High-Throughput Assessment of Changes in the Caenorhabditis elegans Gut Microbiome

2020 ◽  
pp. 131-144
Author(s):  
Fan Zhang ◽  
Jessica L. Weckhorst ◽  
Adrien Assié ◽  
Anastasia S. Khodakova ◽  
Mario Loeza-Cabrera ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Throughput ◽  
Gut Microbiome

High-throughput assessment of changes in the Caenorhabditis elegans gut microbiome v1 (protocols.io.rtzd6p6)

protocols.io ◽  
2018 ◽  
Author(s):  
Fan Zhang ◽  
Jessica Weckhorst ◽  
Adrien Assie ◽  
Anastasia Khodakova ◽  
Mario Loeza ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Throughput ◽  
Gut Microbiome

High-throughput expression, purification, and characterization of recombinant Caenorhabditis elegans proteins

2003 ◽  
Vol 307 (4) ◽  
pp. 928-934 ◽  
Author(s):  
Raymond Y Huang ◽  
Simon J Boulton ◽  
Marc Vidal ◽  
Steve C Almo ◽  
Anne R Bresnick ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Throughput ◽  
Purification And Characterization

scholarly journals High-Throughput Profiling of Caenorhabditis elegans Starvation-Responsive microRNAs

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142262 ◽  
Author(s):  
Laura Garcia-Segura ◽  
Cei Abreu-Goodger ◽  
Armando Hernandez-Mendoza ◽  
Tzvetanka D. Dimitrova Dinkova ◽  
Luis Padilla-Noriega ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Throughput

A novel high-throughput nematicidal assay using embryo cells and larvae of Caenorhabditis elegans

2014 ◽  
Vol 139 ◽  
pp. 33-41 ◽  
Author(s):  
Yiling Lai ◽  
Meichun Xiang ◽  
Shuchun Liu ◽  
Erwei Li ◽  
Yongsheng Che ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Throughput ◽  
Embryo Cells

scholarly journals CAENORHABDITIS ELEGANS AS A MODEL OF AIR POLLUTION TOXICITY DURING DEVELOPMENT AND LIFESPAN

2019 ◽  
Vol 3 (Supplement_1) ◽  
pp. S97-S97
Author(s):  
Amin Haghani ◽  
Hans M Dalton ◽  
Nikoo Safi ◽  
Farimah Shirmohammadi ◽  
Constantinos Sioutas ◽  
...  
Keyword(s):  
Air Pollution ◽  
Caenorhabditis Elegans ◽  
Mouse Models ◽  
High Throughput ◽  
Molecular Mechanisms ◽  
Cultured Cells ◽  
Exposure Model ◽  
C Elegans ◽  
Biological Responses ◽  
Short Term Exposure

Abstract Air pollution (AirPoll) is among the leading human mortality risk factors and yet little is known about the molecular mechanisms of this global environmental toxin. Our recent studies using mouse models even showed genetic variation and sex can alter biological responses to air pollution. To expand genetic studies of AirPoll toxicity throughout the lifespan, we introduced Caenorhabditis elegans as a new AirPoll exposure model which has a short lifespan, high throughput capabilities and shared longevity pathways with mammals. Acute exposure of C. elegans to airborne nanosized AirPoll matter (nPM) caused similar gene expression changes to our prior findings in cell culture and mouse models. Initial C. elegans responses to nPM included antioxidant, inflammatory and Alzheimer homolog genes. The magnitude of changes was dependent on the developmental stage of the worms. Even short term exposure of C. elegans to nPM altered developmental and lifespan hormetic effects, with pathways that included skn-1/Nrf family antioxidant responses. We propose C. elegans as a new and complementary model for mouse and cultured cells to study AirPoll across the lifespan. Future chronic nPM exposure and high throughput genetic screening of C. elegans can identify other major regulators of the developmental and lifespan effects of air pollution. This work was supported by grants R01AG051521 (CEF); R21AG05020 (CEF); Cure Alzheimer’s Fund (CEF); R01GM109028 (SPC), F31AG051382 (HMD) and T32AG000037 (HMD), T32AG052374 (AH).

scholarly journals Relationship between hyperlipidemia and the gut microbiome of rats, characterized using high-throughput sequencing

2020 ◽  
Vol 7 (2) ◽  
pp. 154-161 ◽  
Author(s):  
Yaqi Li ◽  
Quantao Ma ◽  
Jingkang Wang ◽  
Pengfei Li ◽  
Long Cheng ◽  
...  
Keyword(s):  
High Throughput ◽  
Gut Microbiome ◽  
High Throughput Sequencing

scholarly journals A high-throughput method for assessing chemical toxicity using a Caenorhabditis elegans reproduction assay

2010 ◽  
Vol 245 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Windy A. Boyd ◽  
Sandra J. McBride ◽  
Julie R. Rice ◽  
Daniel W. Snyder ◽  
Jonathan H. Freedman
Keyword(s):  
Caenorhabditis Elegans ◽  
High Throughput ◽  
Chemical Toxicity ◽  
High Throughput Method
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