Altering molecular recognition of RNA aptamers by allosteric selection

2000 ◽  
Vol 298 (4) ◽  
pp. 623-632 ◽  
Author(s):  
Garrett A Soukup ◽  
Gail A.M Emilsson ◽  
Ronald R Breaker
Keyword(s):  
Molecular Recognition ◽  
Rna Aptamers

scholarly journals The Dual Life of RNA

2004 ◽  
Vol 213 ◽  
pp. 315-320
Author(s):  
M. Meli ◽  
M-C. Maurel
Keyword(s):  
Molecular Recognition ◽  
Molecular Biology ◽  
Fluorescent Probes ◽  
Optical Signal ◽  
Rna Aptamers ◽  
Small Targets ◽  
Terrestrial Material

Molecular biology techniques have enabled us to prepare and select RNA aptamers that can bind specifically to small targets. RNA oligonucleotides can also be used as fluorescent probes. We have combined the two approaches to obtain Aptamer Beacons, in which molecular recognition is linked to the emission of an optical signal. These RNA biosensors could be used to detect directly the signatures of life in samples of mineral and extra-terrestrial material.

scholarly journals Molecular recognition of amino acids by RNA aptamers: The evolution into an L-tyrosine binder of a dopamine-binding RNA motif

RNA ◽  
2000 ◽  
Vol 6 (4) ◽  
pp. 520-527 ◽  
Author(s):  
CECILIA MANNIRONI ◽  
CHIARA SCERCH ◽  
PAOLO FRUSCOLONI ◽  
GLAUCO P. TOCCHINI-VALENTINI
Keyword(s):  
Amino Acids ◽  
Molecular Recognition ◽  
Rna Aptamers ◽  
Rna Motif

scholarly journals Molecular recognition elements - DNA/RNA-aptamers to proteins

2010 ◽  
Vol 56 (6) ◽  
pp. 639-656 ◽  
Author(s):  
V.A. Spiridonova
Keyword(s):  
Molecular Recognition ◽  
Specific Binding ◽  
Rna Aptamers ◽  
Nucleic Acid Binding ◽  
Wide Spread ◽  
Protein Target ◽  
Nucleic Acid Binding Proteins ◽  
Therapeutic Molecules ◽  
Recognition Elements ◽  
Protein Nucleic Acid

In this review summarizes data on DNA/RNA aptamers - a novel class of molecular recognition elements. Special attention is paid to the aptamers to proteins involved into pathogenesis of wide spread human diseases. These include aptamers to serine protease, to cytokines/growth factors, to influenza viral protein, nucleic acid binding proteins. Strong and specific binding for a given protein target of aptamers make them an attractive class of direct protein inhibitors. They can inhibit pathogenic proteins and it is becoming clear that aptamers have the potential to be a new and effective class of therapeutic molecules.

A Three-Dimensional Tetraphenylethylene-Based Fluorescence Covalent Organic Framework for Molecular Recognition

2020 ◽  
Author(s):  
Junxia Ren ◽  
Yaozu Liu ◽  
Xin Zhu ◽  
Yangyang Pan ◽  
Yujie Wang ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Fluorescence Probe ◽  
Three Dimensional ◽  
Hazardous Substances ◽  
Covalent Organic Framework ◽  
Highly Sensitive ◽  
Volatile Organic ◽  
Polycyclic Aromatic ◽  
Better Than ◽  
Organic Framework

<p><a></a><a></a><a></a><a></a><a></a><a></a><a></a><a>The development of highly-sensitive recognition of </a><a></a><a></a><a></a><a></a><a>hazardous </a>chemicals, such as volatile organic compounds (VOCs) and polycyclic aromatic hydrocarbons (PAHs), is of significant importance because of their widespread social concerns related to environment and human health. Here, we report a three-dimensional (3D) covalent organic framework (COF, termed JUC-555) bearing tetraphenylethylene (TPE) side chains as an aggregation-induced emission (AIE) fluorescence probe for sensitive molecular recognition.<a></a><a> </a>Due to the rotational restriction of TPE rotors in highly interpenetrated framework after inclusion of dimethylformamide (DMF), JUC-555 shows impressive AIE-based strong fluorescence. Meanwhile, owing to the large pore size (11.4 Å) and suitable intermolecular distance of aligned TPE (7.2 Å) in JUC-555, the obtained material demonstrates an excellent performance in the molecular recognition of hazardous chemicals, e.g., nitroaromatic explosives, PAHs, and even thiophene compounds, via a fluorescent quenching mechanism. The quenching constant (<i>K</i><sub>SV</sub>) is two orders of magnitude better than those of other fluorescence-based porous materials reported to date. This research thus opens 3D functionalized COFs as a promising identification tool for environmentally hazardous substances.</p>

Biomimetic Extracellular Vesicles Embedded with Black Phosphorus for Molecular Recognition-Guided Biomineralization

2018 ◽  
Author(s):  
Yingqian Wang ◽  
Xiaoxia Hu ◽  
Lingling Zhang ◽  
Chunli Zhu ◽  
Jie Wang ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Extracellular Vesicles ◽  
Inorganic Phosphate ◽  
Physiological Activity ◽  
Medical Engineering ◽  
Matrix Vesicles ◽  
Black Phosphorus ◽  
Photothermal Effect ◽  
Biological Processes ◽  
Extracellular Environment

Extracellular vesicles (EVs) are involved in the regulation of cell physiological activity and the reconstruction of extracellular environment. Matrix vesicles (MVs) are a type of EVs, and they participate in the regulation of cell mineralization. Herein, bioinspired MVs embedded with black phosphorus are functionalized with cell-specific aptamer (denoted as Apt-bioinspired MVs) for stimulating biomineralization. The aptamer can direct bioinspired MVs to targeted cells, and the increasing concentration of inorganic phosphate originated from the black phosphorus can facilitate cell biomineralization. The photothermal effect of the Apt-bioinspired MVs also positively affects mineralization. In addition, the Apt-bioinspired MVs display outstanding bone regeneration performance. Considering the excellent behavior of the Apt-bioinspired MVs for promoting biomineralization, our strategy provides a way of designing bionic tools for studying the mechanisms of biological processes and advancing the development of medical engineering.<br>

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