDNA Cloning of Brevinase, a Heterogeneous Two-Chain Fibrinolytic Enzyme from Agkistrodon blomhoffii brevicaudus Snake Venom, by Serial Hybridization–Polymerase Chain Reaction

2000 ◽  
Vol 377 (2) ◽  
pp. 234-240 ◽  
Author(s):  
Je-Wook Lee ◽  
Wan Park
Keyword(s):  
Polymerase Chain Reaction ◽  
Cdna Cloning ◽  
Snake Venom ◽  
Fibrinolytic Enzyme ◽  
Chain Reaction ◽  
Polymerase Chain

cDNA Cloning by Inverse Polymerase Chain Reaction

PCR Protocols ◽  
2003 ◽  
pp. 349-356 ◽  
Author(s):  
Sheng-He Huang ◽  
Chun-Hua Wu ◽  
Bing Cai ◽  
John Holcenberg
Keyword(s):  
Polymerase Chain Reaction ◽  
Cdna Cloning ◽  
Inverse Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

cDNA cloning of equine interleukin-2 by polymerase chain reaction

1993 ◽  
Vol 25 (3) ◽  
pp. 242-243 ◽  
Author(s):  
ANGELA S. TAVERNOR ◽  
W.R. ALLEN ◽  
G. W. BUTCHER
Keyword(s):  
Polymerase Chain Reaction ◽  
Cdna Cloning ◽  
Interleukin 2 ◽  
Chain Reaction ◽  
Polymerase Chain

cDNA cloning of porcine interleukin 2 by polymerase chain reaction

1991 ◽  
Vol 1089 (2) ◽  
pp. 257-258 ◽  
Author(s):  
Jane C. Goodall ◽  
David C. Emery ◽  
Michael Bailey ◽  
Leonard S. English ◽  
Len Hall
Keyword(s):  
Polymerase Chain Reaction ◽  
Cdna Cloning ◽  
Interleukin 2 ◽  
Chain Reaction ◽  
Polymerase Chain

Polymerase Chain Reaction Based cDNA Cloning of Wheat Profilin: A Potential Plant Allergen

1994 ◽  
Vol 105 (2) ◽  
pp. 190-194 ◽  
Author(s):  
H.-P. Rihs ◽  
P. Rozynek ◽  
K. May-Taube ◽  
B. Welticke ◽  
X. Baur
Keyword(s):  
Polymerase Chain Reaction ◽  
Cdna Cloning ◽  
Chain Reaction ◽  
Polymerase Chain

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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