Autoradiographic Analysis of Gonadotropin Binding to Rat Ovarian Tissue Sections

1973 ◽  
pp. 365-378 ◽  
Author(s):  
A. Rees Midgley
Keyword(s):  
Ovarian Tissue ◽  
Tissue Sections ◽  
Autoradiographic Analysis

Membrane Transport Properties of Equine and Macaque Ovarian Tissues Frozen in Mixtures of Dimethylsulfoxide and Ethylene Glycol

2007 ◽  
Vol 129 (5) ◽  
pp. 688-694 ◽  
Author(s):  
A. Kardak ◽  
S. P. Leibo ◽  
R. Devireddy
Keyword(s):  
Ethylene Glycol ◽  
Water Transport ◽  
Ovarian Tissue ◽  
Membrane Properties ◽  
Tissue Samples ◽  
Cryoprotective Agents ◽  
Tissue Sections ◽  
Ovarian Cells ◽  
Subzero Temperatures ◽  
Best Fit

The rate at which equine and macaque ovarian tissue sections are first cooled from +25°Cto+4°C has a significant effect on the measured water transport when the tissues are subsequently frozen in 0.85M solutions of glycerol, dimethylsulfoxide (DMSO), or ethylene glycol (EG). To determine whether the response of ovarian tissues is altered if they are suspended in mixtures of cryoprotective agents (CPAs), rather than in solutions of a single CPA, we have now measured the subzero water transport from ovarian tissues that were suspended in mixtures of DMSO and EG. Sections of freshly collected equine and macaque ovaries were suspended either in a mixture of 0.9M EG plus 0.7M DMSO (equivalent to a mixture of ∼5%v∕v of EG and DMSO) or in a 1.6M solution of only DMSO or only EG. The tissue sections were cooled from +25°Cto+4°C and then frozen to subzero temperatures at 5°C∕min. As the tissues were being frozen, a shape-independent differential scanning calorimeter technique was used to measure water loss from the tissues and, consequently, the best fit membrane permeability parameters (Lpg and ELp) of ovarian tissues during freezing. In the mixture of DMSO+EG, the respective values of Lpg and ELp for equine tissue first cooled at 40°C∕min between +25°C and +4°C before being frozen were 0.15μm∕minatm and 7.6kcal∕mole. The corresponding Lpg and ELp values for equine tissue suspended in 1.6M DMSO were 0.12μm∕minatm and 27.2kcal∕mole; in 1.6M EG, the values were 0.06μm∕minatm and 21.9kcal∕mole, respectively. For macaque ovarian tissues suspended in the mixture of DMSO+EG, the respective values of Lpg and ELp were 0.26μm∕minatm and 26.2kcal∕mole. Similarly, the corresponding LLg and ELp values for macaque tissue suspended in 1.6M DMSO were 0.22μm∕minatm and 31.4kcal∕mole; in 1.6M EG, the values were 0.20μm∕minatm and 27.9kcal∕mole. The parameters for both equine and macaque tissue samples suspended in the DMSO+EG mixture and first cooled at 0.5°C∕min between +25°C and +4°C were very similar to the corresponding values for samples cooled at 40°C∕min. In contrast, the membrane parameters of equine and macaque samples first cooled at 0.5°C∕min in single-component solutions were significantly different from the corresponding values for samples cooled at 40°C∕min. These results show that the membrane properties of ovarian cells from two species are different, and that the membrane properties are significantly affected both by the solution in which the tissue is suspended and by the rate at which the tissue is cooled from +25°Cto+4°C before being frozen. These observations suggest that these variables ought to be considered in the derivation of methods to cryopreserve ovarian tissues.

Histologic evaluation of parovarian nodules in the cat

2019 ◽  
Vol 22 (6) ◽  
pp. 571-574 ◽  
Author(s):  
Meghann L Haase-Berglund ◽  
Ching Yang ◽  
Christopher Premanandan
Keyword(s):  
Adrenal Gland ◽  
Ovarian Tissue ◽  
In Vivo Studies ◽  
Tissue Sections ◽  
Surgical Sterilization ◽  
Histologic Evaluation ◽  
Ectopic Adrenal Tissue ◽  
Larger Sample

Objectives A notable, although relatively uncommon, finding during feline ovariohysterectomy is the presence of parovarian nodules, located proximal to the ovary, near or within the ovarian vascular bundle. They are usually 2–3 mm in diameter, white-to-tan in color and glandular in appearance. The objective of this study was to either decisively reinforce the existing findings reported in the literature, which suggest that these parovarian nodules are incidental adrenocortical nodules that are clinically and surgically insignificant, or capture any samples that were not ectopic adrenal tissue in order to characterize them histologically. Methods Ninety-one formalin-preserved tissue sections containing parovarian nodules were collected during routine feline elective ovariohysterectomy and evaluated histologically. Results Definitive histologic information was obtained from 73/91 (80.2%) submitted samples. Fifty-two of 73 (71.2%) samples were determined to contain accessory adrenal gland cortex. Twenty of 73 (27.4%) samples were determined to contain residual mesonephric structures. While accessory adrenocortical nodules were found bilaterally in cats of all ages, mesonephric structures were predominantly right-sided, and only found in one cat over the age of 1 year. Ectopic or accessory ovarian tissue was not found in any of the samples. Conclusions and relevance This study adds to the existing body of data which suggest these nodules are incidental structures that do not need to be removed during surgical sterilization. However, further in vivo studies with larger sample sizes and years of follow-up would be required to more definitively prove this theory.

scholarly journals A new method for labeling and autoradiographic localization of androgen receptors.

1987 ◽  
Vol 35 (7) ◽  
pp. 755-762 ◽  
Author(s):  
C A Peters ◽  
E R Barrack
Keyword(s):  
Androgen Receptor ◽  
Androgen Receptors ◽  
Ventral Prostate ◽  
Tissue Sections ◽  
Receptor Localization ◽  
Rat Ventral Prostate ◽  
Dry Emulsion ◽  
Autoradiographic Analysis ◽  
Autoradiographic Technique ◽  
Silver Grains

We have used a novel receptor labeling and autoradiographic technique to localize androgen receptors in the intact rat ventral prostate at the morphological level. Frozen slide-mounted prostate tissue sections (10 micron thick) were incubated with increasing concentrations of [3H]-R1881 in the absence and presence of excess unlabeled R1881. Tissue sections labeled in this way were subjected to concurrent biochemical and autoradiographic analysis. After incubation and washing to remove free [3H]-steroid, some of the sections were wiped from the slides for scintillation counting in order to characterize and quantitate [3H]-R1881 binding. Androgen receptors could indeed be labeled in slide-mounted tissue sections, and specific [3H]-R1881 binding to these receptors was high-affinity (Kd = 1 nM), saturable, and androgen-specific. All cellular androgen receptors appear to be retained, because receptor content in sections was comparable to the sum of receptors in subcellular fractions of homogenized tissue. Replicate labeled slide-mounted tissue sections were dried rapidly, apposed to dry emulsion-coated coverslips, and exposed in the dark for autoradiography. Silver grains were counted over nuclei or cytoplasm of epithelium or stroma to evaluate specific androgen receptor location. Autoradiographic analysis demonstrated androgen receptor localization almost exclusively in the epithelial nuclei, with little or none in the stroma. We discuss here the unique features and advantages of labeling androgen receptors in slide-mounted frozen tissue sections for autoradiographic localization.

scholarly journals Polarity switching mass spectrometry imaging of healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 595-605 ◽  
Author(s):  
Milad Nazari ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Ovarian Tissue ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Cancer Tissue ◽  
Ovarian Cancer Tissue ◽  
Tissue Sections ◽  
Polarity Switching ◽  
Matrix Assisted Laser

IR-MALDESI polarity switching mass spectrometry imaging reveals differences in lipid distribution in hen ovarian cancer tissue.

scholarly journals Quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

The Analyst ◽  
2018 ◽  
Vol 143 (3) ◽  
pp. 654-661 ◽  
Author(s):  
Milad Nazari ◽  
Mark T. Bokhart ◽  
Philip L. Loziuk ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Ovarian Tissue ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Quantitative Mass Spectrometry ◽  
Tissue Sections ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

IR-MALDESI quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissues.

113. Freezing characteristics of macaque and equine ovarian tissue sections in mixtures of dimethyl sulfoxide and ethylene glycol

Cryobiology ◽  
2006 ◽  
Vol 53 (3) ◽  
pp. 415-416 ◽  
Author(s):  
Ajay Kardak ◽  
Stanley P. Leibo ◽  
Ramachandra V. Devireddy
Keyword(s):  
Ethylene Glycol ◽  
Dimethyl Sulfoxide ◽  
Ovarian Tissue ◽  
Tissue Sections

scholarly journals Changes in Transcriptomic Profiles in Different Reproductive Periods in Yaks

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1229
Author(s):  
Shaoke Guo ◽  
Mengli Cao ◽  
Xingdong Wang ◽  
Lin Xiong ◽  
Xiaoyun Wu ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Ovarian Tissue ◽  
Follicular Development ◽  
Ovarian Activity ◽  
Regulation Mechanism ◽  
Rna Seq ◽  
Genomic Expression ◽  
Tissue Sections ◽  
Comparison Groups ◽  
Essential Knowledge

Yak reproductive characteristics have received extensive attention, though the molecular regulation mechanism of its ovarian activity remains to be explored. Therefore, this study initially conducted a comparative analysis of yak ovarian activities in anestrus, estrus, and pregnancy regarding their morphology and histology, followed by implementing RNA sequencing (RNA-seq) technology to detect the overall gene expression and biological mechanism in different reproductive stages. H&E staining showed that there were more growing follicles and mature follicles in ovarian tissue sections during estrus than ovarian tissues during non-estrus. The RNA-seq analysis of yak ovary tissues in three periods showed that DEGs related to follicular development and hormone metabolism were screened in the three comparison groups, such as COL1A2, NR4A1, THBS2, PTGS2, SCARB1, STAR, and WNT2B. Bioinformatics analysis showed that these DEGs are involved in ion binding, cell development, metabolic processes, enriched in ECM–receptor interactions, steroid biosynthesis, together with aldosterone generation/discharge and Wnt/PI3K-Akt signaling pathways. In addition, we speculate alternate splice development events to have important role/s in regulating ovarian functional genomic expression profiles. These results provide essential knowledge aimed at scrutinizing pivotal biomarkers for yak ovarian activity, together with paving the way for enhancing researchers’ focus on improving yak reproductive performance.

42 THE EFFECT OF VARIOUS CRYOPROTECTIVE AGENTS AND SLOW COOLING RATE ON VIABILITY OF SHEEP OVARIAN TISSUE

2017 ◽  
Vol 29 (1) ◽  
pp. 128
Author(s):  
A. S. Seisenbayeva ◽  
Y. M. Toishibekov ◽  
U. I. Iglmanov ◽  
B. A. Valieva
Keyword(s):  
Ovarian Tissue ◽  
Passive Cooling ◽  
Slow Cooling ◽  
Fresh Tissue ◽  
Cryoprotective Agents ◽  
Tissue Sections ◽  
Round Nucleus ◽  
Hematoxylin And Eosin ◽  
Comparative Histology

Priority objects of protection in agrobiocenoses are grades of cultural plants and local breeds of the cultivated animals. The most general criteria for preservation of local breeds are viability, adaptability, a state of health, reproductive abilities, and unique genetic polymorphism at the molecular and morphological levels. Therefore, the aim of this study was to compare the effectiveness of different cryoprotectors on morphology of ovine ovarian tissue cryopreserved by a passive cooling method. Ovarian tissue from 10 indigenous Chuyi breed was transported to the laboratory within 30 min at 32 to 34°C, divided into smaller pieces (2.0 × 1.2 × 1.0 mm), and randomly distributed into 4 groups: (1) control (fresh tissue), (2) pieces after freezing/thawing with 1.5 M ethylene glycol (EG); (3) pieces after freezing/thawing with 1.5 M propanediol (PROH); (4) pieces after freezing/thawing with 1.5 M dimethyl sulfoxide (DMSO). The ovarian pieces were placed in a cryovial and equilibrated sequentially in freezing medium containing 0.25, 0.75, and 1.5 M cryoprotectors with 0.5 M sucrose (5 min each), precooled at 4°C, and stored in a −80°C freezer for 24 h. Then, the cryovials containing the ovarian pieces were placed in liquid nitrogen and stored (for 2 months) until thawing. The ovarian pieces were cultured in vitro for 7 days in TCM-HEPES+ 10% native ovine serum (NOS) (Seisenbayeva et al. 2015 Reprod. Fertil. Dev. 28, 193–194). After 7 days of culture, we evaluated the effects of passive cooling methods with different cryoprotectors on ovarian tissue morphology by light microscopy after hematoxylin and eosin staining of tissue sections. The number of viable and damaged follicles was counted. All morphologically normal primordial, primary, and secondary follicles had healthy and intact oocytes, each containing a round nucleus and clearly visible nucleolus surrounded by well-organised granulosa cells without a pyknotic nucleus. Integrity rate of tissue after treatment was evaluated by Student’s test. In groups 1, 2, 3, and 4, the mean densities of follicles per 1 mm3 was 17.0 ± 4.6a, 16.2 ± 7.2b, 13.0 ± 5.1c, and 11.9 ± 4.8d, respectively (ab, ac, ad P > 0.05). For these groups, respectively, 69.2 ± 8.2%a , 61.7 ± 8.6%b , 52.4 ± 8.8%c and 48.5 ± 8.8%d preantral follicles were morphologically normal (ab, ac, ad P > 0.05). We did not find significant differences between groups. The analysis of comparative histology shows that 1.5 M EG is more effective on viability of ovarian follicles than 1.5 M DMSO or 1.5 M PROH.

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