Mass Spectrometry-Based Analytical Strategy for Single-Cell Proteomics

2021 ◽  
pp. 63-72
Author(s):  
Ákos Végvári
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Analytical Strategy

scholarly journals In Vivo Subcellular Mass Spectrometry Enables Proteo‐Metabolomic Single‐cell Systems Biology in a Chordate Embryo Developing to a Normally Behaving Tadpole (X. laevis)

2021 ◽  
Author(s):  
Camille Lombard-Banek ◽  
Jie Li ◽  
Erika P. Portero ◽  
Rosemary M. Onjiko ◽  
Chase D. Singer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Systems Biology ◽  
Single Cell ◽  
Cell Systems

Chemical Tagging Assisted Mass Spectrometry Analysis Enables Sensitive Determination of Phosphorylated Compounds in a Single Cell

2021 ◽  
Vol 93 (17) ◽  
pp. 6848-6856
Author(s):  
Fei-Long Liu ◽  
Tian-Tian Ye ◽  
Jiang-Hui Ding ◽  
Xiao-Ming Yin ◽  
Xiao-Ke Yang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Phosphorylated Compounds ◽  
Chemical Tagging ◽  
Sensitive Determination

scholarly journals Frontispiz: In Vivo Subcellular Mass Spectrometry Enables Proteo‐Metabolomic Single‐Cell Systems Biology in a Chordate Embryo Developing to a Normally Behaving Tadpole ( X. laevis )

2021 ◽  
Vol 133 (23) ◽  
Author(s):  
Camille Lombard‐Banek ◽  
Jie Li ◽  
Erika P. Portero ◽  
Rosemary M. Onjiko ◽  
Chase D. Singer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Systems Biology ◽  
Single Cell ◽  
Cell Systems

scholarly journals Discovery of Spatial Peptide Signatures for Neuroblastoma Risk Assessment by MALDI Mass Spectrometry Imaging

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3184
Author(s):  
Zhiyang Wu ◽  
Patrick Hundsdoerfer ◽  
Johannes H. Schulte ◽  
Kathy Astrahantseff ◽  
Senguel Boral ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Risk Assessment ◽  
Mass Spectrometry Imaging ◽  
Principal Component ◽  
Therapy Resistance ◽  
Risk Classification ◽  
Intratumor Heterogeneity ◽  
Ionization Mass ◽  
Characteristic Analysis ◽  
Analytical Strategy

Risk classification plays a crucial role in clinical management and therapy decisions in children with neuroblastoma. Risk assessment is currently based on patient criteria and molecular factors in single tumor biopsies at diagnosis. Growing evidence of extensive neuroblastoma intratumor heterogeneity drives the need for novel diagnostics to assess molecular profiles more comprehensively in spatial resolution to better predict risk for tumor progression and therapy resistance. We present a pilot study investigating the feasibility and potential of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to identify spatial peptide heterogeneity in neuroblastoma tissues of divergent current risk classification: high versus low/intermediate risk. Univariate (receiver operating characteristic analysis) and multivariate (segmentation, principal component analysis) statistical strategies identified spatially discriminative risk-associated MALDI-based peptide signatures. The AHNAK nucleoprotein and collapsin response mediator protein 1 (CRMP1) were identified as proteins associated with these peptide signatures, and their differential expression in the neuroblastomas of divergent risk was immunohistochemically validated. This proof-of-concept study demonstrates that MALDI-MSI combined with univariate and multivariate analysis strategies can identify spatially discriminative risk-associated peptide signatures in neuroblastoma tissues. These results suggest a promising new analytical strategy improving risk classification and providing new biological insights into neuroblastoma intratumor heterogeneity.

scholarly journals High yield accelerated reactions in nonvolatile microthin films: chemical derivatization for analysis of single-cell intracellular fluid

2018 ◽  
Vol 9 (40) ◽  
pp. 7779-7786 ◽  
Author(s):  
Zhenwei Wei ◽  
Xiaochao Zhang ◽  
Jinyu Wang ◽  
Sichun Zhang ◽  
Xinrong Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Individual Cell ◽  
High Yield ◽  
Chemical Derivatization ◽  
Intracellular Fluid ◽  
Mild Conditions ◽  
Successful Analysis ◽  
Trace Components

The identification of trace components from an individual cell can require derivatization under mild conditions for successful analysis by mass spectrometry (MS).

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