scholarly journals Polarity switching mass spectrometry imaging of healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 595-605 ◽  
Author(s):  
Milad Nazari ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Ovarian Tissue ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Cancer Tissue ◽  
Ovarian Cancer Tissue ◽  
Tissue Sections ◽  
Polarity Switching ◽  
Matrix Assisted Laser

IR-MALDESI polarity switching mass spectrometry imaging reveals differences in lipid distribution in hen ovarian cancer tissue.

scholarly journals Quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

The Analyst ◽  
2018 ◽  
Vol 143 (3) ◽  
pp. 654-661 ◽  
Author(s):  
Milad Nazari ◽  
Mark T. Bokhart ◽  
Philip L. Loziuk ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Ovarian Tissue ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Quantitative Mass Spectrometry ◽  
Tissue Sections ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

IR-MALDESI quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissues.

scholarly journals Mass spectrometry imaging (MSI) of fresh bones using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

2019 ◽  
Vol 11 (46) ◽  
pp. 5929-5938 ◽  
Author(s):  
Sitora Khodjaniyazova ◽  
Nicholas J. Hanne ◽  
Jacqueline H. Cole ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Direct Analysis ◽  
Chemical Treatments ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Direct analysis and IR-MALDESI mass spectrometry imaging of fresh mouse bones that underwent no chemical treatments other than flash-freezing.

scholarly journals Mass Spectrometry Imaging Reveals Heterogeneous Efavirenz Distribution within Putative HIV Reservoirs

2015 ◽  
Vol 59 (5) ◽  
pp. 2944-2948 ◽  
Author(s):  
Corbin G. Thompson ◽  
Mark T. Bokhart ◽  
Craig Sykes ◽  
Lourdes Adamson ◽  
Yuri Fedoriw ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Imaging Data ◽  
Hiv Replication ◽  
Viral Reservoirs ◽  
Antiretroviral Drug ◽  
The Brain ◽  
Matrix Assisted Laser

ABSTRACTPersistent HIV replication within active viral reservoirs may be caused by inadequate antiretroviral penetration. Here, we used mass spectrometry imaging with infrared matrix-assisted laser desorption–electrospray ionization to quantify the distribution of efavirenz within tissues from a macaque dosed orally to a steady state. Intratissue efavirenz distribution was heterogeneous, with the drug concentrating in the lamina propria of the colon, the primary follicles of lymph nodes, and the brain gray matter. These are the first imaging data of an antiretroviral drug in active viral reservoirs.

scholarly journals Enhancing Metabolomic Coverage in Positive Ionization Mode Using Dicationic Reagents by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization

Metabolites ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 810
Author(s):  
Ying Xi ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Rat Liver ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Ionization Mode ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Mass spectrometry imaging is a powerful tool to analyze a large number of metabolites with their spatial coordinates collected throughout the sample. However, the significant differences in ionization efficiency pose a big challenge to metabolomic mass spectrometry imaging. To solve the challenge and obtain a complete data profile, researchers typically perform experiments in both positive and negative ionization modes, which is time-consuming. In this work, we evaluated the use of the dicationic reagent, 1,5-pentanediyl-bis(1-butylpyrrolidinium) difluoride (abbreviated to [C5(bpyr)2]F2) to detect a broad range of metabolites in the positive ionization mode by infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging (IR-MALDESI MSI). [C5(bpyr)2]F2 at 10 µM was doped in 50% MeOH/H2O (v/v) electrospray solvent to form +1 charged adducted ions with anionic species (−1 charged) through post-electrospray ionization. This method was demonstrated with sectioned rat liver and hen ovary. A total of 73 deprotonated metabolites from rat liver tissue sections were successfully adducted with [C5(bpyr)2]2+ and putatively identified in the adducted positive ionization polarity, along with 164 positively charged metabolite ions commonly seen in positive ionization mode, which resulted in 44% increased molecular coverage. In addition, we were able to generate images of hen ovary sections showing their morphological features. Following-up tandem mass spectrometry (MS/MS) indicated that this dicationic reagent [C5(bpyr)2]2+ could form ionic bonds with the headgroup of glycerophospholipid ions. The addition of the dicationic reagent [C5(bpyr)2]2+ in the electrospray solvent provides a rapid and effective way to enhance the detection of metabolites in positive ionization mode.

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