scholarly journals Genetic Diversity of the M RNA Segment among Crimean-Congo Hemorrhagic Fever Virus Isolates in China

Virology ◽  
2002 ◽  
Vol 296 (1) ◽  
pp. 159-164 ◽  
Author(s):  
Shigeru Morikawa ◽  
Tang Qing ◽  
Zhao Xinqin ◽  
Masayuki Saijo ◽  
Ichiro Kurane
Vestnik ◽  
2021 ◽  
pp. 323-329
Author(s):  
Г.Н. Абуова ◽  
Т.В. Полукчи ◽  
Ф.А. Бердалиева ◽  
Д.С. Алиев ◽  
Л.Л. Сарыпбекова ◽  
...  

Вирус Конго-Крымской геморрагической лихорадки является возбудителем тяжелой клещевой, часто смертельной, Конго-Крымской геморрагической лихорадки, которая широко распространена во всем мире. Передача вируса Конго-Крымской геморрагической лихорадки к человеку происходит через укус клеща, раздавливание зараженных клещей или контакт с инфицированной кровью человека. Миграция вируса способна через клещей с животными и перелетных птиц, в связи с чем имеется возможность исследования географического распространения посредством генетического анализа. В этом обзоре мы стремимся обобщить самые последние данные о генетическом разнообразии вирусов Конго-Крымской геморрагической лихорадки, циркулирующих в мире. Временной и пространственный анализ многообразия штаммов вируса может послужить ключевым фактором для лучшего понимания связи серопозитивности и уровня летальности. The Crimean-Congo hemorrhagic fever virus is the causative agent of severe tick-borne, often fatal, zoonotic Crimean-Congo hemorrhagic fever, which is widespread throughout the world. Transmission of the Crimean-Congo hemorrhagic fever virus to humans occurs through a tick bite, crushing of infected ticks or contact with infected host blood. The migration of the virus is possible through ticks with animals and migratory birds, and therefore it is possible to study the geographical distribution through genetic analysis. In this review, we aim to summarize the latest data on the genetic diversity of the Crimean-Congo hemorrhagic fever viruses circulating in the world. The temporal and spatial analysis of the diversity of virus strains can serve as a key factor for a better understanding of the relationship between seropositivity and the mortality rate.


2019 ◽  
Vol 65 (3) ◽  
pp. 451-461 ◽  
Author(s):  
Anne Rackow ◽  
Christa Ehmen ◽  
Ronald von Possel ◽  
Raquel Medialdea-Carrera ◽  
David Brown ◽  
...  

Abstract BACKGROUND The cellular surface molecule HsTOSO/FAIM3/HsFcμR has been identified as an IgM-specific Fc receptor expressed on lymphocytes. Here, we show that its extracellular immunoglobulin-like domain (HsFcμR-Igl) specifically binds to IgM/antigen immune complexes (ICs) and exploit this property for the development of novel detection systems for IgM antibodies directed against Crimean-Congo hemorrhagic fever virus (CCHFV) and Zika virus (ZIKV). METHODS His-tagged HsFcμR-Igl was expressed in Escherichia coli and purified by affinity chromatography, oxidative refolding, and size-exclusion chromatography. Specific binding of HsFcμR-Igl to IgM/antigen ICs was confirmed, and 2 prototypic ELISAs for the detection of anti-CCHFV and anti-ZIKV IgM antibodies were developed. Thereby, patient sera and virus-specific recombinant antigens directly labeled with horseradish peroxidase (HRP) were coincubated on HsFcμR-Igl-coated ELISA plates. Bound ICs were quantified by measuring turnover of a chromogenic HRP substrate. RESULTS Assay validation was performed using paired serum samples from 15 Kosovar patients with a PCR-confirmed CCHFV infection and 28 Brazilian patients with a PCR-confirmed ZIKV infection, along with a panel of a priori CCHFV/ZIKV-IgM-negative serum samples. Both ELISAs were highly reproducible. Sensitivity and specificity were comparable with or even exceeded in-house gold standard testing and commercial kits. Furthermore, latex beads coated with HsFcμR-Igl aggregated upon coincubation with an IgM-positive serum and HRP-labeled antigen but not with either component alone, revealing a potential for use of HsFcμR-Igl as a capture molecule in aggregation-based rapid tests. CONCLUSIONS Recombinant HsFcμR-Igl is a versatile capture molecule for IgM/antigen ICs of human and animal origin and can be applied for the development of both plate- and bead-based serological tests.


2021 ◽  
Vol 290 ◽  
pp. 114075
Author(s):  
Stephen Balinandi ◽  
Claudia von Brömssen ◽  
Alex Tumusiime ◽  
Jackson Kyondo ◽  
Hyesoo Kwon ◽  
...  

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