Production of Biopharmaceuticals Through Microbial Cell Immobilisation

2006 ◽  
pp. 407-422 ◽  
Author(s):  
Tajalli Keshavarz
Keyword(s):  
Microbial Cell ◽  
Cell Immobilisation

scholarly journals CapnocytophagacanimorsusMeningitis Diagnosed Using Next- Generation Sequencing of Microbial Cell-Free DNA

IDCases ◽  
2021 ◽  
pp. e01126
Author(s):  
Abuzar A. Asif ◽  
Moni Roy ◽  
Benjamin R. Tellier ◽  
Sharjeel Ahmad
Keyword(s):  
Next Generation Sequencing ◽  
Microbial Cell ◽  
Next Generation ◽  
Cell Free Dna ◽  
Free Dna ◽  
Generation Sequencing

scholarly journals 670. Rapid, Non-invasive Detection of Invasive Mycoplasma hominis Infection using the Karius Test, A Next-Generation Sequencing Test for Microbial Cell-free DNA in Plasma

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S390-S390
Author(s):  
Priya Edward ◽  
William V La Via ◽  
Mehreen Arshad ◽  
Kiran Gajurel
Keyword(s):  
Next Generation Sequencing ◽  
Case Series ◽  
Mycoplasma Hominis ◽  
Microbial Cell ◽  
Next Generation ◽  
Cell Free Dna ◽  
Non Invasive ◽  
Free Dna ◽  
Hominis Infection ◽  
Generation Sequencing

Abstract Background Mycoplasma hominis is typically associated with genital infections in women and is a rare cause of musculoskeletal infections often in immunocompromised hosts. Diagnosis of invasive Mycoplasma hominis infections are difficult due to challenges in culturing these organisms. Molecular diagnostics require an index of suspicion which may not be present at the time of tissue sampling. Accurate, rapid diagnosis of Mycoplasma hominis infections are important for antibiotic management. Methods Two cases of invasive Mycoplasma hominis infections are presented in which the Karius test (KT) was used to make the diagnosis. The KT is a CLIA certified/CAP-accredited next-generation sequencing (NGS) plasma test that detects microbial cell-free DNA (mcfDNA). After mcfDNA is extracted and NGS performed, human reads are removed and remaining sequences are aligned to a curated database of > 1400 organisms. Organisms present above a statistical threshold are reported. Case review was performed for clinical correlation. Results A young woman with lupus nephritis status post renal transplant developed persistent fever with progressive multifocal culture-negative osteoarticular infection despite empiric ceftriaxone. An adolescent female presented with an ascending pelvic infection progressing to purulent polymicrobial peritonitis (see table) requiring surgical debridement and cefipime, metronidazole and micafungin therapy; her course was complicated by progressive peritonitis/abscesses. Karius testing detected high-levels of Mycoplasma hominis mcfDNA in both cases – at 3251 molecules/microliter (MPM) in the first case and 3914 MPM in the second case. The normal range of Mycoplasma hominis mcfDNA in a cohort of 684 normal adults is 0 MPM. The patients rapidly improved with atypical coverage with doxycycline and levofloxaxin. Clinical findings in 2 patients with M. hominis infection detected by the Karius Test Conclusion Open-ended, plasma-based NGS for mcfDNA provides a rapid, non-invasive method to diagnose invasive Mycoplasma hominis infection. This case series highlights the potential to diagnose infections caused by fastidious pathogens to better inform antimicrobial therapy and achieve favorable outcomes. Disclosures William V. La Via, MD, Karius (Employee)

Developing fourth-generation biofuels secreting microbial cell factories for enhanced productivity and efficient product recovery; a review

Fuel ◽  
2021 ◽  
Vol 298 ◽  
pp. 120858
Author(s):  
Sana Malik ◽  
Ayesha Shahid ◽  
Chen-Guang Liu ◽  
Aqib Zafar Khan ◽  
Muhammad Zohaib Nawaz ◽  
...  
Keyword(s):  
Product Recovery ◽  
Microbial Cell ◽  
Fourth Generation ◽  
Microbial Cell Factories ◽  
Cell Factories

scholarly journals Immobilized microbial cell biocatalysts

2021 ◽  
Vol 14 (2) ◽  
pp. 752-753
Author(s):  
Lawrence P. Wackett
Keyword(s):  
Microbial Cell

Recent advances in engineering of microbial cell factories for intelligent pH regulation and tolerance

2021 ◽  
pp. 2100151
Author(s):  
Xiaopeng Gao ◽  
Ke Xu ◽  
Nadeem Ahmad ◽  
Lei Qin ◽  
Chun Li
Keyword(s):  
Ph Regulation ◽  
Microbial Cell ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Recent Advances

Multiple microbial cell-free extracts improve the microbiological, biochemical and sensory features of ewes’ milk cheese

2017 ◽  
Vol 66 ◽  
pp. 129-140 ◽  
Author(s):  
Maria Calasso ◽  
Leonardo Mancini ◽  
Maria De Angelis ◽  
Amalia Conte ◽  
Cristina Costa ◽  
...  
Keyword(s):  
Microbial Cell ◽  
Sensory Features

Needle microepiphytes in a Douglas fir canopy: biomass and distribution patterns

1979 ◽  
Vol 57 (9) ◽  
pp. 1000-1007 ◽  
Author(s):  
George C. Carroll
Keyword(s):  
Cell Volume ◽  
Distribution Patterns ◽  
Douglas Fir ◽  
Total Cell ◽  
Microbial Cell ◽  
Microbial Cells ◽  
Cutting Out ◽  
Total Cell Volume ◽  
Volume Estimates ◽  
Cell Volumes

Distribution patterns and total cell-volume estimates for needle microepiphytes are presented for three strata in the canopy of a single old-growth Douglas fir tree. Microbial cell volume was estimated by photographing transverse sections of needles, tracing microbial profiles on Mylar film, cutting out the tracings, and determining the pooled trace weights from various zones of each needle section. Microbial cells are concentrated in the midrib groove and over the stomatal zones of individual needles. Microbial cell volume on the upper needle surfaces increases during the 1st year and declines in subsequent years. Cell volumes on the lower needle surfaces increase from the 1st to the 3rd year and decrease from the 3rd to the 4th year. An increase in microbial cell volume occurs on both upper and lower surfaces from year 7 to year 8. Total microbial cell volume in relation to available needle surface area is greatest in the lower canopy and decreases with increasing height in the canopy. The total volume of microbial cells on needles was estimated to be 1093 cm3 for the entire tree.

Application of nanomaterials in microbial-cell biosensor constructions

2015 ◽  
Vol 69 (1) ◽  
Author(s):  
Jana Šefčovičová ◽  
Jan Tkac
Keyword(s):  
Carbon Nanotubes ◽  
Gold Nanoparticles ◽  
Rapid Detection ◽  
Food Industry ◽  
Low Cost ◽  
Microbial Cell ◽  
Direct Connection ◽  
Qualitative Detection ◽  
Analytical Tools ◽  
Cell Biosensor

AbstractMicrobial cell biosensors, where cells are in direct connection with a transducer enabling quantitative and qualitative detection of an analyte, are very promising analytical tools applied mainly for assays in the environmental field, food industry or biomedicine. Microbial cell biosensors are an excellent alternative to conventional analytical methods due to their specificity, rapid detection and low cost of analysis. Nowadays, nanomaterials are often used in the construction of biosensors to improve their sensitivity and stability. In this review, the combination of microbial and other individual cells with different nanomaterials (carbon nanotubes, graphene, gold nanoparticles, etc.) for the construction of biosensors is described and their applications are provided as well.

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