Effects of Fibrinogen and Associated Peptide Fragments on the Activation of Human Endothelial Cells by VEGF in Vitro

2004 ◽  
pp. 117-132
Author(s):  
Carolyn A. Staton ◽  
Nicola J. Brown ◽  
Claire E. Lewis
Keyword(s):  
Endothelial Cells ◽  
Peptide Fragments ◽  
Human Endothelial Cells

Vascular repair utilising immobilised heparin conjugate for protection against early activation of inflammation and coagulation

2015 ◽  
Vol 113 (06) ◽  
pp. 1312-1322 ◽  
Author(s):  
Sofia Nordling ◽  
Jaan Hong ◽  
Karin Fromell ◽  
Fredrik Edin ◽  
Johan Brännström ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Thrombus Formation ◽  
Innate Immune ◽  
Human Endothelial Cells ◽  
Coagulation Activation ◽  
Ischaemia Reperfusion Injury ◽  
Novel Approach ◽  
One Step ◽  
Chamber Model

SummaryIschaemia-reperfusion injury (IRI) poses a major challenge in many thrombotic conditions and in whole organ transplantation. Activation of the endothelial cells and shedding of the protective vascular glycocalyx during IRI increase the risk of innate immune activation, cell infiltration and severe thrombus formation, promoting damage to the tissue. Here, we present a novel one-step strategy to protect the vasculature by immobilisation of a unique multi-arm heparin conjugate to the endothelium. Applying a new in vitro blood endothelial cell chamber model, the heparin conjugate was found to bind not only to primary human endothelial cells but also directly to the collagen to which the cells adhered. Incubation of hypoxic endothelial cells with freshly drawn human blood in the blood chambers elicited coagulation activation reflected by thrombin anti-thrombin formation and binding of platelets and neutrophils. Immobilisation of the heparin conjugate to the hypoxic endothelial cells created a protective coating, leading to a significant reduction of the recruitment of blood cells and coagulation activation compared to untreated hypoxic endothelial cells. This novel approach of immobilising multi-arm heparin conjugates on the endothelial cells and collagen of the basement membrane ensures to protect the endothelium against IRI in thrombotic disorders and in transplantation.

Centrifugal enhancement of Kaposi's sarcoma-associated virus infection of human endothelial cells in vitro

2008 ◽  
Vol 154 (1-2) ◽  
pp. 160-166 ◽  
Author(s):  
Seung-Min Yoo ◽  
Ae-Kyung Ahn ◽  
Taegun Seo ◽  
Hyo Bong Hong ◽  
Myung-Ae Chung ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Virus Infection ◽  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Human Endothelial Cells

Interactions of Haemophilus influenzae with Human Endothelial Cells In Vitro

1992 ◽  
Vol 165 (Supplement 1) ◽  
pp. S115-S116 ◽  
Author(s):  
M. Virji ◽  
H. Kayhty ◽  
D. J. P. Ferguson ◽  
C. Alexandrescu ◽  
E. R. Moxon
Keyword(s):  
Endothelial Cells ◽  
Haemophilus Influenzae ◽  
Human Endothelial Cells

β2-adrenergic agonist protects human endothelial cells from hypoxia/reoxygenation injury in vitro

2006 ◽  
Vol 34 (1) ◽  
pp. 165-172 ◽  
Author(s):  
Julien Pottecher ◽  
Gaëlle Cheisson ◽  
Olivier Huet ◽  
Christian Laplace ◽  
Eric Vicaut ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Endothelial Cells ◽  
Adrenergic Agonist ◽  
Reoxygenation Injury ◽  
Hypoxia Reoxygenation

Two different laminin domains mediate the differentiation of human endothelial cells into capillary-like structures in vitro

Cell ◽  
1989 ◽  
Vol 58 (5) ◽  
pp. 933-943 ◽  
Author(s):  
Derrick S. Grant ◽  
Ken-Ichiro Tashiro ◽  
Bartolome Segui-Real ◽  
Yoshihiko Yamada ◽  
George R. Martin ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Endothelial Cells

Replicative senescence of human endothelial cells in vitro involves G1 arrest, polyploidization and senescence-associated apoptosis

2001 ◽  
Vol 36 (8) ◽  
pp. 1327-1347 ◽  
Author(s):  
Mechthild Wagner ◽  
Barbara Hampel ◽  
David Bernhard ◽  
Monika Hala ◽  
Werner Zwerschke ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Replicative Senescence ◽  
G1 Arrest ◽  
Human Endothelial Cells
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