A novel high-throughput nematicidal assay using embryo cells and larvae of Caenorhabditis elegans

2014 ◽  
Vol 139 ◽  
pp. 33-41 ◽  
Author(s):  
Yiling Lai ◽  
Meichun Xiang ◽  
Shuchun Liu ◽  
Erwei Li ◽  
Yongsheng Che ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Throughput ◽  
Embryo Cells

High-throughput expression, purification, and characterization of recombinant Caenorhabditis elegans proteins

2003 ◽  
Vol 307 (4) ◽  
pp. 928-934 ◽  
Author(s):  
Raymond Y Huang ◽  
Simon J Boulton ◽  
Marc Vidal ◽  
Steve C Almo ◽  
Anne R Bresnick ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Throughput ◽  
Purification And Characterization

scholarly journals High-Throughput Profiling of Caenorhabditis elegans Starvation-Responsive microRNAs

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142262 ◽  
Author(s):  
Laura Garcia-Segura ◽  
Cei Abreu-Goodger ◽  
Armando Hernandez-Mendoza ◽  
Tzvetanka D. Dimitrova Dinkova ◽  
Luis Padilla-Noriega ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Throughput

scholarly journals CAENORHABDITIS ELEGANS AS A MODEL OF AIR POLLUTION TOXICITY DURING DEVELOPMENT AND LIFESPAN

2019 ◽  
Vol 3 (Supplement_1) ◽  
pp. S97-S97
Author(s):  
Amin Haghani ◽  
Hans M Dalton ◽  
Nikoo Safi ◽  
Farimah Shirmohammadi ◽  
Constantinos Sioutas ◽  
...  
Keyword(s):  
Air Pollution ◽  
Caenorhabditis Elegans ◽  
Mouse Models ◽  
High Throughput ◽  
Molecular Mechanisms ◽  
Cultured Cells ◽  
Exposure Model ◽  
C Elegans ◽  
Biological Responses ◽  
Short Term Exposure

Abstract Air pollution (AirPoll) is among the leading human mortality risk factors and yet little is known about the molecular mechanisms of this global environmental toxin. Our recent studies using mouse models even showed genetic variation and sex can alter biological responses to air pollution. To expand genetic studies of AirPoll toxicity throughout the lifespan, we introduced Caenorhabditis elegans as a new AirPoll exposure model which has a short lifespan, high throughput capabilities and shared longevity pathways with mammals. Acute exposure of C. elegans to airborne nanosized AirPoll matter (nPM) caused similar gene expression changes to our prior findings in cell culture and mouse models. Initial C. elegans responses to nPM included antioxidant, inflammatory and Alzheimer homolog genes. The magnitude of changes was dependent on the developmental stage of the worms. Even short term exposure of C. elegans to nPM altered developmental and lifespan hormetic effects, with pathways that included skn-1/Nrf family antioxidant responses. We propose C. elegans as a new and complementary model for mouse and cultured cells to study AirPoll across the lifespan. Future chronic nPM exposure and high throughput genetic screening of C. elegans can identify other major regulators of the developmental and lifespan effects of air pollution. This work was supported by grants R01AG051521 (CEF); R21AG05020 (CEF); Cure Alzheimer’s Fund (CEF); R01GM109028 (SPC), F31AG051382 (HMD) and T32AG000037 (HMD), T32AG052374 (AH).

scholarly journals A high-throughput method for assessing chemical toxicity using a Caenorhabditis elegans reproduction assay

2010 ◽  
Vol 245 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Windy A. Boyd ◽  
Sandra J. McBride ◽  
Julie R. Rice ◽  
Daniel W. Snyder ◽  
Jonathan H. Freedman
Keyword(s):  
Caenorhabditis Elegans ◽  
High Throughput ◽  
Chemical Toxicity ◽  
High Throughput Method

Structural genomics for Caenorhabditis elegans: high throughput protein expression analysis

2004 ◽  
Vol 34 (1) ◽  
pp. 49-55 ◽  
Author(s):  
James B Finley ◽  
Shi-Hong Qiu ◽  
Chi-Hao Luan ◽  
Ming Luo
Keyword(s):  
Caenorhabditis Elegans ◽  
Protein Expression ◽  
Expression Analysis ◽  
High Throughput ◽  
Structural Genomics

DI-ICR-FT-MS-based high-throughput deep metabotyping: a case study of the Caenorhabditis elegans–Pseudomonas aeruginosa infection model

2014 ◽  
Vol 407 (4) ◽  
pp. 1059-1073 ◽  
Author(s):  
Michael Witting ◽  
Marianna Lucio ◽  
Dimitrios Tziotis ◽  
Brigitte Wägele ◽  
Karsten Suhre ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Caenorhabditis Elegans ◽  
High Throughput ◽  
Infection Model ◽  
Pseudomonas Aeruginosa Infection

scholarly journals High-throughput imaging of neuronal activity in Caenorhabditis elegans

2013 ◽  
Vol 110 (45) ◽  
pp. E4266-E4273 ◽  
Author(s):  
J. Larsch ◽  
D. Ventimiglia ◽  
C. I. Bargmann ◽  
D. R. Albrecht
Keyword(s):  
Caenorhabditis Elegans ◽  
Neuronal Activity ◽  
High Throughput ◽  
High Throughput Imaging

scholarly journals Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans

BMC Biology ◽  
2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Blanca Hernando-Rodríguez ◽  
Annmary Paul Erinjeri ◽  
María Jesús Rodríguez-Palero ◽  
Val Millar ◽  
Sara González-Hernández ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Image Analysis ◽  
Caenorhabditis Elegans ◽  
High Throughput ◽  
Essential Genes ◽  
Combined Flow
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