Isolation and Characterization of the Goldfish Thyrotropin β Subunit Gene Including the 5′-Flanking Region

1999 ◽  
Vol 115 (3) ◽  
pp. 463-473 ◽  
Author(s):  
Young Chang Sohn ◽  
Yasutoshi Yoshiura ◽  
Hiroaki Suetake ◽  
Makito Kobayashi ◽  
Katsumi Aida
Keyword(s):  
Β Subunit ◽  
Subunit Gene ◽  
Isolation And Characterization ◽  
Flanking Region

Isolation and Characterization of the Yeast mRNA Capping Enzyme β Subunit Gene Encoding RNA 5′-Triphosphatase, Which Is Essential for Cell Viability

1997 ◽  
Vol 239 (1) ◽  
pp. 116-122 ◽  
Author(s):  
Toshihiko Tsukamoto ◽  
Yoshio Shibagaki ◽  
Shinobu Imajoh-Ohmi ◽  
Teruko Murakoshi ◽  
Masako Suzuki ◽  
...  
Keyword(s):  
Cell Viability ◽  
Β Subunit ◽  
Subunit Gene ◽  
Gene Encoding ◽  
Isolation And Characterization ◽  
Mrna Capping ◽  
Capping Enzyme

Genomic organization of the 5′ end of human β-ENaC and preliminary characterization of its promoter

2002 ◽  
Vol 282 (5) ◽  
pp. F898-F909 ◽  
Author(s):  
Christie P. Thomas ◽  
Randy W. Loftus ◽  
Kang Z. Liu ◽  
Omar A. Itani
Keyword(s):  
Genomic Organization ◽  
Human Kidney ◽  
Β Subunit ◽  
Transcription Start ◽  
Transcription Start Sites ◽  
Nuclear Extracts ◽  
Flanking Region ◽  
Rapid Amplification ◽  
Flanking Regions

The mRNA for the β-subunit of the epithelial Na+ channel (β-ENaC) is regulated developmentally and, in some tissues, in response to corticosteroids. To understand the mechanisms of transcriptional regulation of the human β-ENaC gene, we characterized the 5′ end of the gene and its 5′-flanking regions. Adaptor-ligated human kidney and lung cDNA were amplified by 5′ rapid amplification of cDNA ends, and transcription start sites of two 5′ variant transcripts were determined by nuclease protection or primer extension assays. Cosmid clones that contain the 5′ end of the gene were isolated, and analysis of these clones indicated that alternate first exons ∼1.5 kb apart and ∼ 45 kb upstream of a common second exon formed the basis of these transcripts. Genomic fragments that included the proximal 5′-flanking region of either transcript were able to direct expression of a reporter gene in lung epithelia and to bind Sp1 in nuclear extracts, confirming the presence of separate promoters that regulate β-ENaC expression.

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