Analysis of Cytokine Production in the Colon of Nude Mice with Experimental Colitis Induced by Adoptive Transfer of Immunocompetent Cells from Mice Infected with a Murine Retrovirus

Studies showed that specific probiotics provide therapeutic benefits in inflammatory bowel disease.In vitroevidence suggested thatLactobacillus paracaseialso called ST11 (CNCM I-2116) is a potent strain with immune modulation properties. However, little is known about its capacity to alleviate inflammatory symptomsin vivoIn this context, the main objective of this study was to investigate the role of ST11 on intestinal inflammation using the adoptive transfer mouse model of experimental colitis. Rag2-/-recipient mice were fed with ST11 (109CFU/day)a month prior toinduce colitis by adoptive transfer of naive T cells. One month later, in clear contrast to nonfed mice, weight loss was significantly reduced by 50% in ST11-fed mice. Further analysis of colon specimens revealed a significant reduction neutrophil infiltration and mucosal expression of IL1β, IL-6, and IL12 proinflammatory cytokines, whereas no consistent differences in expression of antibacterial peptides or tight junction proteins were observed between PBS and ST11-fed mice. All together, our results demonstrate that oral administration of ST11 was safe and had a significant preventive effect on colitis. We conclude that probiotics such asLactobacillus paracaseiharbor worthwhilein vivoimmunomodulatory properties to prevent intestinal inflammation by nutritional approaches.

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Endotoxaemia and cytokine production in experimental colitis

British Journal of Surgery ◽

10.1002/bjs.1800821110 ◽

1995 ◽

Vol 82 (11) ◽

pp. 1479-1482 ◽

Cited By ~ 27

Author(s):

P. J. D. Neilly ◽

K. R. Gardiner ◽

S. J. Kirk ◽

G. Jennings ◽

N. H. Anderson ◽

...

Keyword(s):

Cytokine Production ◽

Experimental Colitis

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Recovery of Nude Mice from Sendai Virus Infection after Adoptive Transfer of Spleen T Cells or T Cell-Depleted Spleen Cells

Microbiology and Immunology ◽

10.1111/j.1348-0421.1983.tb00605.x ◽

1983 ◽

Vol 27 (5) ◽

pp. 465-469 ◽

Cited By ~ 5

Author(s):

Hiroshi Iwai ◽

Katsumoto Ueda ◽

Muneo Saito

Keyword(s):

T Cells ◽

T Cell ◽

Virus Infection ◽

Nude Mice ◽

Adoptive Transfer ◽

Sendai Virus ◽

Spleen Cells

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Activation of the Epidermal Growth Factor Receptor in Macrophages Regulates Cytokine Production and Experimental Colitis

The Journal of Immunology ◽

10.4049/jimmunol.1300133 ◽

2014 ◽

Vol 192 (3) ◽

pp. 1013-1023 ◽

Cited By ~ 46

Author(s):

Ning Lu ◽

Lihong Wang ◽

Hailong Cao ◽

Liping Liu ◽

Luc Van Kaer ◽

...

Keyword(s):

Epidermal Growth Factor Receptor ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Cytokine Production ◽

Growth Factor Receptor ◽

Experimental Colitis ◽

Epidermal Growth

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Cancer Prevention by Adoptive Transfer of Antigen 60‐Activated Immunocompetent Cells

Scandinavian Journal of Immunology ◽

10.1046/j.1365-3083.1996.d01-37.x ◽

1996 ◽

Vol 43 (3) ◽

pp. 283-288 ◽

Cited By ~ 2

Author(s):

H. MAES ◽

C COCITO

Keyword(s):

Cancer Prevention ◽

Adoptive Transfer ◽

Immunocompetent Cells ◽

Antigen 60

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Characterization of immunologic tolerance induced by transfusion of UV-B–irradiated allogeneic mononuclear leukocytes

Blood ◽

10.1182/blood.v98.4.1239 ◽

2001 ◽

Vol 98 (4) ◽

pp. 1239-1245 ◽

Cited By ~ 14

Author(s):

Kuo-Jang Kao ◽

Eileen S. Huang ◽

Sandra Donahue

Keyword(s):

T Cells ◽

Immune Tolerance ◽

Adoptive Transfer ◽

Cd4 T Cells ◽

Cytokine Production ◽

Tolerance Induction ◽

Mononuclear Leukocytes ◽

Dependent Manner ◽

Regulatory Activity ◽

Cba Mice

Transfusions of UV-B–irradiated peripheral blood mononuclear cells (UV-B–PBMCs) from BALB/c (H-2d) mice into CBA (H-2k) mice can induce humoral immune tolerance to H-2d antigens, and the induced tolerance is partially mediated by negative regulatory PBMCs. To further identify which subset of spleen mononuclear leukocytes (MNLs) in the tolerant CBA mice is responsible for the negative regulatory activity, adoptive transfer experiments were conducted using spleen MNLs from the tolerant CBA mice. Results showed that only CD4+ T cells could transfer the negative regulatory activity in a dose-dependent manner. This negative regulatory activity was significantly reduced when CD25+ helper T cells were removed. Further study suggested that inhibition of IL-12 production by UV-B–irradiated PBMCs played a role in the induction of immune tolerance. In vitro study of the cytokine production profile by CBA CD4+ T cells, after stimulation with gamma-irradiated BALB/c spleen cells, revealed an enhanced production of the type 2 T-cell cytokines after tolerance induction. Induction of tolerance also prevented the development of cytotoxic T cells in CBA mice against BALB/c MNLs. Adoptive transfer study suggested that the cellular immune tolerance was also mediated by CD4+ negative regulatory T cells. The induced immune tolerance was nullified after 400 cGy sublethal gamma irradiation. These results suggest that the ex vivo study of cytokine production by T cells may be used to monitor tolerance induction and the selection of gamma radiation dose is critical for potential clinical application of the tolerance induced by UV-B–PBMCs.

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