Development of an Androgen Reporter Gene Assay (AR-LUX) Utilizing a Human Cell Line with an Endogenously Regulated Androgen Receptor

2001 ◽  
Vol 298 (1) ◽  
pp. 93-102 ◽  
Author(s):  
B.M.G. Blankvoort ◽  
E.M. de Groene ◽  
A.P. van Meeteren-Kreikamp ◽  
R.F. Witkamp ◽  
R.J.T. Rodenburg ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Cell Line ◽  
Reporter Gene ◽  
Human Cell ◽  
Human Cell Line ◽  
Reporter Gene Assay ◽  
Gene Assay

scholarly journals Selective Activation of Zebrafish Estrogen Receptor Subtypes by Chemicals by Using Stable Reporter Gene Assay Developed in a Zebrafish Liver Cell Line

2011 ◽  
Vol 125 (2) ◽  
pp. 439-449 ◽  
Author(s):  
Anne Cosnefroy ◽  
François Brion ◽  
Emmanuelle Maillot-Maréchal ◽  
Jean-Marc Porcher ◽  
Farzad Pakdel ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Cell Line ◽  
Reporter Gene ◽  
Liver Cell ◽  
Reporter Gene Assay ◽  
Receptor Subtypes ◽  
Selective Activation ◽  
Liver Cell Line ◽  
Gene Assay ◽  
Zebrafish Liver

Androgen receptor specificity and growth response of a human cell line (NHIK 3025)

1978 ◽  
Vol 11 (3) ◽  
pp. 309-323 ◽  
Author(s):  
E. Mulder ◽  
M.J. Peters ◽  
J. De Vries ◽  
H.J. Van Der Molen ◽  
K. Østgaard ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Cell Line ◽  
Human Cell ◽  
Growth Response ◽  
Human Cell Line ◽  
Receptor Specificity

Downscaling procedures reduce chemical use in androgen receptor reporter gene assay

2016 ◽  
Vol 571 ◽  
pp. 826-833 ◽  
Author(s):  
Carolina Di Paolo ◽  
Kristina Kirchner ◽  
Fabian Gerhard Peter Balk ◽  
Matthias Muschket ◽  
Werner Brack ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Gene Assay

scholarly journals Establishment of a Stable Human Cell Line, HPL-A3, for Use in Reporter Gene Assays of Cytochrome P450 3A Inducers

2012 ◽  
Vol 35 (5) ◽  
pp. 677-685 ◽  
Author(s):  
Masashi Sekimoto ◽  
Shinsuke Sano ◽  
Takuomi Hosaka ◽  
Kiyomitsu Nemoto ◽  
Masakuni Degawa
Keyword(s):  
Cytochrome P450 ◽  
Cell Line ◽  
Reporter Gene ◽  
Human Cell ◽  
Human Cell Line ◽  
Cytochrome P450 3A ◽  
Reporter Gene Assays

Steroid responsiveness of the human cell line NHIK 3025

1981 ◽  
Vol 97 (4) ◽  
pp. 551-558
Author(s):  
Kjetill Østgaard ◽  
Einar Wibe ◽  
Kristen B. Eik-Nes
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Androgen Receptor ◽  
Cell Growth ◽  
Cell Line ◽  
Human Cell ◽  
Calf Serum ◽  
Human Cell Line ◽  
Foetal Calf Serum ◽  
Steroid Responsiveness

Abstract. The human cell line NHIK 3025, derived from a carcinoma of the uterine cervix, contains a glucocorticoid and an androgen receptor. The effect of various natural and synthetic steroid hormones and antihormones on growth rate of these cells was therefore investigated. Cells grown in Eagle's MEM with 10% foetal calf serum exhibited reduced growth when cultured with dexamethasone due to prolongation of the cell cycle. Glucocorticoid anti-inducers like progesterone had no significant effect on cell growth. Methyltrienolone (R 1881) or 5α-dihydrotestosterone did not affect cell proliferation. The reported shortening of the cell cycle by testosterone is probably not directly connected with activation of the androgen receptor present, but possibly dependent on metabolic conversion of testosterone to the more potent growth stimulator 4-androstene-3β, 17β-diol. The effect of several anti-androgens was also studied. The non-steroidal anti-androgens flutamide and SCH 16483 had no significant effect on cell proliferation. It was, however, found that a number of steroid antiandrogens, including R2956, stimulated cell growth. A significant stimulatory effect by R2956 was seen within the first cell generation, 4-androstene-3β,17β-diol had to be present during 2 days, and testosterone for even longer times before a similar effect on cell growth could be obtained.

Androgen receptor activities of p,p′-DDE, fenvalerate and phoxim detected by androgen receptor reporter gene assay

2006 ◽  
Vol 160 (2) ◽  
pp. 151-157 ◽  
Author(s):  
Li-Chun Xu ◽  
Hong Sun ◽  
Jian-Feng Chen ◽  
Qian Bian ◽  
Ling Song ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Gene Assay

Identification and characterization of human interferon alpha inhibitors through a WISH cell line-based reporter gene assay

2020 ◽  
Vol 94 ◽  
pp. 103372
Author(s):  
Milagros Bürgi ◽  
Paola Hernández ◽  
Mauricio Cabrera ◽  
Hugo Cerecetto ◽  
Mercedes González ◽  
...  
Keyword(s):  
Cell Line ◽  
Reporter Gene ◽  
Interferon Alpha ◽  
Reporter Gene Assay ◽  
Human Interferon ◽  
Gene Assay ◽  
Identification And Characterization
Sign in / Sign up

Export Citation Format

Share Document