Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions

2014 ◽  
pp. 65-71 ◽  
Author(s):  
Heba Badraiq ◽  
Liani Devito ◽  
Dusko Ilic
Keyword(s):  
Stem Cells ◽  
Umbilical Cord ◽  
Stromal Stem Cells

scholarly journals Isolation of mesenchymal stromal/stem cells from cryopreserved umbilical cord blood cells

2017 ◽  
Vol 57 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Sumie Fujii ◽  
Yasuo Miura ◽  
Masaki Iwasa ◽  
Satoshi Yoshioka ◽  
Aya Fujishiro ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells ◽  
Stromal Stem Cells

scholarly journals Differences in the intrinsic chondrogenic potential of equine umbilical cord matrix and cord blood mesenchymal stromal/stem cells for cartilage regeneration

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Rodolphe Rakic ◽  
Bastien Bourdon ◽  
Magali Demoor ◽  
Stéphane Maddens ◽  
Nathalie Saulnier ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Cartilage Regeneration ◽  
Chondrogenic Potential ◽  
Umbilical Cord Matrix ◽  
Stromal Stem Cells

scholarly journals Biological characteristics of stem cells derived from burned skin—a comparative study with umbilical cord stem cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Reinhard Dolp ◽  
Gertraud Eylert ◽  
Christopher Auger ◽  
Ayesha Aijaz ◽  
Yufei Andy Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Umbilical Cord ◽  
Mitochondrial Function ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Cell Secretion ◽  
Lineage Differentiation ◽  
Burned Skin ◽  
Mesenchymal Differentiation ◽  
Stromal Stem Cells

Abstract Introduction Burned human skin, which is routinely excised and discarded, contains viable mesenchymal stromal/stem cells (burn-derived mesenchymal stromal/stem cells; BD-MSCs). These cells show promising potential to enable and aid wound regeneration. However, little is known about their cell characteristics and biological function. Objectives This study had two aims: first, to assess critical and cellular characteristics of BD-MSCs and, second, to compare those results with multipotent well-characterized MSCs from Wharton’s jelly of human umbilical cords (umbilical cord mesenchymal stromal/stem cells, UC-MSCs). Methods BD- and UC-MSCs were compared using immunophenotyping, multi-lineage differentiation, seahorse analysis for glycolytic and mitochondrial function, immune surface markers, and cell secretion profile assays. Results When compared to UC-MSCs, BD-MSCs demonstrated a lower mesenchymal differentiation capacity and altered inflammatory cytokine secretomes at baseline and after stimulation with lipopolysaccharides. No significant differences were found in population doubling time, colony formation, cell proliferation cell cycle, production of reactive oxygen species, glycolytic and mitochondrial function, and in the expression of major histocompatibility complex I and II and toll-like receptor (TLR). Importance, translation This study reveals valuable insights about MSCs obtained from burned skin and show comparable cellular characteristics with UC-MSCs, highlighting their potentials in cell therapy and skin regeneration.

Umbilical cord‐derived mesenchymal stromal/stem cells expressing IL‐24 induce apoptosis in gliomas

2019 ◽  
Vol 235 (2) ◽  
pp. 1769-1779 ◽  
Author(s):  
Shaochen Fan ◽  
Huasong Gao ◽  
Wei Ji ◽  
Fengwei Zhu ◽  
Lingzheng Sun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Umbilical Cord ◽  
Stromal Stem Cells

Growth and spontaneous differentiation of umbilical-cord stromal stem cells on activated carbon cloth

2013 ◽  
Vol 1 (27) ◽  
pp. 3359 ◽  
Author(s):  
Virgínea de Araújo Farias ◽  
Jesús J. López-Peñalver ◽  
Julia Sirés-Campos ◽  
María Victoria López-Ramón ◽  
Carlos Moreno-Castilla ◽  
...  
Keyword(s):  
Stem Cells ◽  
Activated Carbon ◽  
Umbilical Cord ◽  
Carbon Cloth ◽  
Activated Carbon Cloth ◽  
Spontaneous Differentiation ◽  
Stromal Stem Cells

The importance of bystander effects in radiation therapy in melanoma skin-cancer cells and umbilical-cord stromal stem cells

2012 ◽  
Vol 102 (3) ◽  
pp. 450-458 ◽  
Author(s):  
Jaime Gómez-Millán ◽  
Iana Suly Santos Katz ◽  
Virgínea de Araujo Farias ◽  
Jose-Luis Linares-Fernández ◽  
Jesús López-Peñalver ◽  
...  
Keyword(s):  
Stem Cells ◽  
Radiation Therapy ◽  
Skin Cancer ◽  
Cancer Cells ◽  
Umbilical Cord ◽  
Bystander Effects ◽  
Stromal Stem Cells ◽  
Melanoma Skin

Isolation of mesenchymal stromal/stem cells from small-volume umbilical cord blood units that do not qualify for the banking system

2015 ◽  
Vol 102 (2) ◽  
pp. 218-229 ◽  
Author(s):  
Satoshi Yoshioka ◽  
Yasuo Miura ◽  
Masaki Iwasa ◽  
Aya Fujishiro ◽  
Hisayuki Yao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Small Volume ◽  
Banking System ◽  
Stromal Stem Cells

scholarly journals Publisher Correction: Differences in the intrinsic chondrogenic potential of equine umbilical cord matrix and cord blood mesenchymal stromal/stem cells for cartilage regeneration

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Rodolphe Rakic ◽  
Bastien Bourdon ◽  
Magali Demoor ◽  
Stéphane Maddens ◽  
Nathalie Saulnier ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Cartilage Regeneration ◽  
Chondrogenic Potential ◽  
Umbilical Cord Matrix ◽  
Stromal Stem Cells

scholarly journals Proliferative Effect of Aqueous Extract of Sea Cucumber (Holothuria Parva) Body Wall on Human Umbilical Cord Mesenchymal Stromal/stem Cells

2021 ◽  
Author(s):  
Poorya Rasekh ◽  
Ali Kameli ◽  
Arezoo Khoradmehr ◽  
Neda Baghban ◽  
Gholamhossein Mohebbi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Umbilical Cord ◽  
Aqueous Extract ◽  
Sea Cucumber ◽  
Body Wall ◽  
Control Group ◽  
Human Umbilical Cord ◽  
Stromal Stem Cells

Abstract Background: The sea cucumber potentials for stem cell proliferation induction and their mechanisms of bioactive compounds in its extract have been studied. Human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to aqueous extract of Holothuria parva body wall. Methods: Using GC-MS analysis on aqueous extract of H. parva, proliferative molecules were detected. The extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were used. MTT proliferation, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, effects of aqueous extract of H. parva and EGF on cell proliferation markers were detected. Computational modeling done to detect effective proliferative compounds in aqueous extract of H. parva. Results: MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of H. parva had proliferative effects on hUC-MSCs. Count of the cells treated with the 20 µg/mL of the extract was increased faster and higher than the control group (P<0.05). This concentration of extract did not have significant effects on hUC-MSCs viability. The cell cycle assay of hUC-MSCs showed that percent of cells in the G2 stage of the extract was biologically higher than the control group (P>0.05). Expression of the cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT were increased comparing with the control group. Moreover, expression of the p21 and PCNA decreased after treating hUC-MSCs with the extract. However, the CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of the cdk-4 and cdk-6 was decreased after treatment with the extract. Between the detected compounds, 1-methyl-4-(1-methylethenyl)-benzene showed better affinity to cdk-4 and p21 than tetradecanoic acid. Conclusions: The H. parva aqueous extract showed proliferative potential on the hUC-MSCs.

Functional comparison of beating cardiomyocytes differentiated from umbilical cord‐derived mesenchymal/stromal stem cells and human foreskin‐derived induced pluripotent stem cells

2019 ◽  
Vol 108 (3) ◽  
pp. 496-514 ◽  
Author(s):  
Pallavi Pushp ◽  
Bijayalaxmi Sahoo ◽  
Frederico C. Ferreira ◽  
Joaquim M. Sampaio Cabral ◽  
Ana Fernandes‐Platzgummer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Umbilical Cord ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent ◽  
Stromal Stem Cells
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