Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering

2017 ◽  
pp. 95-102 ◽  
Author(s):  
Matthew J. Hodgson ◽  
Christopher C. Knutson ◽  
Nima Momtahan ◽  
Alonzo D. Cook
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Porcine Heart

scholarly journals Recapitulating Cardiac Structure and Function In Vitro from Simple to Complex Engineering

Micromachines ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 386
Author(s):  
Ana Santos ◽  
Yongjun Jang ◽  
Inwoo Son ◽  
Jongseong Kim ◽  
Yongdoo Park
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Computational Modeling ◽  
Cardiac Tissue ◽  
Cardiac Development ◽  
Heart Tissue ◽  
Cardiac Tissue Engineering ◽  
Cardiac Cells

Cardiac tissue engineering aims to generate in vivo-like functional tissue for the study of cardiac development, homeostasis, and regeneration. Since the heart is composed of various types of cells and extracellular matrix with a specific microenvironment, the fabrication of cardiac tissue in vitro requires integrating technologies of cardiac cells, biomaterials, fabrication, and computational modeling to model the complexity of heart tissue. Here, we review the recent progress of engineering techniques from simple to complex for fabricating matured cardiac tissue in vitro. Advancements in cardiomyocytes, extracellular matrix, geometry, and computational modeling will be discussed based on a technology perspective and their use for preparation of functional cardiac tissue. Since the heart is a very complex system at multiscale levels, an understanding of each technique and their interactions would be highly beneficial to the development of a fully functional heart in cardiac tissue engineering.

scholarly journals Native cardiac environment and its impact on engineering cardiac tissue

2019 ◽  
Vol 7 (9) ◽  
pp. 3566-3580 ◽  
Author(s):  
Verena Schwach ◽  
Robert Passier
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Embryonic Development ◽  
Human Heart ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Adult Human ◽  
Natural And Synthetic

In this review, we describe the progressive build-up of the cardiac extracellular matrix (ECM) during embryonic development, the ECM of the adult human heart and the application of natural and synthetic biomaterials for cardiac tissue engineering using hPSC-CMs.

Vascularisation for cardiac tissue engineering: the extracellular matrix

2015 ◽  
Vol 113 (03) ◽  
pp. 532-547 ◽  
Author(s):  
Chinmoy Patra ◽  
Aldo Boccaccini ◽  
Felix Engel
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cardiac Tissue ◽  
Heart Diseases ◽  
Cardiac Tissue Engineering ◽  
Promising Candidate ◽  
Realistic Option ◽  
Extracellular Matrix Molecules ◽  
Heart Muscle Cells

SummaryCardiovascular diseases present a major socio-economic burden. One major problem underlying most cardiovascular and congenital heart diseases is the irreversible loss of contractile heart muscle cells, the cardiomyocytes. To reverse damage incurred by myocardial infarction or by surgical correction of cardiac malformations, the loss of cardiac tissue with a thickness of a few millimetres needs to be compensated. A promising approach to this issue is cardiac tissue engineering. In this review we focus on the problem of in vitro vascularisation as implantation of cardiac patches consisting of more than three layers of cardiomyocytes (> 100 μm thick) already results in necrosis. We explain the need for vascularisation and elaborate on the importance to include non-myocytes in order to generate functional vascularised cardiac tissue. We discuss the potential of extracellular matrix molecules in promoting vascularisation and introduce nephronectin as an example of a new promising candidate. Finally, we discuss current biomaterial- based approaches including micropatterning, electrospinning, 3D micro-manufacturing technology and porogens. Collectively, the current literature supports the notion that cardiac tissue engineering is a realistic option for future treatment of paediatric and adult patients with cardiac disease.

scholarly journals Protein/polysaccharide-based scaffolds mimicking native extracellular matrix for cardiac tissue engineering applications

2017 ◽  
Vol 106 (3) ◽  
pp. 769-781 ◽  
Author(s):  
Elisabetta Rosellini ◽  
Yu Shrike Zhang ◽  
Bianca Migliori ◽  
Niccoletta Barbani ◽  
Luigi Lazzeri ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Engineering Applications

Three-Dimensional Cardiac Tissue Engineering Using a Thermoresponsive Artificial Extracellular Matrix

ASAIO Journal ◽  
2004 ◽  
Vol 50 (4) ◽  
pp. 344-348 ◽  
Author(s):  
Hiroshi Naito ◽  
Yoshiaki Takewa ◽  
Toshihide Mizuno ◽  
Shoji Ohya ◽  
Yasuhide Nakayama ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cardiac Tissue ◽  
Three Dimensional ◽  
Cardiac Tissue Engineering ◽  
Artificial Extracellular Matrix

Mimicking Native Extracellular Matrix with Phytic Acid-Crosslinked Protein Nanofibers for Cardiac Tissue Engineering

2013 ◽  
Vol 13 (3) ◽  
pp. 366-375 ◽  
Author(s):  
Rajeswari Ravichandran ◽  
Vera Seitz ◽  
Jayarama Reddy Venugopal ◽  
Radhakrishnan Sridhar ◽  
Subramanian Sundarrajan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Phytic Acid ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering

scholarly journals Tunable Human Myocardium Derived Decellularized Extracellular Matrix for 3D Bioprinting and Cardiac Tissue Engineering

Gels ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 70
Author(s):  
Gozde Basara ◽  
S. Gulberk Ozcebe ◽  
Bradley W. Ellis ◽  
Pinar Zorlutuna
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Connexin 43 ◽  
Cardiac Tissue ◽  
Myocardial Infarct ◽  
Cardiac Tissue Engineering ◽  
3D Bioprinting ◽  
Decellularized Extracellular Matrix ◽  
Order Of Magnitude

The generation of 3D tissue constructs with multiple cell types and matching mechanical properties remains a challenge in cardiac tissue engineering. Recently, 3D bioprinting has become a powerful tool to achieve these goals. Decellularized extracellular matrix (dECM) is a common scaffold material due to providing a native biochemical environment. Unfortunately, dECM’s low mechanical stability prevents usage for bioprinting applications alone. In this study, we developed bioinks composed of decellularized human heart ECM (dhECM) with either gelatin methacryloyl (GelMA) or GelMA-methacrylated hyaluronic acid (MeHA) hydrogels dual crosslinked with UV light and microbial transglutaminase (mTGase). We characterized the bioinks’ mechanical, rheological, swelling, printability, and biocompatibility properties. Composite GelMA–MeHA–dhECM (GME) hydrogels demonstrated improved mechanical properties by an order of magnitude compared to the GelMA–dhECM (GE) hydrogels. All hydrogels were extrudable and compatible with human induced pluripotent stem cell derived cardiomyocytes (iCMs) and human cardiac fibroblasts (hCFs). Tissue-like beating of the printed constructs with striated sarcomeric alpha-actinin and connexin 43 expression was observed. The order of magnitude difference between the elastic modulus of these hydrogel composites offers applications in in vitro modeling of the myocardial infarct boundary. Here, as a proof of concept, we created an infarct boundary region with control over the mechanical properties along with the cellular and macromolecular content through printing iCMs with GE bioink and hCFs with GME bioink.

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