A Clonogenic Assay to Quantify Melanoma Micrometastases in Pulmonary Tissue

2021 ◽  
pp. 385-406
Author(s):  
Fabrizio Mattei ◽  
Sara Andreone ◽  
Giovanna Schiavoni
Keyword(s):  
Clonogenic Assay ◽  
Pulmonary Tissue

Relationship Between Mineral Fiber-Induced Pulmonary Tissue Reaction and MIP-2

1997 ◽  
Vol 9 (4) ◽  
pp. 650
Author(s):  
Kyoung Ah Kim ◽  
Young Lim ◽  
Dong Won Lee ◽  
Heung Nam Kim ◽  
Hwang Sin Chang ◽  
...  
Keyword(s):  
Tissue Reaction ◽  
Pulmonary Tissue ◽  
Mineral Fiber

Clonogenic Assay

BIO-PROTOCOL ◽  
2012 ◽  
Vol 2 (10) ◽  
Author(s):  
Xiaodong Yang
Keyword(s):  
Clonogenic Assay

A Tribute to Philip Marcus and the Development of the Clonogenic Assay

2015 ◽  
Vol 183 (5) ◽  
pp. 497-500
Author(s):  
Mary-Keara Boss ◽  
Mark Dewhirst
Keyword(s):  
Clonogenic Assay

Lung Decortication With Argon Plasma Energy for the Treatment of Chronic Pleural Empyema

2021 ◽  
pp. 155698452110111
Author(s):  
Savvas Lampridis ◽  
Sofoklis Mitsos ◽  
David R. Lawrence ◽  
Nikolaos Panagiotopoulos
Keyword(s):  
Energy Source ◽  
Surgical Management ◽  
Argon Plasma ◽  
Pleural Empyema ◽  
Controlled Trials ◽  
Air Leak ◽  
Pulmonary Tissue ◽  
Pure Argon ◽  
Plasma Energy ◽  
Minimal Depth

Lung decortication for the treatment of chronic pleural empyema remains a technically challenging procedure that is associated with bleeding and air leak. The recent advent of pure argon plasma has provided thoracic surgeons with an electrically neutral energy source for dissection and coagulation of pulmonary tissue with minimal depth of necrosis. In this article, we describe the technique of lung decortication with argon plasma energy (PlasmaJet, Plasma Surgical, Roswell, GA, USA) for the treatment of chronic pleural empyema. With appropriate application, the PlasmaJet can facilitate the removal of fibrous cortex with satisfactory hemostasis and aerostasis. Argon plasma energy can potentially be a useful adjunct in lung decortication. Controlled trials are needed to determine its role in the surgical management of advanced pleural empyema.

scholarly journals Renin Angiotensin Aldosterone System Antagonism in 2019 Novel Coronavirus Acute Lung Injury

2021 ◽  
Author(s):  
Davide Ventura ◽  
Amy L Carr ◽  
R Duane Davis ◽  
Scott Silvestry ◽  
Linda Bogar ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Angiotensin Ii Receptor ◽  
Pulmonary Tissue ◽  
Renin Angiotensin Aldosterone System ◽  
Angiotensin Converting Enzyme 2 ◽  
Renin Angiotensin ◽  
Receptor Blockers ◽  
Membrane Bound ◽  
Raas Inhibitors ◽  
Novel Coronavirus

Abstract It has been established SARS-CoV-2 uses angiotensin-converting enzyme 2 (ACE2), a membrane-bound regulatory peptide, for host cell entry. Renin-angiotensin-aldosterone system (RAAS) inhibitors have been reported to increase ACE2 in type 2 pneumocytes pulmonary tissue. Controversy exists for the continuation of ACE inhibitors, angiotensin II receptor blockers (ARBs), and mineralocorticoid receptor antagonists (MRAs) in the current pandemic. ACE2 serves as regulatory enzyme in maintaining homeostasis between proinflammatory Angiotensin II and anti-inflammatory Angiotensin 1,7 peptides. Derangements in these peptides are associated with cardiovascular disease and are implicated in the progression of acute respiratory distress syndrome (ARDS). Augmentation of the ACE2/Ang1,7 axis represent a critical target in the supportive management of COVID-19 associated lung disease. Observational data describing the use of RAAS inhibitors in the setting of SARS-CoV-2 have not borne signals of harm to date. However, equipoise persists requiring an analysis of novel agents including recombinant human-ACE2 and existing RAAS inhibitors while balancing ongoing controversies associated with increased coronavirus infectivity and virulence.

scholarly journals MF2C3: Multi-Feature Fuzzy Clustering to Enhance Cell Colony Detection in Automated Clonogenic Assay Evaluation

Symmetry ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 773 ◽  
Author(s):  
Carmelo Militello ◽  
Leonardo Rundo ◽  
Luigi Minafra ◽  
Francesco Paolo Cammarata ◽  
Marco Calvaruso ◽  
...  
Keyword(s):  
Clonogenic Assay ◽  
Ground Truth ◽  
General Purpose ◽  
Computational Method ◽  
Counting Procedure ◽  
Proliferative Effect ◽  
Biological Studies ◽  
Biological Technique ◽  
Fuzzy C Means Clustering ◽  
Cell Colony

A clonogenic assay is a biological technique for calculating the Surviving Fraction (SF) that quantifies the anti-proliferative effect of treatments on cell cultures: this evaluation is often performed via manual counting of cell colony-forming units. Unfortunately, this procedure is error-prone and strongly affected by operator dependence. Besides, conventional assessment does not deal with the colony size, which is generally correlated with the delivered radiation dose or administered cytotoxic agent. Relying upon the direct proportional relationship between the Area Covered by Colony (ACC) and the colony count and size, along with the growth rate, we propose MF2C3, a novel computational method leveraging spatial Fuzzy C-Means clustering on multiple local features (i.e., entropy and standard deviation extracted from the input color images acquired by a general-purpose flat-bed scanner) for ACC-based SF quantification, by considering only the covering percentage. To evaluate the accuracy of the proposed fully automatic approach, we compared the SFs obtained by MF2C3 against the conventional counting procedure on four different cell lines. The achieved results revealed a high correlation with the ground-truth measurements based on colony counting, by outperforming our previously validated method using local thresholding on L*u*v* color well images. In conclusion, the proposed multi-feature approach, which inherently leverages the concept of symmetry in the pixel local distributions, might be reliably used in biological studies.

scholarly journals Assessment of Aspergillus fumigatus in Guinea Pig Bronchoalveolar Lavages and Pulmonary Tissue by Culture and Realtime Polymerase Chain Reaction Studies

2012 ◽  
Vol 13 (1) ◽  
pp. 726-736
Author(s):  
Dennis G. Hooper ◽  
Vincent E. Bolton ◽  
John S. Sutton ◽  
Frederick T. Guilford ◽  
David C. Straus ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Guinea Pig ◽  
Aspergillus Fumigatus ◽  
Chain Reaction ◽  
Pulmonary Tissue ◽  
Polymerase Chain

scholarly journals Upregulation of pulmonary tissue factor, loss of thrombomodulin and immunothrombosis in SARS-CoV-2 infection

2021 ◽  
Vol 39 ◽  
pp. 101069
Author(s):  
Ivo M.B. Francischetti ◽  
Kevin Toomer ◽  
Yifan Zhang ◽  
Jayesh Jani ◽  
Zishan Siddiqui ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Pulmonary Tissue
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