Polymerase Chain Reaction–Single-Strand Conformation Polymorphism Based Determination of Two Major Genetic Defects Responsible for a Phenotypic Polymorphism of Cytochrome P450 (CYP) 2C19 in the Japanese Population

2000 ◽  
Vol 284 (1) ◽  
pp. 160-162 ◽  
Author(s):  
Kunihiko Itoh ◽  
Kazuyuki Inoue ◽  
Hiroshi Nakao ◽  
Seiko Yanagiwara ◽  
Hitoshi Tada ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cytochrome P450 ◽  
Japanese Population ◽  
Single Strand Conformation Polymorphism ◽  
Genetic Defects ◽  
Chain Reaction ◽  
Phenotypic Polymorphism ◽  
Polymerase Chain ◽  
Conformation Polymorphism

A963G single nucleotide variant of BMP15 is not common bio-marker for fecundity in goat

2016 ◽  
Author(s):  
Guang-Xin E ◽  
Yong-Fu Huang ◽  
Jian-Ning He ◽  
Wei-Wei Ni ◽  
Yong-Ju Zhao
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Strand Conformation Polymorphism ◽  
Single Nucleotide Variant ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Exon 2 ◽  
Morphogenetic Protein ◽  
Polymerase Chain ◽  
Bone Morphogenetic Protein 15 ◽  
Conformation Polymorphism

Bone morphogenetic protein 15 (BMP15) is crucial factor for ovulation as well as for increasing litter size. In the present investigation efforts had been carried out to assess the genetic variations in Exon 2 region of BMP15 in goat, using polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) sequencing methods and cooperated frequency distribution to discuss its possibility of related fecundity. Across the 144samples from six breeds were identified in the A963G location of BMP15 using PCR-SSCP and sequences technology. A963A genotype was the most frequent (85.4%) and G963G was the least frequent with a frequency of 4.2% and A963G is 10.4%. It revealed non significant different between high and low fecundity breed. Therefore, this single nucleotide variant is not common Bio-Marker for fecundity in Goat.

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