2010 ◽  
Vol 35 (2) ◽  
pp. 203-212 ◽  
Author(s):  
Mariusz Latos ◽  
Marek Pawełczyk

AbstractThere are many industrial environments which are exposed to a high-level noise, sometimes much higher than the level of speech. Verbal communication is then practically unfeasible. In order to increase the speech intelligibility, appropriate speech enhancement algorithms can be used. It is impossible to filter off the noise completely from the acquired signal by using a conventional filter, because of two reasons. First, the speech and the noise frequency contents are overlapping. Second, the noise properties are subject to change. The adaptive realisation of the Wiener-based approach can be, however, applied. Two structures are possible. One is the line enhancer, where the predictive realisation of the Wiener approach is used. The benefit of using this structure it that it does not require additional apparatus. The second structure takes advantage of the high level of noise. Under such condition, placing another microphone, even close to the primary one, can provide a reference signal well correlated with the noise disturbing the speech and lacking the information about the speech. Then, the classical Wiener filter can be used, to produce an estimate of the noise based on the reference signal. That noise estimate can be then subtracted from the disturbed speech. Both algorithms are verified, based on the data obtained from the real industrial environment. For laboratory experiments the G. R. A. S. artificial head and two microphones, one at back side of an earplug and another at the mouth are used.


Author(s):  
Joachim Frank

Cryo-electron microscopy combined with single-particle reconstruction techniques has allowed us to form a three-dimensional image of the Escherichia coli ribosome.In the interior, we observe strong density variations which may be attributed to the difference in scattering density between ribosomal RNA (rRNA) and protein. This identification can only be tentative, and lacks quantitation at this stage, because of the nature of image formation by bright field phase contrast. Apart from limiting the resolution, the contrast transfer function acts as a high-pass filter which produces edge enhancement effects that can explain at least part of the observed variations. As a step toward a more quantitative analysis, it is necessary to correct the transfer function in the low-spatial-frequency range. Unfortunately, it is in that range where Fourier components unrelated to elastic bright-field imaging are found, and a Wiener-filter type restoration would lead to incorrect results. Depending upon the thickness of the ice layer, a varying contribution to the Fourier components in the low-spatial-frequency range originates from an “inelastic dark field” image. The only prospect to obtain quantitatively interpretable images (i.e., which would allow discrimination between rRNA and protein by application of a density threshold set to the average RNA scattering density may therefore lie in the use of energy-filtering microscopes.


2020 ◽  
Vol 28 (3) ◽  
pp. 267-273
Author(s):  
E. S. Kaznacheeva ◽  
V. M. Kuz’kin ◽  
G. A. Lyakhov ◽  
S. A. Pereselkov ◽  
S. A. Tkachenko
Keyword(s):  

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