scholarly journals Immobilization of Olfactory Receptors Carried by Nanosomes onto a Gold Sensor Surface

Author(s):  
Jasmina Vidic ◽  
Yanxia Hou
2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Aditya Nugraha ◽  
Masri Bin Ardin

PVDF sensor is a sensor that is often used to measure force, strain, vibration and heat. In this study, PVDF sensors with surface polarization are used to detect cutting forces on the machine. The PVDF sensor that has been polarized on the surface is placed in the chuck part of the engine. Measuring instrumen for testing and calibrating PVDF sensors is oscilloscope with increased loading and reduced axial and tangential directions. After the calibration process, the PVDF sensor was used to measure cutting force on drilling machine, and then the results were compared with the PCB piezotronics force sensor. The PVDF sensor output signal is measured and studied for its voltage using an oscilloscope, where the output signal is compared to the weight given to the PVDF sensor. From the results of these tests indicate that the maximum deviation in axial loading is 0.32V while the tangential loading is 0.31VKeywords. PVDF sensor, Surface polarization, Drilling machine, Cutting force


Author(s):  
AH Klein ◽  
CA Motti ◽  
AK Hillberg ◽  
T Ventura ◽  
P Thomas-Hall ◽  
...  

AbstractGastropod molluscs are among the most abundant species that inhabit coral reef ecosystems. Many are specialist predators, along with the giant triton snail Charonia tritonis (Linnaeus, 1758) whose diet consists of Acanthaster planci (crown-of-thorns starfish), a corallivore known to consume enormous quantities of reef-building coral. C. tritonis are considered vulnerable due to overexploitation, and a decline in their populations is believed to have contributed to recurring A. planci population outbreaks. Aquaculture is considered one approach that could help restore natural populations of C. tritonis and mitigate coral loss; however, numerous questions remain unanswered regarding their life cycle, including the molecular factors that regulate their reproduction and development. In this study, we have established a reference C. tritonis transcriptome derived from developmental stages (embryo and veliger) and adult tissues. This was used to identify genes associated with cell signalling, such as neuropeptides and G protein-coupled receptors (GPCRs), involved in endocrine and olfactory signalling. A comparison of developmental stages showed that several neuropeptide precursors are exclusively expressed in post-hatch veligers and functional analysis found that FFamide stimulated a significant (20.3%) increase in larval heart rate. GPCRs unique to veligers, and a diversity of rhodopsin-like GPCRs located within adult cephalic tentacles, all represent candidate olfactory receptors. In addition, the cytochrome P450 superfamily, which participates in the biosynthesis and degradation of steroid hormones and lipids, was also found to be expanded with at least 91 genes annotated, mostly in gill tissue. These findings further progress our understanding of C. tritonis with possible application in developing aquaculture methods.


Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4743
Author(s):  
Romain Duroux ◽  
Anne Mandeau ◽  
Gaelle Guiraudie-Capraz ◽  
Yannick Quesnel ◽  
Estelle Loing

Olfactory receptors (ORs) are expressed and active in various human tissues, including the skin. Although the sense of smell plays an important physiological role in the regulation of mood and stress, a link between olfactive compounds, ORs, and skin stress has yet to be established. This study aims to investigate the role of newly identified skin ORs and agonists in the modulation of skin stress. Screening for odorant molecules was done with cAMP functional assay to identify OR agonists. RT-qPCR and immunofluorescence microscopy were conducted to identify and quantify ORs in epidermal keratinocytes (NHEKs) and human skin explants, as well as to evaluate specific markers (G6PDH, loricrin, and γH2AX) of stress-induced skin alterations. A randomized double-blinded, split-face clinical study was performed on a panel of stressed women to measure the benefits of OR agonist treatment for skin. Three new ORs (OR10A6, OR2AG2, and OR11H4) were identified in skin. A specific Rose extract and its major constituent (phenylethyl alcohol) were found to activate these ORs. The extract composition was revealed by both GC/FID and GC/MS analyses simultaneously and showed the presence of 34 volatiles molecules. Moreover, epinephrine induces a skin stress response characterized by increased expression of G6PD, loricrin, and γH2AX biomarkers, and a decrease of OR expression. These effects were prevented in the presence of rose extract and its benefits were confirmed clinically by a decrease in the appearance of under-eye dark circles. Altogether, our findings suggest that ORs may represent a new, promising way to treat stress-associated skin disorders.


2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Hetan Chang ◽  
Yang Liu ◽  
Ting Yang ◽  
Paolo Pelosi ◽  
Shuanglin Dong ◽  
...  

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Marta Sanmartí-Espinal ◽  
Patrizia Iavicoli ◽  
Annalisa Calò ◽  
Marta Taulés ◽  
Roger Galve ◽  
...  
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