Selection of an Isolate of Zoophthora radicans (Zygomycetes: Entomophthorales) for Biocontrol of the Diamondback Moth Plutella xylostella (Lepidoptera: Yponomeutidae)

1993 ◽  
Vol 61 (1) ◽  
pp. 75-80 ◽  
Author(s):  
J.K. Pell ◽  
N. Wilding ◽  
A.L. Player ◽  
S.J. Clark
2016 ◽  
Vol 132 ◽  
pp. 38-46 ◽  
Author(s):  
Shuzhen Zhang ◽  
Xiaolei Zhang ◽  
Jun Shen ◽  
Kaikai Mao ◽  
Hong You ◽  
...  

1997 ◽  
Vol 87 (5) ◽  
pp. 481-486 ◽  
Author(s):  
M. Iqbal ◽  
D.J. Wright

AbstractThe efficacy of abamectin (AgrimecR) and teflubenzuron (NomoltR) was assessed by leaf-dip bioassay against larvae of the diamondback moth, Plutella xylostella Linnaeus from a population (SERD3) collected originally in lowland Malaysia in December 1994. Evidence for resistance to both abamectin and teflubenzuron was found in the F7 generation (LC50 ratio of 60 and 24 respectively compared with a laboratory, insecticide-susceptible strain). Selection of sub-populations of SERD3 (F7–F9) with abamectin and teflubenzuron increased the LC50 ratio to 220 and 360 respectively and estimates of realized heritability [h2] were high (c. 0.8 and 0.9) for both compounds. There was no cross-resistance between these compounds in the abamectin and teflubenzuron-selected sub-populations but some indication of negatively-correlated resistance. Topical application of the synergists piperonyl butoxide, S,S,S-tributylphosphorotrithioate and maleic acid diethyl ester to the laboratory strain had no significant effect on the toxicity of abamectin or teflubenzuron in subsequent leaf-dip assays. In contrast, pre-treatment with piperonyl butoxide and S,S,S-tributylphosphorotrithioate significantly increased the toxicity of abamectin (c. 4- and 3-fold) and teflubenzuron (c. 7- and 19-fold) in the abamectin and teflubenzuron-selected sub-populations of SERD3, suggesting that microsomal monoxygenases and/or esterases may be involved in resistance. Pre-treatment with maleic acid diethyl ester only increased the toxicity of abamectin by c. 2-fold and had no significant effect on the toxicity of teflubenzuron, providing limited evidence for the involvement of glutathione-S-transferases in resistance to the former compound alone.


2008 ◽  
Vol 26 (2) ◽  
pp. 259-261 ◽  
Author(s):  
Dirceu Pratissoli ◽  
Ricardo A Polanczyk ◽  
Anderson M Holtz ◽  
Leandro P Dalvi ◽  
Alexandre F Silva ◽  
...  

Biological characteristics of the egg parasitoids Trichogramma acacioi, T. atopovirilia, and T. bennetti were studied to select the one with better performance on the control of the Diamondback moth, Plutella xylostella. Parasitism rate, viability, and sex ratio were studied. Parasitism rate ranged from 1.67 to 41.33%. T. atopovirilia and T. acacioi were respectively the most and less aggressive species. The viability ranged from 6.20% for T. acacioi to 53.34% for T. atopovirilia. There were no differences on sex ratio (p<0.05) that remained above 0,88. T. atopovirilia was the species with best performance in the laboratory when rearing on the Diamondback moth eggs.


Author(s):  
Amanda A F Lemes ◽  
Thamiris P Sipriano-Nascimento ◽  
Natalia F Vieira ◽  
Camila P Cardoso ◽  
Alessandra M Vacari ◽  
...  

Abstract The diamondback moth, Plutella xylostella (Linnaeus, 1758), is one of the main pests of brassicas, and various insecticides, such as indoxacarb, are used to control it. However, frequent insecticide applications favor the selection of resistant individuals. Thus, the residual and sublethal effects of indoxacarb in two populations of P. xylostella, one collected in the field (FP) and one from the laboratory (LP), were evaluated and compared. The objective of this research was to investigate the toxicity of indoxacarb at residual and sublethal levels in a field population of P. xylostella from Brazil and a population from the laboratory. Leaf-dip bioassays showed high toxicity, with LC50 values after 48 h of 3.7 and 6.9 mg/liter for the LP and FP, respectively. Sublethal effects were indicated by significant reduction in the survival of larvae, pupae, and offspring. There was an increase in foliar consumption and a decrease in adult survival in the LP, and a decrease in fecundity in the FP. For the LP and FP population, the mean values for R0, rm, and λ for the control treatment were significantly higher than for the treatment groups (CL15 and CL25). Exposure of larvae to sublethal indoxacarb concentrations significantly reduced larval and pupal survival. Larval and pupal survival decreased as the indoxacarb concentration increased. Fecundity was significantly lower for the FP at LC15 (96.2 eggs per female) and LC25 (69.2 eggs per female) concentrations compared with the other treatments.


2001 ◽  
Vol 54 ◽  
pp. 47-50 ◽  
Author(s):  
H. Yeo ◽  
J.K. Pell ◽  
M. Walter ◽  
K.S.H. Boyd-Wilson ◽  
C. Snelling ◽  
...  

The fungus Zoophthora radicans is being developed as a biopesticide for control of diamondback moth (Plutella xylostella) A series of laboratory doseresponse bioassays was used to determine the virulence of five isolates of Z radicans to diamondback moths indigenous to New Zealand A reference isolate from Malaysia was compared to four isolates from New Zealand Late second and early third instar diamondback moth larvae were exposed to discs of broccoli leaves that had previously been inoculated with increasing densities of fungal conidia Isolates from New Zealand were consistently more infective than the Malaysian isolate LC50 values ranged from 092 conidia/mm2 for isolate NW337 (New Zealand) to 697 conidia/mm2 for NW250 (Malaysia) Resting spores were found in up to 30 of the total number of larvae that succumbed to infection with the New Zealand isolates but were never found in larvae that succumbed to infection with isolate NW250 A single isolate was identified with potential for future testing under field conditions


2008 ◽  
Vol 26 (2) ◽  
pp. 194-196
Author(s):  
Dirceu Pratissoli ◽  
Ricardo A Polanczyk ◽  
Anderson M Holtz ◽  
Leandro P Dalvi ◽  
Alexandre F Silva ◽  
...  

Biological characteristics of the egg parasitoids Trichogramma acacioi, T. atopovirilia, and T. bennetti were studied to select the one with better performance on the control of the Diamondback moth, Plutella xylostella. Parasitism rate, viability, and sex ratio were studied. Parasitism rate ranged from 1.67 to 41.33%. T. atopovirilia and T. acacioi were respectively the most and less aggressive species. The viability ranged from 6.20% for T. acacioi to 53.34% for T. atopovirilia. There were no differences on sex ratio (P < 0.05) that remained above 0,88. T. atopovirilia was the species with best performance in the laboratory when rearing on the Diamondback moth eggs.


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