Reversal of Enzymatic Profiles and Capillary Supply of Muscle Fibers in Fast and Slow Muscles After Cross Innervation

Author(s):  
Flaviu C. A. Romanul
1983 ◽  
Vol 61 (12) ◽  
pp. 1516-1522 ◽  
Author(s):  
K. Przyklenk ◽  
A. C. Groom

Muscle fibers in the transition zone of an infarcted heart are thought to be potentially ischemic during the first 6–9 h following coronary artery occlusion. However, the long-term fate of the muscle fibers at the margin of the necrosis is uncertain. Ischemia implies reduced oxygenation, possibly owing to a reduced capillary supply; thus our objective was to determine whether a region of reduced microvascular supply exists at the margin of a necrosis produced by chronic coronary artery occlusion. Five variables were used to quantitate the capillary supply in the transition zone: C/F (capillary to fiber) ratio, Vf (number of vessels around a fiber), Fv (number of fibers surrounding a vessel), capillary density, and fiber diameter. Infarcts were induced in young male rats by ligating the left coronary artery midway between its origin and the apex of the heart. Five weeks later, the capillary supply in the transition zone was significantly below control values, i.e., significant reductions in C/F, Vf, and Fv were found. This region of reduced capillary supply extended 225–525 μm laterally from the edge of the necrosis. Thus, a narrow transition zone, defined as a region of viable muscle fibers with a subnormal microvascular supply, exists as long as 5 weeks following coronary artery occlusion in the rat.


1937 ◽  
Vol 65 (1) ◽  
pp. 29-42 ◽  
Author(s):  
R. A. Shipley ◽  
Louise J. Shipley ◽  
Joseph T. Wearn

1. During normal growth of the rabbit heart, muscle fibers enlarge, and the capillaries multiply so that a relatively constant capillary supply per unit of tissue is maintained from the time of birth to maturity. 2. In cardiac hypertrophy the muscle fibers enlarge, but the capillaries do not multiply and, as a result, the capillary supply per unit of tissue is reduced. 3. The decreased concentration of capillaries in the hypertrophied heart would constitute an impediment to the adequate exchange of metabolic substances, but the seriousness of the impediment cannot be estimated without further physiological data.


1995 ◽  
Vol 24 (2) ◽  
pp. 87-89 ◽  
Author(s):  
E. Luque ◽  
J. Peña ◽  
P. Martin ◽  
I. Jimena ◽  
R. Vaamonde

Author(s):  
F. G. Zaki

Choline-deficiency was induced in Holtzman young rats of both sexes by feeding them a high fat - low protein diet.Preliminary studies of the ultrastructural changes in the myocardium of these animals have been recently reported from this laboratory. Myocardial lesions first appeared in the form of intraventricular mural thrombi, loss of cross striation of muscle fibers and focal necrosis of muscle cells associated with interstitial myocarditis. Prolonged choline-deficiency induced cardiomegaly associated with pericardial edema.During the early phase of this nutritional disorder, heart mitochondria - despite of not showing any swelling similar to that usually encountered in liver mitochondria of the same animal - ware the most ubiquitous site of marked structural abnormalities. Early changes in mitochondria appeared as vacuolation, disorganization, disruption and loss of cristae. Degenerating mitochondria were often seen quite enlarged and their matrix was replaced by whorls of myelin figures resembling lysosomal structures especially where muscle fibers were undergoing necrosis. In some areas, mitochondria appeared to be unusually clumped together where some contained membranelined vacuoles and others enclosed dense bodies and granular inclusions.


Author(s):  
I. Taylor ◽  
P. Ingram ◽  
J.R. Sommer

In studying quick-frozen single intact skeletal muscle fibers for structural and microchemical alterations that occur milliseconds, and fractions thereof, after electrical stimulation, we have developed a method to compare, directly, ice crystal formation in freeze-substituted thin sections adjacent to all, and beneath the last, freeze-dried cryosections. We have observed images in the cryosections that to our knowledge have not been published heretofore (Figs.1-4). The main features are that isolated, sometimes large regions of the sections appear hazy and have much less contrast than adjacent regions. Sometimes within the hazy regions there are smaller areas that appear crinkled and have much more contrast. We have also observed that while the hazy areas remain still, the regions of higher contrast visibly contract in the beam, often causing tears in the sections that are clearly not caused by ice crystals (Fig.3, arrows).


2020 ◽  
pp. 75-77
Author(s):  
Ekaterina Sergeevna Krasnikova ◽  
Natalya Lvovna Morgunova ◽  
Phelix Yakovlevich Rudik ◽  
Aleksandr Vladimirovich Krasnikov ◽  
Nikita Andreevich Semilet

The results of a study of the effect of wet ultrasonic lamb meat salting on the muscle tissue microstructure are presented, and the technical parameters of the ultrasonic device are justified. It has been established that significant destruction and swelling of muscle fibers, local destruction of the sarcolemma with its pronounced rugosity are observed at ultrasonic salting with a frequency of 35 kHz, the cross-striation is poorly expressed, the tissue structure is disturbed. An ultrasonic salting with a frequency of 26 kHz was accompanied an increase in the number of transverse microcracks and crevices, loosening of muscle fibers, the formation of cavities between them while retention of the tissue structure, which contributes to the appearance of a brine between muscle fibers and accelerates its penetration into the fiber. It allows us to recommend ultrasound at a frequency of 26 kHz for cavitation activation of the brine to intensify the technological process of lamb meat salting.


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