A Study of Water in Glass by an Autoradiographic Method that Utilizes Tritiated Water

1983 ◽  
pp. 571-589 ◽  
Author(s):  
S. H. Knickerbocker ◽  
S. B. Joshi ◽  
S. D. Brown
Keyword(s):  
Tritiated Water ◽  
Autoradiographic Method

UPTAKE OF OXYTOCIN IN TISSUES AFTER INTRAVENOUS ADMINISTRATION OF TRITIATED OXYTOCIN IN RATS

1969 ◽  
Vol 61 (3) ◽  
pp. 432-440 ◽  
Author(s):  
Ingvar Sjöholm ◽  
Gunnar Rydén
Keyword(s):  
Skeletal Muscle ◽  
Distribution Pattern ◽  
Intravenous Injection ◽  
Intravenous Administration ◽  
Time Course ◽  
Tritiated Water ◽  
Preferential Distribution ◽  
Time Period ◽  
Initial Uptake

ABSTRACT The distribution of oxytocin in the kidneys, liver, uterus and skeletal muscle of the rat was followed during 10 min after intravenous injection of tritium labelled oxytocin. Oxytocin was found to be taken up and degraded mainly in the kidneys and the liver. After 150 seconds no intact oxytocin could be detected in these organs. The time course of the distribution of the radioactivity in the liver and the skeletal muscle showed no noteworthy characteristics, whereas a different course was found in the kidneys and in the uterus. In the kidneys, the radioactivity increased continuously from 60 to 200 seconds after the injection, indicating an accumulation of oxytocin or its metabolites in the kidneys. In the uterus a high initial uptake was observed, followed by a decrease of the radioactivity from 60 to 100 seconds after the injection. This distribution pattern was specific to oxytocin, since the uptake of tritiated tyrosine and tritiated water was almost constant during the same time period. These findings may indicate a preferential distribution of oxytocin to the uterus.

Variation in the wheat intake of individual sheep measured by use of labelled grain: behavioural influences

1981 ◽  
Vol 21 (111) ◽  
pp. 395 ◽  
Author(s):  
E Juwarini ◽  
B Howard ◽  
BD Siebert ◽  
JJ Lynch ◽  
RL Elwin
Keyword(s):  
Dominance Hierarchy ◽  
Tritiated Water ◽  
Supplementary Feeding ◽  
Previous Experience ◽  
Feed Consumption ◽  
Wheat Grain ◽  
Accurate Calculation ◽  
Early Introduction

A preliminary experiment with sheep in pens demonstrated that wheat grain could be labelled with tritiated water so that when fed it could provide data that would allow accurate calculation of individual feed consumption. This techinque was used with two groups of sheep fed supplementary wheat grain in paddocks. Half of the animals had previous experience of grain feeding some eight months earlier and the others had not eaten grain. Individual diversity of intake could be estimated usefully by tritium labelling of wheat, which was fed to the sheep in a group. The experiment showed that there was a threefold difference in the amount of wheat eaten between the lowest and highest intakes. Further, animals with previous experience of grain feeding consumed the entire ration initially, but those without previous experience did not consume all of the ration until two weeks after wheat feeding began. Over the period of measurement the experienced sheep consumed about 13% more wheat than the non-experienced group. There were insufficient aggressive acts to establish a dominance hierarchy in either group, although the experienced sheep were more aggressive than the others. Aggressiveness by one sheep towards other sheep did not result in higher wheat intakes by the former compared with other sheep in the group. The results are discussed in terms of the variability in acceptance of such supplements by animals, and of the value, later in life, of early introduction of supplementary feeding.

A TRITIATED WATER EXPERIMENT ON A MALE ATLANTIC WALRUS (ODOBENUS ROSMARUS ROSMARUS)

1992 ◽  
Vol 8 (4) ◽  
pp. 418-420 ◽  
Author(s):  
Christian Lydersen ◽  
David Griffiths ◽  
Ian Gjertz ◽  
Oystein Wiig
Keyword(s):  
Tritiated Water ◽  
Odobenus Rosmarus ◽  
Odobenus Rosmarus Rosmarus ◽  
Atlantic Walrus

Differential expression profiles of microRNA in mice internally exposed to tritiated water

2011 ◽  
Vol 93 (4) ◽  
pp. 738-751 ◽  
Author(s):  
Feng-Mei Cui ◽  
Xiu-Jin Sun ◽  
Xiang Ding ◽  
Qiu Chen ◽  
Jian-Ping Cao ◽  
...  
Keyword(s):  
Differential Expression ◽  
Expression Profiles ◽  
Tritiated Water

Aspects of the Water, Electrolyte and Carbohydrate Physiology of the Silvereye, Zosterops Lateralis (Aves).

1983 ◽  
Vol 31 (5) ◽  
pp. 695 ◽  
Author(s):  
IJ Rooke ◽  
SD Bradshaw ◽  
RA Langworthy
Keyword(s):  
Natural Habitat ◽  
Tritiated Water ◽  
Free Water ◽  
Laboratory Studies ◽  
Turnover Rates ◽  
Natural Habitats ◽  
Water Influx ◽  
Zosterops Lateralis ◽  
Total Body ◽  
Free Ranging

Total body water content (TBW) and TBW turnover were measured by means of tritiated water (HTO) in free-ranging populations of silvereyes, Zosterops lateralis, near Margaret River, W.A. Birds were studied in their natural habitats during spring and summer, and compared with a vineyard population in summer. In the natural habitat TBW content was found to be 77.6% in spring, which was not significantly different from that measured in summer (78.3%). Birds in vineyards in summer, however, were dehydrated, with a TBW content of 69.4%. Calculated rates of water influx for spring, summer and summer vineyards birds were 1.44,2.20 and 0.65 ml g.day-' respectively. These water turnover rates are much higher than those of any other bird yet studied. Dehydration was marked in the vineyard birds, with a significantly lower TBW content and an average net water loss of 0.63 ml day-'. Laboratory studies showed that silvereyes have a low tolerance to sodium loading. Their tolerance is, however, quite adequate for them to drink the most concentrated free water available to them in the field. Ingestion of concentrated sugar solutions of up to 25% did not provoke an osmotic diuresis and thus cannot account for the dehydration and negative water balance of vineyard birds.

Effect of Tritiated Water on Female Germ Cells: Mouse Oocyte Killing and RBE

1989 ◽  
Vol 56 (3) ◽  
pp. 293-299 ◽  
Author(s):  
Y. Satow ◽  
H. Hori ◽  
J.-Y. Lee ◽  
M. Ohtaki ◽  
S. Sawada ◽  
...  
Keyword(s):  
Germ Cells ◽  
Tritiated Water ◽  
Mouse Oocyte

Simple validation for the hepatic venous cannula implanted chronically in conscious rats

1991 ◽  
Vol 71 (1) ◽  
pp. 359-364 ◽  
Author(s):  
M. T. Huang
Keyword(s):  
Inferior Vena Cava ◽  
Inferior Vena ◽  
Superior Vena ◽  
Tritiated Water ◽  
Vena Cava ◽  
Systemic Circulation ◽  
Tracer Injection ◽  
Conscious Rats ◽  
Tygon Tubing ◽  
Venous Cannula

A method for the detection of vena caval contamination in blood taken from hepatic venous cannulas in conscious rats was described. The procedures included 1) bolus injection of tritiated water (50 microCi) through a cannula into the abdominal inferior vena cava and 2) continuous blood sampling (less than 0.2 ml) from the hepatic venous cannula for 2 min into a 180-cm piece of Tygon tubing, starting concurrently with tracer injection. The washout of tritium was determined from samples in 15-cm sections of Tygon tubing. Because circulation from the inferior vena cava to the hepatic vein is interceded by the systemic circulation, the washout of tritium from a valid hepatic venous cannula should resemble the pattern determined elsewhere in the systemic circulation. In the current study, the reference systemic washout was determined in the superior vena cava of a group of rats similarly injected with tritiated water in the inferior vena cava. The maximum of tritium washout derived from a valid hepatic venous cannula should fall in the range encompassed by one standard deviation of the mean of the maximum of the reference (1,400 to 1,930 cpm/sample). The maximum of the washout pattern derived from the invalid cannula, which lay adjacent to the site of injection, was expected to exceed this range. On the basis of these criteria, hepatic blood flow (HBF) was determined by sulfbromophthalein (BSP) extraction in groups of rats with valid and invalid cannulas. HBF in rats with valid hepatic venous cannulas was 2.58 +/- 0.15 in the conscious state and 2.76 +/- 0.26 ml.min-1.g wet wt-1 in the ketamine-anesthetized state.(ABSTRACT TRUNCATED AT 250 WORDS)

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