scholarly journals Correction to: Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos

2020 ◽  
pp. C1-C1
Author(s):  
Katalin Páldi ◽  
Magdalena Mosiolek ◽  
Michael D. Nodine
Keyword(s):  
Small Rna ◽  
In Situ Hybridizations

scholarly journals Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos

2020 ◽  
pp. 87-99
Author(s):  
Katalin Páldi ◽  
Magdalena Mosiolek ◽  
Michael D. Nodine
Keyword(s):  
In Situ Hybridization ◽  
Small Rna ◽  
Specific Cell ◽  
Posttranscriptional Gene Silencing ◽  
Regulatory Processes ◽  
Cellular Resolution ◽  
Gene Regulatory ◽  
Hybridization Protocol ◽  
In Situ Hybridizations

AbstractSmall RNAs mediate posttranscriptional gene silencing in plants and animals. This often occurs in specific cell or tissue types and can be necessary for their differentiation. Determining small RNA (sRNA) localization patterns at cellular resolution can therefore provide information on the corresponding gene regulatory processes they are involved in. Recent improvements with in situ hybridization methods have allowed them to be applied to sRNAs. Here we describe an in situ hybridization protocol to detect sRNAs from sections of early staged Arabidopsis thaliana (Arabidopsis) embryos.

scholarly journals Germline Transformation of Drosophila virilis With the Transposable Element mariner

Genetics ◽  
1996 ◽  
Vol 143 (1) ◽  
pp. 365-374 ◽  
Author(s):  
Allan R Lohe ◽  
Daniel L Hartl
Keyword(s):  
Transposable Element ◽  
Common Ancestor ◽  
Pcr Amplification ◽  
Drosophila Virilis ◽  
Cell Region ◽  
Diverse Species ◽  
Drosophila Mauritiana ◽  
Characteristic 2 ◽  
In Situ Hybridizations

Abstract An important goal in molecular genetics has been to identify a transposable element that might serve as an efficient transformation vector in diverse species of insects. The transposable element mariner occurs naturally in a wide variety of insects. Although virtually all mariner elements are nonfunctional, the Mosl element isolated from Drosophila mauritiana is functional. Mosl was injected into the pole-cell region of embryos of D. virilis, which last shared a common ancestor with D. mauritiana 40 million years ago. Mosl PCR fragments were detected in several pools of DNA from progeny of injected animals, and backcross lines were established. Because Go lines were pooled, possibly only one transformation event was actually obtained, yielding a minimum frequency of 4%. Mosl segregated in a Mendelian fashion, demonstrating chromosomal integration. The copy number increased by spontaneous mobilization. In situ hybridization confirmed multiple polymorphic locations of Mosl. Integration results in a characteristic 2-bp TA duplication. One Mosl element integrated into a tandem array of 370-bp repeats. Some copies may have integrated into heterochromatin, as evidenced by their ability to support PCR amplification despite absence of a signal in Southern and in situ hybridizations.

scholarly journals Small RNA Detection by in Situ Hybridization Methods

2015 ◽  
Vol 16 (12) ◽  
pp. 13259-13286 ◽  
Author(s):  
Martyna Urbanek ◽  
Anna Nawrocka ◽  
Wlodzimierz Krzyzosiak
Keyword(s):  
In Situ Hybridization ◽  
Small Rna ◽  
Rna Detection

Gelatin as a blocking agent in Southern blot and chromosomal in situ hybridizations

1993 ◽  
Vol 9 (8) ◽  
pp. 261-262 ◽  
Author(s):  
S.C. Lakhotia ◽  
Abbay Sharma ◽  
Mousumi Mutsuddi ◽  
Madhu G. Tapadia
Keyword(s):  
Southern Blot ◽  
Blocking Agent ◽  
In Situ Hybridizations

scholarly journals The Drosophila Embryo at Single Cell Transcriptome Resolution

2017 ◽  
Author(s):  
Nikos Karaiskos ◽  
Philipp Wahle ◽  
Jonathan Alles ◽  
Anastasiya Boltengagen ◽  
Salah Ayoub ◽  
...  
Keyword(s):  
Single Cell ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Drosophila Embryo ◽  
Evolutionary Changes ◽  
Cell Transcriptome ◽  
Mechanisms Of Development ◽  
In Situ Hybridizations ◽  
Single Cell Transcriptome

ABSTRACTDrosophila is a premier model system for understanding the molecular mechanisms of development. By the onset of morphogenesis, ~6000 cells express distinct gene combinations according to embryonic position. Despite extensive mRNA in situ screens, combinatorial gene expression within individual cells is largely unknown. Therefore, it is difficult to comprehensively identify the coding and non-coding transcripts that drive patterning and to decipher the molecular basis of cellular identity. Here, we single-cell sequence precisely staged embryos, measuring >3100 genes per cell. We produce a ‘transcriptomic blueprint’ of development – a virtual embryo where 3D locations of sequenced cells are confidently identified. Our “Drosophila-Virtual-Expression-eXplorer” performs virtual in situ hybridizations and computes expression gradients. Using DVEX, we predict spatial expression and discover patterned lncRNAs. DEVX is sensitive enough to detect subtle evolutionary changes in expression patterns between Drosophila species. We believe DVEX is a prototype for powerful single cell studies in complex tissues.

Expression of Drosophila epidermal growth factor receptor homologue in mitotic cell populations

Development ◽  
1987 ◽  
Vol 100 (2) ◽  
pp. 201-210 ◽  
Author(s):  
K.L. Kammermeyer ◽  
S.C. Wadsworth
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Mitotic Cell ◽  
Imaginal Discs ◽  
Cell Populations ◽  
Stage Of Development ◽  
Epidermal Growth ◽  
In Situ Hybridizations ◽  
Third Instar Larvae

Expression of the Drosophila homologue (DER) of the human epidermal growth factor (EGF) receptor has been studied during development by RNA blot hybridizations and in situ hybridizations. One of the hypothetical functions of the protein encoded by this gene is mitotic signal transduction and, therefore, we have searched for evidence of its expression in mitotic cell populations. Increased DER transcript levels were detected in virtually all cells in cellular blastoderm embryos, indicative of the onset of transcription of the gene at this stage of development. These transcripts persist at least until the formation of the ventral furrow at the beginning of gastrulation. Expression of DER has been investigated in populations of nondividing cells, imaginal cells and nervous tissue in third instar larvae. By RNA blot hybridizations, we have shown that imaginal discs contain the bulk of the DER transcripts present in the whole third instar larvae. This result has been confirmed by in situ hybridizations. All imaginal discs, representing dividing cells, contain DER transcripts while salivary glands, representing nondividing cells, do not. Within the central nervous system, hybridization of the DER probe is confined to small clusters of cells in the brain cortex. During oogenesis, cell division programs are highly ordered temporally and spatially. Follicle cells express high levels of DER RNA during their mitotic phase of growth but lose these transcripts as they cease division. Nurse cells also express DER transcripts at lower levels even though they are not dividing. However, nurse cell transcripts represent stored maternal RNA species destined for use during preblastoderm embryonic development. These results indicate that the DER gene is expressed in mitotic cell populations during several stages of development and is not expressed in certain populations of nonmitotic cells.

Sign in / Sign up

Export Citation Format

Share Document