The Biosynthesis of Deoxyguanosine Triphosphate in Herpes Simplex Type-1 Infected Vero Cells Treated with Acyclovir and Hydroxyurea

1986 ◽  
pp. 151-156 ◽  
Author(s):  
Catherine U. Lambe ◽  
Donald J. Nelson ◽  
Phillip A. Furman
Keyword(s):  
Herpes Simplex ◽  
Vero Cells ◽  
Herpes Simplex Type ◽  
Deoxyguanosine Triphosphate

Cell sonicates used in the analysis of how measles and herpes simplex type 1 virus infections influence Vero cell mitochondrial calcium uptake

1985 ◽  
Vol 63 (11) ◽  
pp. 1194-1197 ◽  
Author(s):  
Karen Lund ◽  
Barry Ziola
Keyword(s):  
Herpes Simplex ◽  
Vero Cell ◽  
Measles Virus ◽  
Vero Cells ◽  
Lytic Cycle ◽  
Herpes Simplex Type ◽  
Uptake System ◽  
Virus Infections ◽  
Mitochondrial Calcium Uptake

Brief sonication was used to rapidly prepare an in situ mitochondrial Ca2+ uptake system from Vero cells. This method yielded intact, functional mitochondria capable of accumulating and retaining approximately 50% of Ca2+ available in the reaction mix. Mitochondria in cells infected with herpes simplex type 1 virus exhibited a gradual decline in the initial Ca2+ uptake rate, dropping to 65% of the control rate at the end of the 12-h lytic cycle. Influence of a lytic measles virus infection on the initial Ca2+ upake rate was different. The rate initially dropped to 75% of the control rate by 9 h postinfection, then recovered to slightly above the control rate at 21 h postinfection, and finally declined to 75% of the control rate at 30 h postinfection. Neither viral infection altered the total amount of Ca2+ accumulated by Vero cell mitochondria.

The effects of photodynamic therapy on Vero cells and replication of herpes simplex type 1: an in vitro analysis

2021 ◽  
Author(s):  
Rosângela Maria Callou Pinheiro ◽  
Maria Tereza Villela Romanos ◽  
Antonio Canabarro ◽  
Ana Cecília Aranha ◽  
Maíra Prado ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Herpes Simplex ◽  
Vero Cells ◽  
Herpes Simplex Type ◽  
Vitro Analysis ◽  
In Vitro Analysis

Brief Report: Inactivation of Herpesvirus on CPR Manikins Utilizing a Currently Recommended Disinfecting Procedure

1985 ◽  
Vol 6 (11) ◽  
pp. 456-458 ◽  
Author(s):  
Robert Z. Cavagnolo
Keyword(s):  
Herpes Simplex ◽  
Infectious Virus ◽  
Herpes Simplex Type

AbstractThe adequacy of a currently recommended protocol for disinfecting CPR manikins was investigated. Known quantities of Herpes simplex type 1 virus were applied to various sites on both adult and infant manikin heads, exposed to disinfectant under simulated classroom conditions, and then assayed for infectious virus. Results indicate that the disinfecting protocol and disinfectant are adequate for inactivating herpesvirus on CPR manikins, but that care must be exercised to ensure thorough cleaning.

Host strain-dependent difference in susceptibility in a rat model of herpes simplex type 1 encephalitis

2008 ◽  
Vol 14 (2) ◽  
pp. 102-118 ◽  
Author(s):  
Biborka Bereczky-Veress ◽  
Olle Lidman ◽  
Farideh Sabri ◽  
Ivan Bednar ◽  
Fredrik Granath ◽  
...  
Keyword(s):  
Herpes Simplex ◽  
Rat Model ◽  
Host Strain ◽  
Herpes Simplex Type ◽  
Strain Dependent

scholarly journals The Polysulfonated Compound Suramin Blocks Adsorption and Lateral Difusion of Herpes Simplex Virus Type-1 in Vero Cells

Virology ◽  
1999 ◽  
Vol 258 (1) ◽  
pp. 141-151 ◽  
Author(s):  
José S. Aguilar ◽  
Marcia Rice ◽  
Edward K. Wagner
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Vero Cells ◽  
Simplex Virus

scholarly journals The Conserved Carboxyl-Terminal Half of Herpes Simplex Virus Type 1 Regulatory Protein ICP27 Is Dispensable for Viral Growth in the Presence of Compensatory Mutations

2000 ◽  
Vol 74 (16) ◽  
pp. 7362-7374 ◽  
Author(s):  
Scott M. Bunnell ◽  
Stephen A. Rice
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Herpes Simplex Virus Type ◽  
Vero Cells ◽  
Terminal Portion ◽  
Viral Dna ◽  
Infected Cells ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACT ICP27 is an essential herpes simplex virus type 1 (HSV-1) immediate-early protein that regulates viral gene expression by poorly characterized mechanisms. Previous data suggest that its carboxyl (C)-terminal portion is absolutely required for productive viral infection. In this study, we isolated M16R, a second-site revertant of a viral ICP27 C-terminal mutant. M16R harbors an intragenic reversion, as demonstrated by the fact that its cloned ICP27 allele can complement the growth of an HSV-1 ICP27 deletion mutant. DNA sequencing demonstrated that the intragenic reversion is a frameshift alteration in a homopolymeric run of C residues at codons 215 to 217. This results in the predicted expression of a truncated, 289-residue molecule bearing 72 novel C-terminal residues derived from the +1 reading frame. Consistent with this, M16R expresses an ICP27-related molecule of the predicted size in the nuclei of infected cells. Transfection-based viral complementation assays confirmed that the truncated, frameshifted protein can partially substitute for ICP27 in the context of viral infection. Surprisingly, its novel C-terminal residues are required for this activity. To see if the frameshift mutation is all that is required for M16R's viability, we re-engineered the M16R ICP27 allele and inserted it into a new viral background, creating the HSV-1 mutant M16exC. An additional mutant, exCd305, was constructed which possesses the frameshift in the context of an ICP27 gene with the C terminus deleted. We found that both M16exC and exCd305 are nonviable in Vero cells, suggesting that one or more extragenic mutations are also required for the viability of M16R. Consistent with this interpretation, we isolated two viable derivatives ofexCd305 which grow productively in Vero cells despite being incapable of encoding the C-terminal portion of ICP27. Studies of viral DNA synthesis in mutant-infected cells indicated that the truncated, frameshifted ICP27 protein can enhance viral DNA replication. In summary, our results demonstrate that the C-terminal portion of ICP27, conserved widely in herpesviruses and previously believed to be absolutely essential, is dispensable for HSV-1 lytic replication in the presence of compensatory genomic mutations.

scholarly journals Review of Viral Encephalitis Cases Seen at a Tertiary Care Center in Turkey: Focus on Herpes Simplex Type 1

2017 ◽  
Vol 54 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Beyza CIFTCI KAVAKLIOGLU ◽  
Eda COBAN ◽  
Aysu SEN ◽  
Elif SOYLEMEZOGLU ◽  
Mehmet Ali ALDAN ◽  
...  
Keyword(s):  
Herpes Simplex ◽  
Viral Encephalitis ◽  
Care Center ◽  
Tertiary Care ◽  
Herpes Simplex Type ◽  
Tertiary Care Center
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