Brief Report: Inactivation of Herpesvirus on CPR Manikins Utilizing a Currently Recommended Disinfecting Procedure

Robert Z. Cavagnolo

doi:10.1017/s019594170006481x

Brief Report: Inactivation of Herpesvirus on CPR Manikins Utilizing a Currently Recommended Disinfecting Procedure

Infection Control ◽

10.1017/s019594170006481x ◽

1985 ◽

Vol 6 (11) ◽

pp. 456-458 ◽

Cited By ~ 4

Author(s):

Robert Z. Cavagnolo

Keyword(s):

Herpes Simplex ◽

Infectious Virus ◽

Herpes Simplex Type

AbstractThe adequacy of a currently recommended protocol for disinfecting CPR manikins was investigated. Known quantities of Herpes simplex type 1 virus were applied to various sites on both adult and infant manikin heads, exposed to disinfectant under simulated classroom conditions, and then assayed for infectious virus. Results indicate that the disinfecting protocol and disinfectant are adequate for inactivating herpesvirus on CPR manikins, but that care must be exercised to ensure thorough cleaning.

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Host strain-dependent difference in susceptibility in a rat model of herpes simplex type 1 encephalitis

Journal of NeuroVirology ◽

10.1080/13550280701883832 ◽

2008 ◽

Vol 14 (2) ◽

pp. 102-118 ◽

Cited By ~ 11

Author(s):

Biborka Bereczky-Veress ◽

Olle Lidman ◽

Farideh Sabri ◽

Ivan Bednar ◽

Fredrik Granath ◽

...

Keyword(s):

Herpes Simplex ◽

Rat Model ◽

Host Strain ◽

Herpes Simplex Type ◽

Strain Dependent

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Genetic Analysis of the Herpes Simplex Virus Type 1 UL20 Protein Domains Involved in Cytoplasmic Virion Envelopment and Virus-Induced Cell Fusion

Journal of Virology ◽

10.1128/jvi.78.14.7329-7343.2004 ◽

2004 ◽

Vol 78 (14) ◽

pp. 7329-7343 ◽

Cited By ~ 37

Author(s):

Jeffrey M. Melancon ◽

Timothy P. Foster ◽

Konstantin G. Kousoulas

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Cell Fusion ◽

Infectious Virus ◽

Virus Production ◽

Virion Morphogenesis ◽

Production Group ◽

Simplex Virus ◽

Mutant Genes

ABSTRACT The herpes simplex virus type 1 UL20 protein (UL20p) is an important determinant for cytoplasmic virion morphogenesis and virus-induced cell fusion. To delineate the functional domains of the UL20 protein, we generated a panel of single and multiple (cluster) alanine substitutions as well as UL20p carboxyl-terminal truncations. The UL20 mutant genes could be broadly categorized into four main groups: Group I UL20 mutant genes complemented for both virus production and virus-induced cell fusion; Group II UL20 mutant genes did not complement for either virus-induced cell fusion or infectious virus production; Group III UL20 mutant genes complemented for virus-induced cell fusion to variable extents but exhibited substantially decreased ability to complement UL20-null infectious virus production; Group IV mutant genes complemented for infectious virus production but had variable effects on virus-induced cell fusion; this group included two mutants that efficiently complemented for gBsyn3, but not for gKsyn1, virus-induced cell fusion. In addition, certain recombinant viruses with mutations in either the amino or carboxyl termini of UL20p produced partially syncytial plaques on Vero cells in the absence of any other virally encoded syncytial mutations. These studies indicated that the amino and carboxyl termini of UL20p contained domains that functioned both in infectious virus production and virus-induced cell fusion. Moreover, the data suggested that the UL20p's role in virus-induced cell fusion can be functionally separated from its role in cytoplasmic virion morphogenesis and that certain UL20p domains that function in gB-syn3 virus-induced cell fusion are distinct from those functioning in gKsyn1 virus-induced cell fusion.

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