Phospholipid Composition of Cell-Derived Microparticles Determined by One-Dimensional High-Performance Thin-Layer Chromatography

2002 ◽  
Vol 302 (2) ◽  
pp. 191-198 ◽  
Author(s):  
A.M. Weerheim ◽  
A.M. Kolb ◽  
A. Sturk ◽  
R. Nieuwland
1980 ◽  
Vol 26 (3) ◽  
pp. 403-405
Author(s):  
M D Kolins ◽  
E Epstein ◽  
W H Civin ◽  
S Weiner

Abstract We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations.


1980 ◽  
Vol 26 (3) ◽  
pp. 403-405 ◽  
Author(s):  
M D Kolins ◽  
E Epstein ◽  
W H Civin ◽  
S Weiner

Abstract We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations.


1993 ◽  
Vol 645 (1) ◽  
pp. 189-192 ◽  
Author(s):  
Sherry A. Leonard ◽  
So Chun Wong ◽  
Jonathan W. Nyce

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