Expression of a Novel Neuropeptide Y Receptor Subtype Involved in Food Intake: An in Situ Hybridization Study of Y5 mRNA Distribution in Rat Brain

2000 ◽  
Vol 165 (1) ◽  
pp. 90-100 ◽  
Author(s):  
Margaret M. Durkin ◽  
Mary W. Walker ◽  
Kelli E. Smith ◽  
Eric L. Gustafson ◽  
Christophe Gerald ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Food Intake ◽  
Neuropeptide Y ◽  
Rat Brain ◽  
Receptor Subtype ◽  
Hybridization Study ◽  
Neuropeptide Y Receptor

An in situ hybridization study on interleukin-1β mRNA induced by transient forebrain ischemia in the rat brain

1994 ◽  
Vol 26 (1-2) ◽  
pp. 135-142 ◽  
Author(s):  
Kazuki Yabuuchi ◽  
Masabumi Minami ◽  
Seishi Katsumata ◽  
Akira Yamazaki ◽  
Masamichi Satoh
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Interleukin 1Β ◽  
Forebrain Ischemia ◽  
Hybridization Study ◽  
Transient Forebrain Ischemia

scholarly journals In situ hybridization study of IL-1β mRNA induced by kainic acid in rat brain

1992 ◽  
Vol 58 ◽  
pp. 60
Author(s):  
Kazuki Yabuuchi ◽  
Masabumi Minami ◽  
Yasushi Kuraishi ◽  
Masamichi Satoh
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Kainic Acid ◽  
Hybridization Study

scholarly journals LGR4 and Its Ligands, R-Spondin 1 and R-Spondin 3, Regulate Food Intake in the Hypothalamus of Male Rats

Endocrinology ◽  
2014 ◽  
Vol 155 (2) ◽  
pp. 429-440 ◽  
Author(s):  
Ji-Yao Li ◽  
Biaoxin Chai ◽  
Weizhen Zhang ◽  
Danielle M. Fritze ◽  
Chao Zhang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Food Intake ◽  
Neuropeptide Y ◽  
Paraventricular Nucleus ◽  
Median Eminence ◽  
Energy Homeostasis ◽  
Male Rats ◽  
The Third ◽  
The Brain

The hypothalamus plays a key role in the regulation of feeding behavior. Several hypothalamic nuclei, including the arcuate nucleus (ARC), paraventricular nucleus, and ventromedial nucleus of the hypothalamus (VMH), are involved in energy homeostasis. Analysis of microarray data derived from ARC revealed that leucine-rich repeat-containing G protein-coupled receptor 4 (LGR4) is highly expressed. LGR4, LGR5, and LGR6 form a subfamily of closely related receptors. Recently, R-spondin (Rspo) family proteins were identified as ligands of the LGR4 subfamily. In the present study, we investigated the distribution and function of LGR4–LGR6 and Rspos (1–4) in the brain of male rat. In situ hybridization showed that LGR4 is expressed in the ARC, VMH, and median eminence of the hypothalamus. LGR4 colocalizes with neuropeptide Y, proopiomelanocortin, and brain-derived neurotrophic factor neurons. LGR5 is not detectable with in situ hybridization; LGR6 is only expressed in the epithelial lining of the lower portion of the third ventricle and median eminence. Rspo1 is expressed in the VMH and down-regulated with fasting. Rspo3 is expressed in the paraventricular nucleus and also down-regulated with fasting. Rspos 1 and 3 colocalize with the neuronal marker HuD, indicating that they are expressed by neurons. Injection of Rspo1 or Rspo3 into the third brain ventricle inhibited food intake. Rspo1 decreased neuropeptide Y and increased proopiomelanocortin expression in the ARC. Rspo1 and Rspo3 mRNA is up-regulated by insulin. These data indicate that Rspo1 and Rspo3 and their receptor LGR4 form novel circuits in the brain to regulate energy homeostasis.

Distribution of thyrotropin-releasing hormone receptor messenger RNA in the rat brain: An in situ hybridization study

Neuroscience ◽  
1992 ◽  
Vol 51 (4) ◽  
pp. 891-909 ◽  
Author(s):  
L. Calzá ◽  
L. Giardino ◽  
S. Ceccatelli ◽  
M. Zanni ◽  
R. Elde ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Hormone Receptor ◽  
Messenger Rna ◽  
Thyrotropin Releasing Hormone ◽  
Hybridization Study ◽  
Releasing Hormone
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