Biophysical and Structural Methods to Study the bHLHZip Region of Human c-MYC

Cells function through the complex temporal and spatial interplay of ions, metabolites, macromolecules and macromolecular assemblies. Biochemical approaches allow the investigator to define the components and the solution chemical reactions that might be involved in cellular functions. Static structural methods can yield information concerning the 2- and 3-D organization of known and unknown cellular constituents. Genetic and molecular techniques are powerful approaches that can alter specific functions through the manipulation of gene products and thus identify necessary components and sequences of molecular events. However, full knowledge of the mechanism of particular cell functions will require direct measurement of the interplay of cellular constituents. Therefore, there has been a need to develop methods that can yield chemical and molecular information in time and space in living cells, while allowing the integration of information from biochemical, molecular and genetic approaches at the cellular level.

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USING FUZZY CLUSTERING IN STRUCTURAL METHODS OF IMAGE CLASSIFICATION

Telecommunications and Radio Engineering ◽

10.1615/telecomradeng.v79.i9.50 ◽

2020 ◽

Vol 79 (9) ◽

pp. 781-791

Author(s):

V. О. Gorokhovatskyi ◽

I. S. Tvoroshenko ◽

N. V. Vlasenko

Keyword(s):

Image Classification ◽

Fuzzy Clustering ◽

Structural Methods

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POLIVARIABILITY OF THE BASTARD MOTIF IN THE SHORT STORIES BY GUY DE MAUPASSANT: CHARACTER IN SEARCH OF SELF-IDENTIFICATION

LITERARY PROCESS methodology names trends ◽

10.28925/2412-2475.2018.11.2027 ◽

2018 ◽

pp. 20-27

Author(s):

Oksana Galchuk

Keyword(s):

Short Stories ◽

Short Story ◽

Social Institutions ◽

Point Of View ◽

Guy De Maupassant ◽

Traditional Values ◽

The Social ◽

Role Of It ◽

Structural Methods

The theme of illegitimacy Guy de Maupassant evolved in his works this article perceives as one of the factors of the author’s concept of a person and the plane of intersection of the most typical motifs of his short stories. The study of the author’s concept of a person through the prism of polivariability of the motif of a bastard is relevant in today’s revision of traditional values, transformation of the usual social institutions and search for identities, etc. The purpose of the study is to give a definition to the existence specifics of the bastard motif in the Maupassant’s short stories by using historical and literary, comparative, structural methods of analysis as dominant. To do this, I analyze the content, variability and the role of this motive in the formation of the Maupassant’s concept of a person, the author’s innovations in its interpretation from the point of view of literary diachrony. Maupassant interprets the bastard motif in the social, psychological and metaphorical-symbolic sense. For the short stories with the presentation of this motif, I suggest the typology based on the role of it in the structure of the work and the ideological and thematic content: the short stories with a motif-fragment, the ones with the bastard’s leitmotif and the group where the bastard motif becomes a central theme. The Maupassant’s interpretation of the bastard motif combines the general tendencies of its existence in the world’s literary tradition and individual reading. The latter is the result of the author’s understanding of the relevant for the era issues: the transformation of the family model, the interest in the theory of heredity, the strengthening of atheistic sentiments, the growth of frustration in the system of traditional social and moral values etc. This study sets the ground for a prospective analysis of the evolution the bastard motif in the short-story collections of different years or a comparative study of the motif in short stories and novels by Maupassant.

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Quantum Structural Methods for the Solid State and Surfaces

10.21236/ada413986 ◽

1999 ◽

Author(s):

Frank Starrost ◽

Emily A. Carter

Keyword(s):

Solid State ◽

Structural Methods

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Hybrid structural methods to probe atomic features of the Type III Secretion Injectisome of Pathogenic Bacteria

Microscopy and Microanalysis ◽

10.1017/s1431927621003251 ◽

2021 ◽

Vol 27 (S1) ◽

pp. 826-827

Author(s):

Natalie Strynadka

Keyword(s):

Type Iii Secretion ◽

Pathogenic Bacteria ◽

Type Iii ◽

Structural Methods

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TEMA CINTA DALAM NOVEL SHIOSAI KARYA MISHIMA YUKIO

IZUMI ◽

10.14710/izumi.6.1.45-52 ◽

2017 ◽

Vol 6 (1) ◽

pp. 45

Author(s):

Zaki Ainul Fadli ◽

Nur Hastuti

Keyword(s):

Literary Work ◽

Major Theme ◽

Mishima Yukio ◽

Relationship Of ◽

Structural Methods

This paper is titled Theme of Love in Mishima Yukio’s Shiosai. The purpose of writing is to reveal the major themes in Mishima Yukio's Shiosai and its relationship to other structures, especially figures and characters, plots and backgrounds. The analysis uses structural methods that have the concept that in a building of a literary work consists of interrelated and inseparable structures. The results of this paper indicate that the theme of love is a major theme that is evidenced by the correlation and relationship of love problems with other structures, especially figures and characterizations, plot, and background.

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Edogawa Rampo’s short story Kagami Jigoku: A Structural Study

KIRYOKU ◽

10.14710/kiryoku.v4i1.7-17 ◽

2020 ◽

Vol 4 (1) ◽

pp. 7-17

Author(s):

Yuliani Rahmah

Keyword(s):

Short Stories ◽

Short Story ◽

Japanese Society ◽

Modern Technology ◽

Point Of View ◽

The Other ◽

Cultural Aspects ◽

Analysis Process ◽

Structural Methods ◽

Modern Era

The purpose of this research is to analyze the intrinsic elements found in the short story Kagami Jigoku by Edogawa Rampo. By using structural methods the analysis process find out the intrinsic elements which builds the Kagami Jikoku short story. As a result it is known that the Kagami Jikoku is a short story with a mystery theme as the hallmark of Rampo as its author. The characteristic of this short story can be seen from the theme which raised the unusual obsession problem of the main characters. With the first person point of view which tells in unusual way from the other short stories, the regression plot in Kagami Jikoku is able to tell the unique phenomenon of Japanese society and its modern technology through elements of place, time and socio-cultural aspects of Japanese society in the modern era

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Serum amyloid A forms stable oligomers that disrupt vesicles at lysosomal pH and contribute to the pathogenesis of reactive amyloidosis

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1707120114 ◽

2017 ◽

Vol 114 (32) ◽

pp. E6507-E6515 ◽

Cited By ~ 29

Author(s):

Shobini Jayaraman ◽

Donald L. Gantz ◽

Christian Haupt ◽

Olga Gursky

Keyword(s):

Serum Amyloid A ◽

Major Complication ◽

Lipid Vesicles ◽

Serum Amyloid ◽

High Density Lipoproteins ◽

Aa Amyloidosis ◽

Intrinsically Disordered ◽

Amyloid A ◽

Α Helix ◽

Structural Methods

Serum amyloid A (SAA) is an acute-phase plasma protein that functions in innate immunity and lipid homeostasis. SAA is a protein precursor of reactive AA amyloidosis, the major complication of chronic inflammation and one of the most common human systemic amyloid diseases worldwide. Most circulating SAA is protected from proteolysis and misfolding by binding to plasma high-density lipoproteins. However, unbound soluble SAA is intrinsically disordered and is either rapidly degraded or forms amyloid in a lysosome-initiated process. Although acidic pH promotes amyloid fibril formation by this and many other proteins, the molecular underpinnings are unclear. We used an array of spectroscopic, biochemical, and structural methods to uncover that at pH 3.5–4.5, murine SAA1 forms stable soluble oligomers that are maximally folded at pH 4.3 with ∼35% α-helix and are unusually resistant to proteolysis. In solution, these oligomers neither readily convert into mature fibrils nor bind lipid surfaces via their amphipathic α-helices in a manner typical of apolipoproteins. Rather, these oligomers undergo an α-helix to β-sheet conversion catalyzed by lipid vesicles and disrupt these vesicles, suggesting a membranolytic potential. Our results provide an explanation for the lysosomal origin of AA amyloidosis. They suggest that high structural stability and resistance to proteolysis of SAA oligomers at pH 3.5–4.5 help them escape lysosomal degradation, promote SAA accumulation in lysosomes, and ultimately damage cellular membranes and liberate intracellular amyloid. We posit that these soluble prefibrillar oligomers provide a missing link in our understanding of the development of AA amyloidosis.

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