Hypertension Differentially Affects the Expression of the Gap Junction Protein Connexin43 in Cardiac Myocytes and Aortic Smooth Muscle Cells

1997 ◽  
pp. 71-82 ◽  
Author(s):  
Jacques-Antoine Haefliger ◽  
Einar Castillo ◽  
Gérard Waeber ◽  
Jean-François Aubert ◽  
Pascal Nicod ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Gap Junction ◽  
Cardiac Myocytes ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Junction Protein ◽  
Gap Junction Protein

scholarly journals Reciprocal expression of connexin 40 and 45 during phenotypical changes in renin-secreting cells

2011 ◽  
Vol 300 (3) ◽  
pp. F743-F748 ◽  
Author(s):  
Birguel Kurt ◽  
Lisa Kurtz ◽  
Maria L. Sequeira-Lopez ◽  
R. Ariel Gomez ◽  
Klaus Willecke ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Gap Junction ◽  
Vascular Smooth Muscle Cells ◽  
Cell Lineage ◽  
Muscle Cells ◽  
Sodium Deficiency ◽  
Junction Protein ◽  
Gap Junction Protein

Gap junctional coupling of renin-producing cells is of major functional importance for the control of renin synthesis and release. This study was designed to determine the relevance of the vascular gap junction protein connexin 45 (Cx45) for the control of renin expression and secretion. By crossbreeding mice which drive Cre recombinase under the control of the endogenous renin promoter with mice harboring floxed Cx45 gene alleles, we generated viable mice with a deletion of Cx45 in the renin cell lineage. These mice were normotensive, and renin cells in their kidneys were normal with regard to localization and number. Sodium deficiency induced typical recruitment of renin-producing cells along afferent arterioles, whereas sodium overload resulted in a decrease in the number of cells expressing renin. Regulation of renin secretion by perfusion pressure, catecholamines, and angiotensin II from isolated kidneys of mice with renin cell-specific deletion of Cx45 was normal. Analyzing Cx45 promoter activity in cells of the preglomerular arteriolar tree by using mice driving the reporter gene LacZ under the control of the Cx45 promoter revealed strong staining in smooth muscle cells of the media, whereas renin-expressing cells were almost devoid of LacZ staining. Conversely, renin-producing cells, but not vascular smooth muscle cells expressed the gap junction protein Cx40. These findings suggest that Cx45 plays no major functional role in renin-producing cells, probably because the expression of Cx45 is downregulated in these cells. Since renin-producing cells in the adult kidney can reversibly transform into vascular smooth muscle cells and vice versa, our findings on connexin expression indicate that these phenotype switches are paralleled by characteristic reciprocal changes in the transcriptional activity of Cx40 and Cx45 genes.

scholarly journals Distribution of Gap Junction Protein Connexin 37 in Smooth Muscle Cells of the Rat Trachea and Pulmonary Artery.

1999 ◽  
Vol 62 (1) ◽  
pp. 27-37 ◽  
Author(s):  
Kyoko NAKAMURA ◽  
Tetsuichiro INAI ◽  
Keiichiro NAKAMURA ◽  
Yosaburo SHIBATA
Keyword(s):  
Smooth Muscle ◽  
Pulmonary Artery ◽  
Smooth Muscle Cells ◽  
Gap Junction ◽  
Muscle Cells ◽  
Junction Protein ◽  
Gap Junction Protein ◽  
Connexin 37 ◽  
Rat Trachea

F017 Differential effects of growth factors on gap junction expression in cultured human aortic smooth muscle cells

1998 ◽  
Vol 136 ◽  
pp. S51
Author(s):  
Hung-I Yen ◽  
Chryso Kanthou ◽  
Emmanuel Dupont ◽  
Florea Lupu ◽  
Nicholas J. Severs
Keyword(s):  
Smooth Muscle ◽  
Growth Factors ◽  
Smooth Muscle Cells ◽  
Gap Junction ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Differential Effects ◽  
Aortic Smooth Muscle

Sorghum Fermented by Aspergillus oryzae NK Enhances Inhibition of Vascular Inflammation in TNF-α-stimulated Human Aortic Smooth Muscle Cells

2018 ◽  
Vol 88 (5-6) ◽  
pp. 309-318
Author(s):  
Hae Seong Song ◽  
Jung-Eun Kwon ◽  
Hyun Jin Baek ◽  
Chang Won Kim ◽  
Hyelin Jeon ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Inflammatory Response ◽  
Smooth Muscle Cells ◽  
Adhesion Molecule ◽  
Vascular Inflammation ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Tnf Α ◽  
Cox 2

Abstract. Sorghum bicolor L. Moench is widely grown all over the world for food and feed. The effects of sorghum extracts on general inflammation have been previously studied, but its anti-vascular inflammatory effects are unknown. Therefore, this study investigated the anti-vascular inflammation effects of sorghum extract (SBE) and fermented extract of sorghum (fSBE) on human aortic smooth muscle cells (HASMCs). After the cytotoxicity test of the sorghum extract, a series of experiments were conducted. The inhibition effects of SBE and fSBE on the inflammatory response and adhesion molecule expression were measured using treatment with tumor necrosis factor-α (TNF-α), a crucial promoter for the development of atherosclerotic lesions, on HASMCs. After TNF-α (10 ng/mL) treatment for 2 h, then SBE and fSBE (100 and 200 μg/mL) were applied for 12h. Western blotting analysis showed that the expression of vascular cell adhesion molecule-1 (VCAM-1) (2.4-fold) and cyclooxygenase-2 (COX-2) (6.7-fold) decreased, and heme oxygenase-1 (HO-1) (3.5-fold) increased compared to the TNF-α control when treated with 200 μg/mL fSBE (P<0.05). In addition, the fSBE significantly increased the expression of HO-1 and significantly decreased the expression of VCAM-1 and COX-2 compared to the TNF-α control in mRNA level (P<0.05). These reasons of results might be due to the increased concentrations of procyanidin B1 (about 6-fold) and C1 (about 30-fold) produced through fermentation with Aspergillus oryzae NK for 48 h, at 37 °C. Overall, the results demonstrated that fSBE enhanced the inhibition of the inflammatory response and adherent molecule expression in HASMCs.

Calcifying Subpopulation of Bovine Aortic Smooth Muscle Cells Is Responsive to 17β-Estradiol

Circulation ◽  
1997 ◽  
Vol 95 (7) ◽  
pp. 1954-1960 ◽  
Author(s):  
Mihaela Balica ◽  
Kristina Boström ◽  
Victoria Shin ◽  
Kirsten Tillisch ◽  
Linda L. Demer
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
17Β Estradiol
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