Identification of Protein-Protein interactions of the major sperm protein (MSP) of Caenorhabditis elegans

1998 ◽  
Vol 279 (3) ◽  
pp. 605-619 ◽  
Author(s):  
Harold E Smith ◽  
Samuel Ward
Keyword(s):  
Caenorhabditis Elegans ◽  
Protein Interactions ◽  
Major Sperm Protein ◽  
Protein Protein Interactions ◽  
Sperm Protein

scholarly journals Genetic and Molecular Characterization of the Caenorhabditis elegans Gene, mel-26, a Postmeiotic Negative Regulator of MEI-1, a Meiotic-Specific Spindle Component

Genetics ◽  
1998 ◽  
Vol 150 (1) ◽  
pp. 119-128
Author(s):  
M Rhys Dow ◽  
Paul E Mains
Keyword(s):  
Caenorhabditis Elegans ◽  
Protein Interactions ◽  
Negative Regulator ◽  
Meiotic Spindle ◽  
Loss Of Function ◽  
Protein Protein Interactions ◽  
Gain Of Function ◽  
Recessive Mutations ◽  
Female Germline

Abstract We have previously described the gene mei-1, which encodes an essential component of the Caenorhabditis elegans meiotic spindle. When ectopically expressed after the completion of meiosis, mei-1 protein disrupts the function of the mitotic cleavage spindles. In this article, we describe the cloning and the further genetic characterization of mel-26, a postmeiotic negative regulator of mei-1. mel-26 was originally identified by a gain-of-function mutation. We have reverted this mutation to a loss-of-function allele, which has recessive phenotypes identical to the dominant defects of its gain-of-function parent. Both the dominant and recessive mutations of mel-26 result in mei-1 protein ectopically localized in mitotic spindles and centrosomes, leading to small and misoriented cleavage spindles. The loss-of-function mutation was used to clone mel-26 by transformation rescue. As suggested by genetic results indicating that mel-26 is required only maternally, mel-26 mRNA was expressed predominantly in the female germline. The gene encodes a protein that includes the BTB motif, which is thought to play a role in protein-protein interactions.

scholarly journals Major sperm protein signaling promotes oocyte microtubule reorganization prior to fertilization in Caenorhabditis elegans

2006 ◽  
Vol 299 (1) ◽  
pp. 105-121 ◽  
Author(s):  
Jana E. Harris ◽  
J. Amaranath Govindan ◽  
Ikuko Yamamoto ◽  
Joel Schwartz ◽  
Irina Kaverina ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Protein Signaling ◽  
Major Sperm Protein ◽  
Sperm Protein

Intragenic dominant suppressors of glp-1, a gene essential for cell-signaling in Caenorhabditis elegans, support a role for cdc10/SWI6/ankyrin motifs in GLP-1 function.

Genetics ◽  
1993 ◽  
Vol 135 (4) ◽  
pp. 1023-1034 ◽  
Author(s):  
J L Lissemore ◽  
P D Currie ◽  
C M Turk ◽  
E M Maine
Keyword(s):  
Caenorhabditis Elegans ◽  
Cell Fate ◽  
Protein Interactions ◽  
Protein S ◽  
Single Strand Conformation Polymorphism ◽  
Specific Protein ◽  
Temperature Sensitive ◽  
Protein Protein Interactions ◽  
Ts Mutations ◽  
Dominant Suppressor

Abstract The glp-1 gene product mediates cell-cell interactions required for cell fate specification during development in Caenorhabditis elegans. To identify genes that interact with glp-1, we screened for dominant suppressors of two temperature-sensitive glp-1 alleles and recovered 18 mutations that suppress both germline and embryonic glp-1 phenotypes. These dominant suppressors are tightly linked to glp-1 and do not bypass the requirement for a distal tip cell, which is thought to be the source of a signal that is received and transduced by the GLP-1 protein. Using single-strand conformation polymorphism (SSCP) analysis and DNA sequencing, we found that at least 17 suppressors are second-site intragenic revertants. The suppressors, like the original glp-1(ts) mutations, are all located in the cdc10/SWI6/ankyrin domain of GLP-1. cdc10/SWI6/ankyrin motifs have been shown to mediate specific protein-protein interactions in other polypeptides. We propose that the glp-1(ts) mutations disrupt contact between GLP-1 and an as yet unidentified target protein(s) and that the dominant suppressor mutations restore appropriate protein-protein interactions.

scholarly journals The Caenorhabditis elegans spe-6 gene is required for major sperm protein assembly and shows second site non-complementation with an unlinked deficiency.

Genetics ◽  
1993 ◽  
Vol 133 (1) ◽  
pp. 79-86 ◽  
Author(s):  
J P Varkey ◽  
P L Jansma ◽  
A N Minniti ◽  
S Ward
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Product ◽  
Spindle Pole ◽  
Specific Gene ◽  
Major Sperm Protein ◽  
Sperm Protein ◽  
Fibrous Body ◽  
Sperm Development ◽  
Chromosome Iii

Abstract Caenorhabditis elegans spermatozoa move by crawling. Their motility requires thin cytoskeletal filaments assembled from a unique cytoskeletal protein, the major sperm protein (MSP). During normal sperm development the MSP is segregated to developing sperm by assembly into filaments that form a paracrystalline array in a transient organelle, the fibrous body-membranous organelle. Mutations in the spe-6 gene cause sterility because they lead to defective primary spermatocytes that do not form spermatids. In these mutant spermatocytes the MSP fails to assemble into fibrous body filaments. Instead, the unassembled MSP distributes throughout the cytoplasm and nucleus. Thus, the spe-6 gene product is necessary for normal MSP localization and assembly during sperm development. In addition to their MSP assembly defect, spe-6 mutant spermatocytes arrest meiosis at diakinesis although their spindle pole bodies still replicate and separate. This results in spermatocytes with four half-spindles surrounding condensed, but unsegregated, chromosomes. All four spe-6 alleles, as well as a chromosome III deficiency that deletes the spe-6 gene, fail to complement two small overlapping chromosome IV deficiencies, eDf18 and eDf19. This non-allele-specific second site non-complementation suggests a concentration-dependent interaction between the spe-6 gene product and products of the gene(s) under eDf18 and eDf19, which include a cluster of sperm-specific genes. Since MSP filament assembly is highly concentration-dependent in vitro, the non-complementation might be expected if the sperm-specific gene products under eDf18 and eDf19 were needed together with the spe-6 gene product to promote MSP assembly.

scholarly journals Gene Cascade Finder: A tool for identification of gene cascades and its application in Caenorhabditis elegans

2019 ◽  
Author(s):  
Yusuke Nomoto ◽  
Yukihiro Kubota ◽  
Kota Kasahara ◽  
Aimi Tomita ◽  
Takehiro Oshime ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Cell Fate ◽  
Protein Interactions ◽  
Regulatory Networks ◽  
Cell Lineage ◽  
Rna Seq ◽  
Protein Protein Interactions ◽  
Gene Regulatory ◽  
Or Gene ◽  
Canonical Wnt

AbstractObtaining a comprehensive understanding of the gene regulatory networks, or gene cascades, involved in cell fate determination and cell lineage segregation in Caenorhabditis elegans is a long-standing challenge. Although RNA-sequencing (RNA-Seq) is a promising technique to resolve these questions, the bioinformatics tools to identify associated gene cascades from RNA-Seq data remain inadequate. To overcome these limitations, we developed Gene Cascade Finder (GCF) as a novel tool for building gene cascades by comparison of mutant and wild-type RNA-Seq data along with integrated information of protein-protein interactions, expression timing, and domains. Application of GCF to RNA-Seq data confirmed that SPN-4 and MEX-3 regulate the canonical Wnt pathway during embryonic development. Moreover, lin-35, hsp-3, and gpa-12 were found to be involved in MEX-1-dependent neurogenesis, and MEX-3 was found to control the gene cascade promoting neurogenesis through lin-35 and apl-1. Thus, GCF could be a useful tool for building gene cascades from RNA-Seq data.

Genomic organization of major sperm protein genes and pseudogenes in the nematode Caenorhabditis elegans

1988 ◽  
Vol 199 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Samuel Ward ◽  
Daniel J. Burke ◽  
John E. Sulston ◽  
Alan R. Coulson ◽  
Donna G. Albertson ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Genomic Organization ◽  
Major Sperm Protein ◽  
Nematode Caenorhabditis Elegans ◽  
Sperm Protein
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