Working with multidetection in the analytical ultracentrifuge: the benefits of the combination of a refractive index detector and an absorption detector for the analysis of colloidal systems

2007 ◽  
pp. 121-128 ◽  
Author(s):  
A. Böhm ◽  
S. Kielhorn-Bayer ◽  
P. Rossmanith
Keyword(s):  
Refractive Index ◽  
Analytical Ultracentrifuge ◽  
Colloidal Systems ◽  
Refractive Index Detector

Development and Validation of a Novel Reversed Phase High Performance Liquid Chromatography with Refractive Index Detector Method for Assay of Polyvinyl Alcohol in an Ophthalmic Solution

2020 ◽  
Vol 16 (7) ◽  
pp. 867-871
Author(s):  
Harun Ergen ◽  
Muge Guleli ◽  
Cigdem Sener ◽  
Cem Caliskan ◽  
Sercan Semiz ◽  
...  
Keyword(s):  
Refractive Index ◽  
Liquid Chromatography ◽  
Polyvinyl Alcohol ◽  
High Performance ◽  
Ophthalmic Solution ◽  
Reversed Phase ◽  
Hplc Method ◽  
Solution Stability ◽  
Technical Requirements ◽  
Refractive Index Detector

Introduction: Polyvinyl alcohol (PVA), a polymer, is in demand due to its usage in different applications such as pharmaceutical, biomedical and textile, paper, food industries. Methods: A new sensitive reversed phased high-pressure liquid chromatography (RP-HPLC) method with refractive index detector (RID) was developed for determination of PVA in an ophthalmic solution containing dexpanthenol and PVA as active substances and it was validated according to The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) guideline. Results: Chromatographic separation was achieved on a Chiral-AGP (150 mm × 4.0 mm, 5 μm) column kept at 30°C with an isocratic flow at a flow rate of 1.0 ml/min. The detector temperature was 30°C, the retention time of PVA was around 1.0 min and the total run time was 5 minutes. Conclusion: The proposed method showed linearity, accuracy, precision, specificity, robustness, solution stability, and system suitability results within the acceptance criteria.

High Pressure Liquid Chromatographic Determination of Sorbitol in Bulk Sorbitol

1980 ◽  
Vol 63 (3) ◽  
pp. 664-666 ◽  
Author(s):  
J DOkladalova ◽  
A Y Barton ◽  
E A Mackenzie
Keyword(s):  
High Pressure ◽  
Refractive Index ◽  
Mobile Phase ◽  
Chromatographic Determination ◽  
High Pressure Liquid Chromatographic ◽  
Column Eluate ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Refractive Index Detector

Abstract An HPLC procedure for determining sorbitol in bulk sorbitol is described. An Aminex HPX-87 column (Bio-Rad) is used with water as a mobile phase and with a refractive index detector to monitor column eluate. Sorbitol is separated from pentaerythritol, erythritol, ribitol, ethylene glycol, propylene glycol, arabitol, galactitol, mannitol, iditol, and other carbohydrates. The precision of the sorbitol determination, characterized by a 95% confidence interval, corresponds to ±1.22%.

scholarly journals Individual and Total Sugar Contents of 83 Malaysian Foods

2018 ◽  
Vol 7 (3) ◽  
pp. 58 ◽  
Author(s):  
Norhayati Mustafa Khalid ◽  
Mohd Fairulnizal Md Noh ◽  
Mohd Naeem Mohd Nawi ◽  
Nazline Miasin Kehid ◽  
Aswir Abd Rashed ◽  
...  
Keyword(s):  
Refractive Index ◽  
Liquid Chromatography ◽  
Sugar Content ◽  
Meat Products ◽  
Food Products ◽  
Food Composition ◽  
Total Sugar ◽  
Cereal Products ◽  
Total Sugar Content ◽  
Refractive Index Detector

As part of the effort in updating and expanding the carbohydrate data in Malaysian Food Composition Databases, 83 foods were selected based on the most commonly consumed foods and food products by Malaysian. The samples include 31 cereal products, 9 starchy roots and tubers products, 4 legume products, 11 nut and seed products, 4 vegetables, 5 fruits, 15 sugar and syrup products, 2 meat products and 2 oil and fat products. Individual sugars (fructose, glucose, sucrose, lactose and maltose) were analysed usingHigh Performance Liquid Chromatography with Refractive Index Detector. Most of the cereal products contained sucrose, glucose, fructose, lactose and maltose. Four starchy root and tuber products contained sucrose, glucose and fructose. Sucrose was detected in all legume, nut and seed products. Most vegetables contained fructose while all fruits contained glucose and fructose. In addition, all syrups contained sucrose except for kiwi and lime cordial. Overall, sugar and syrup products contained the highest total sugar content (15.00-65.52 g/ 100g) while vegetables were the lowest for total sugar content (2.74-4.83 g/ 100g).

Determination of β-Fructofuranosidase by Liquid Chromatography with Refractive Index Detector

2013 ◽  
Vol 40 (10) ◽  
pp. 1602-1606 ◽  
Author(s):  
Yan WANG ◽  
Bin WU ◽  
Xiao-Yan ZHANG ◽  
Chong-Yu SHEN ◽  
Rui ZHANG ◽  
...  
Keyword(s):  
Refractive Index ◽  
Liquid Chromatography ◽  
Refractive Index Detector
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