The Industrial Production of Monoclonal Antibodies in Cell Culture

1987 ◽  
pp. 97-109 ◽  
Author(s):  
J. R. Birch ◽  
K. Lambert ◽  
R. Boraston ◽  
P. W. Thompson ◽  
S. Garland ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Industrial Production

Interaction of mammalian cell culture broth with adsorbents in expanded bed adsorption of monoclonal antibodies

1999 ◽  
Vol 34 (2) ◽  
pp. 159-165 ◽  
Author(s):  
J. Feuser ◽  
M. Halfar ◽  
D. Lütkemeyer ◽  
N. Ameskamp ◽  
M.-R. Kula ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Culture Broth ◽  
Expanded Bed Adsorption ◽  
Expanded Bed

Development of a novel ELISA for human insulin using monoclonal antibodies produced in serum-free cell culture medium

2007 ◽  
Vol 40 (1-2) ◽  
pp. 98-103 ◽  
Author(s):  
Megha S. Even ◽  
Chad B. Sandusky ◽  
Neal D. Barnard ◽  
Jehangir Mistry ◽  
Madhur K. Sinha
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Human Insulin ◽  
Culture Medium ◽  
Free Cell ◽  
Cell Culture Medium ◽  
Serum Free

scholarly journals Analysis of Defined Combinations of Monoclonal Antibodies in Anthrax Toxin Neutralization Assays and Their Synergistic Action

2012 ◽  
Vol 19 (5) ◽  
pp. 731-739 ◽  
Author(s):  
Miriam M. Ngundi ◽  
Bruce D. Meade ◽  
Stephen F. Little ◽  
Conrad P. Quinn ◽  
Cindi R. Corbett ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Protective Antigen ◽  
Neutralizing Antibody ◽  
Synergistic Action ◽  
Anthrax Toxin ◽  
Content Type ◽  
Antagonistic Effects ◽  
Antibody Interactions ◽  
New Vaccines

ABSTRACTAntibodies against the protective antigen (PA) component of anthrax toxin play an important role in protection against disease caused byBacillus anthracis. In this study, we examined defined combinations of PA-specific monoclonal antibodies for their ability to neutralize anthrax toxin in cell culture assays. We observed additive, synergistic, and antagonistic effects of the antibodies depending on the specific antibody combination examined and the specific assay used. Synergistic toxin-neutralizing antibody interactions were examined in more detail. We found that one mechanism that can lead to antibody synergy is the bridging of PA monomers by one antibody, with resultant bivalent binding of the second antibody. These results may aid in optimal design of new vaccines and antibody therapies against anthrax.

scholarly journals Sustainable strategies based on glycine–betaine analogue ionic liquids for the recovery of monoclonal antibodies from cell culture supernatants

2019 ◽  
Vol 21 (20) ◽  
pp. 5671-5682 ◽  
Author(s):  
Emanuel V. Capela ◽  
Alexandre E. Santiago ◽  
Ana F. C. S. Rufino ◽  
Ana P. M. Tavares ◽  
Matheus M. Pereira ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Downstream Processing ◽  
Aqueous Biphasic Systems ◽  
Phase Partitioning ◽  
Hybrid Processes ◽  
Three Phase ◽  
Aqueous Biphasic ◽  
Biphasic Systems ◽  
Culture Supernatants

Ionic-liquid-based aqueous biphasic systems, three phase partitioning methods and hybrid processes combined with ultrafiltration are sustainable strategies for the downstream processing of monoclonal antibodies.

Sign in / Sign up

Export Citation Format

Share Document