In Vitro Cellular Delivery of Peptide Nucleic Acid (PNA)

2020 ◽  
pp. 173-185
Author(s):  
Takehiko Shiraishi ◽  
Mahdi Ghavami ◽  
Peter E. Nielsen
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Cellular Delivery

Cellular Delivery and Antisense Effects of Peptide Nucleic Acid Conjugated to Polyethyleneimine via Disulfide Linkers

2010 ◽  
Vol 21 (10) ◽  
pp. 1933-1938 ◽  
Author(s):  
Peter R. Berthold ◽  
Takehiko Shiraishi ◽  
Peter E. Nielsen
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Cellular Delivery ◽  
Disulfide Linkers

Anti-Ki-67 peptide nucleic acid affects the proliferation and apoptosis of human renal carcinoma cells in vitro

Life Sciences ◽  
2005 ◽  
Vol 76 (16) ◽  
pp. 1873-1881 ◽  
Author(s):  
Jun-Nian Zheng ◽  
Ya-Feng Sun ◽  
Dong-Sheng Pei ◽  
Jun-Jie Liu ◽  
Xiao-Qing Sun ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Renal Carcinoma ◽  
Carcinoma Cells ◽  
Ki 67 ◽  
Proliferation And Apoptosis ◽  
Human Renal Carcinoma ◽  
Renal Carcinoma Cells

Cellular Delivery of Polyheteroaromate−Peptide Nucleic Acid Conjugates Mediated by Cationic Lipids

2006 ◽  
Vol 17 (1) ◽  
pp. 189-194 ◽  
Author(s):  
Takehiko Shiraishi ◽  
Nadia Bendifallah ◽  
Peter E. Nielsen
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Cationic Lipids ◽  
Cellular Delivery

Synthesis of α-(R)-/γ-(S)-Dimethyl Substituted Peptide Nucleic Acid Submonomer Using Mitsunobu Reaction

2019 ◽  
Vol 16 (5) ◽  
pp. 437-446
Author(s):  
Ahmed S. Abdelbaky ◽  
Ivan A. Prokhorov ◽  
Igor P. Smirnov ◽  
Kristina M. Koroleva ◽  
Vitaliy I. Shvets ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Solid Phase ◽  
Genetic Diseases ◽  
Mitsunobu Reaction ◽  
Phase Synthesis ◽  
Dna And Rna ◽  
Pna Synthesis ◽  
Biomedical Technologies

One of the major challenges facing modern biochemical and biomedical technologies are finding molecular tools for diagnosis and detection of genetic diseases. In this connection, several classes of oligonucleotides have been developed that can recognize and bind to DNA and RNA with high affinity and sequence selectivity and withstand enzymatic degradation by proteases and nucleases; however, few can traverse the cell membrane on their own. One such promising class of nucleic acid mimics developed in the last two decades which showed good results in vitro, are the peptide nucleic acids (PNAs). New chiral α- and γ-peptide Nucleic Acid (PNA) submonomer with methyl substituents in pseudopeptide backbone were synthesized via Mitsunobu reaction. The α-(R)-/γ-(S)-configuration of the chiral centres will ensure the preorganization of the PNA oligomer into a right-handed helix. The results obtained showed that Boc/Fmoc-submonomer compatible with Boc-protocol PNAs solid-phase synthesis on an MBHA resin. We synthesized simple and efficient α-R-, γ-S-disubstituted PNA submonomer based on L-Ala and D-Ala with the construction of the intermediate pseudopeptide moiety by Mitsunobu reaction for subsequent use in the Boc-Protocol of solid phase PNA synthesis.

Orexigenic and cytoprotective effects of a novel adenosine-enriched peptide nucleic acid compound: Implications for clinical application

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 14154-14154 ◽  
Author(s):  
J. T. D’Olimpio ◽  
S. Elliston ◽  
M. Dediego ◽  
R. Sodum
Keyword(s):  
Wound Healing ◽  
Nucleic Acid ◽  
Cell Lines ◽  
Receptor Binding ◽  
Adenosine Receptors ◽  
Peptide Nucleic Acid ◽  
Hek293 Cell ◽  
Smooth Muscle Relaxation ◽  
A2 Receptors

14154 Background: There is a major unmet need for new non-toxic orexigenic and cytoprotective agents that can ameliorate intractable symptoms in cancer such as anorexia, poor wound healing/ulcerations and poor QoL. Underlying causes of these clinical problems include excessive inflammatory/cytokine responses to either treatment related or tumor related processes, or to both at once. AVR118, a novel remarkably non-toxic peptide/nucleic acid formulation has been shown in many anecdotal reports and in small clinical trials, to stimulate appetite, improve fatigue, and assist in more rapidly reversing toxic side effects of chemotherapy, radiation and interferon therapy. It has now been re-characterized to better explain these multiple effects. It binds to adenosine receptors in vitro and increases cAMP release in HEK293 cell lines expressing A2 receptors, mechanistically involving targeted receptor binding, most likely in the gut and in the functional pathway of orexin A. Methodologies: HPLC, co-chromatography with standards, UV and mass spectrometry. Receptor binding/functional assays in vitro, animal models for anti-inflammatory and wound healing studies. Results: AVR 118 consists of a mix of 14 nucleoside derivatives, including available adenosine at a concentration of 2.1mM in stable solution. Also present is a complex aggregate of peptides showing high proline and tyrosine content. AVR 118 binds to adenosine receptors in vitro and increases cAMP release in HEK293 cell lines containing A2 receptors and enhances smooth muscle relaxation mediated by A2 and others such as NK2 and NTS1 in vitro. AVR 118 shows 20% enhancement of epithelialization when compared to saline controls in a controlled porcine wound healing model. Conclusions: AVR 118 may prove to play a major role in palliation of anorexia symptoms associated with serious debilitating diseases such as cancer or AIDS and as adjunctive therapy for these patients. [Table: see text] No significant financial relationships to disclose.

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