Cloning of an Enzymatically Active Segment of the Exotoxin-A Gene of Pseudomonas Aeruginosa

1985 ◽  
pp. 777-790
Author(s):  
Royston C. Clowes ◽  
Mark A. Mozola ◽  
Russell B. Wilson ◽  
Shin R. Hwang ◽  
Rockford K. Draper
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Exotoxin A ◽  
Active Segment

scholarly journals Isolation and characterization of Pseudomonas aeruginosa exotoxin A binding glycoprotein from mouse LM cells

1991 ◽  
Vol 266 (4) ◽  
pp. 2390-2396 ◽  
Author(s):  
M R Thompson ◽  
J Forristal ◽  
P Kauffmann ◽  
T Madden ◽  
K Kozak ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Exotoxin A ◽  
Isolation And Characterization ◽  
Pseudomonas Aeruginosa Exotoxin ◽  
Binding Glycoprotein

scholarly journals Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zahra Shadman ◽  
Safar Farajnia ◽  
Mohammad Pazhang ◽  
Mohammadreza Tohidkia ◽  
Leila Rahbarnia ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Factors ◽  
High Reactivity ◽  
Scfv Antibody ◽  
Phage Library ◽  
Human Antibody ◽  
Exotoxin A ◽  
Phage Elisa ◽  
Human Scfv ◽  
Domain I

Abstract Background Pseudomonas aeruginosa is the leading cause of nosocomial infections, especially in people with a compromised immune system. Targeting virulence factors by neutralizing antibodies is a novel paradigm for the treatment of antibiotic-resistant pseudomonas infections. In this respect, exotoxin A is one of the most potent virulence factors in P. aeruginosa. The present study was carried out to identify a novel human scFv antibody against the P. aeruginosa exotoxin A domain I (ExoA-DI) from a human scFv phage library. Methods The recombinant ExoA-DI of P. aeruginosa was expressed in E. coli, purified by Ni-NTA column, and used for screening of human antibody phage library. A novel screening procedure was conducted to prevent the elimination of rare specific clones. The phage clone with high reactivity was evaluated by ELISA and western blot. Results Based on the results of polyclonal phage ELISA, the fifth round of biopanning leads to the isolation of several ExoA-DI reactive clones. One positive clone with high affinity was selected by monoclonal phage ELISA and used for antibody expression. The purified scFv showed high reactivity with the recombinant domain I and full-length native exotoxin A. Conclusions The purified anti-exotoxin A scFv displayed high specificity against exotoxin A. The human scFv identified in this study could be the groundwork for developing a novel therapeutic agent to control P. aeruginosa infections.

The frequency of genes encoding exotoxin A and exoenzyme S in Pseudomonas aeruginosa strains isolated from burn patients

Burns ◽  
2016 ◽  
Vol 42 (5) ◽  
pp. 1116-1120 ◽  
Author(s):  
Azar Dokht Khosravi ◽  
Fatemeh Shafie ◽  
Effat Abbasi Montazeri ◽  
Soodabeh Rostami
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Burn Patients ◽  
Exotoxin A ◽  
Exoenzyme S ◽  
Genes Encoding

Isolation and characterization of a Pseudomonas aeruginosa gene, ptxR , which positively regulates exotoxin A production

1996 ◽  
Vol 21 (1) ◽  
pp. 97-110 ◽  
Author(s):  
Abdul N. Hamood ◽  
Jane A. Colmer ◽  
Urs A. Ochsner ◽  
Michael L. Vasil
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Exotoxin A ◽  
Isolation And Characterization
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