Hydrocortisone Reinforces the Blood–Brain Barrier Properties in a Serum Free Cell Culture System

1998 ◽  
Vol 244 (1) ◽  
pp. 312-316 ◽  
Author(s):  
Dirk Hoheisel ◽  
Thorsten Nitz ◽  
Helmut Franke ◽  
Joachim Wegener ◽  
Ansgar Hakvoort ◽  
...  
Keyword(s):  
Cell Culture ◽  
Blood Brain Barrier ◽  
Culture System ◽  
Barrier Properties ◽  
Free Cell ◽  
Brain Barrier ◽  
Cell Culture System ◽  
Serum Free ◽  
Blood Brain

Serum-free cell culture system supplemented with lipid-rich albumin for hepatitis C virus (strain O of genotype 1b) replication

2007 ◽  
Vol 125 (2) ◽  
pp. 162-168 ◽  
Author(s):  
Ken-ichi Abe ◽  
Masanori Ikeda ◽  
Yasuo Ariumi ◽  
Hiromichi Dansako ◽  
Nobuyuki Kato
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Strain ◽  
Culture System ◽  
Free Cell ◽  
Cell Culture System ◽  
Serum Free ◽  
Genotype 1B

Generation of murine bone marrow derived macrophages in a standardised serum-free cell culture system

2009 ◽  
Vol 342 (1-2) ◽  
pp. 13-19 ◽  
Author(s):  
Kristin Eske ◽  
Katrin Breitbach ◽  
Jens Köhler ◽  
Patimaporn Wongprompitak ◽  
Ivo Steinmetz
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Culture System ◽  
Free Cell ◽  
Cell Culture System ◽  
Serum Free ◽  
Murine Bone Marrow

scholarly journals In vitro models of the blood–brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use

2016 ◽  
Vol 36 (5) ◽  
pp. 862-890 ◽  
Author(s):  
Hans C Helms ◽  
N Joan Abbott ◽  
Malgorzata Burek ◽  
Romeo Cecchelli ◽  
Pierre-Olivier Couraud ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cell ◽  
Blood Brain Barrier ◽  
Brain Parenchyma ◽  
Brain Barrier ◽  
Blood Brain ◽  
Culture Models ◽  
Cell Culture Models ◽  
The Brain

The endothelial cells lining the brain capillaries separate the blood from the brain parenchyma. The endothelial monolayer of the brain capillaries serves both as a crucial interface for exchange of nutrients, gases, and metabolites between blood and brain, and as a barrier for neurotoxic components of plasma and xenobiotics. This “blood-brain barrier” function is a major hindrance for drug uptake into the brain parenchyma. Cell culture models, based on either primary cells or immortalized brain endothelial cell lines, have been developed, in order to facilitate in vitro studies of drug transport to the brain and studies of endothelial cell biology and pathophysiology. In this review, we aim to give an overview of established in vitro blood–brain barrier models with a focus on their validation regarding a set of well-established blood–brain barrier characteristics. As an ideal cell culture model of the blood–brain barrier is yet to be developed, we also aim to give an overview of the advantages and drawbacks of the different models described.

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