An Alternative Quantitative Polymerase Chain Reaction Method

1996 ◽  
Vol 242 (1) ◽  
pp. 164
Author(s):  
Antonino Nicoletti ◽  
Caroline Sassy-Prigent
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
Polymerase Chain

scholarly journals Identification of Cervidae DNAin Feedstuff Using a Real-Time Polymerase Chain Reaction Method with the New Fluorescence Intercalating Dye EvaGreen

2009 ◽  
Vol 92 (1) ◽  
pp. 175-180 ◽  
Author(s):  
Ying Chen ◽  
Yajun Wu ◽  
Jing Wang ◽  
Baoliang Xu ◽  
Zhengyu Zhong ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
Meat Meal ◽  
Hair Samples ◽  
Intercalating Dye ◽  
Polymerase Chain

Abstract A real-time qualitative and quantitative polymerase chain reaction method (cer-194) using the fluorescence dye EvaGreen and aimed at the cytochrome b sequence was established for detection of cervidae DNA in feedstuff. Eight meat meal samples derived from deer, bovine, ovine, camel, pig, rabbit, fish, and chicken and 17 cervidae hair samples covering 2 subfamilies, 4 genera, and 7 species were tested to prove the specificity of the cer-194 system and its universality within the cervidae family. Detection limit of 0.1 deer meat in fish meal, blood powder, and feather powder matrixes was confirmed.

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