ESEM Methodology for the Study of Ice Samples at Environmentally Relevant Subzero Temperatures: “Subzero ESEM”

Frozen aqueous solutions are an important subject of study in numerous scientific branches including the pharmaceutical and food industry, atmospheric chemistry, biology, and medicine. Here, we present an advanced environmental scanning electron microscope methodology for research of ice samples at environmentally relevant subzero temperatures, thus under conditions in which it is extremely challenging to maintain the thermodynamic equilibrium of the specimen. The methodology opens possibilities to observe intact ice samples at close to natural conditions. Based on the results of ANSYS software simulations of the surface temperature of a frozen sample, and knowledge of the partial pressure of water vapor in the gas mixture near the sample, we monitored static ice samples over several minutes. We also discuss possible artifacts that can arise from unwanted surface ice formation on, or ice sublimation from, the sample, as a consequence of shifting conditions away from thermodynamic equilibrium in the specimen chamber. To demonstrate the applicability of the methodology, we characterized how the true morphology of ice spheres containing salt changed upon aging and the morphology of ice spheres containing bovine serum albumin. After combining static observations with the dynamic process of ice sublimation from the sample, we can attain images with nanometer resolution.

Fracture Morphology Analysis of Frozen Red Sandstone under Impact

The deformation and failure characteristics of red sandstone under subzero temperature were studied by the split Hopkinson pressure bar (SHPB) dynamic impact test. The effects of different subzero temperatures on rock strength properties, fractal dimension, and dissipated energy were analyzed combined with microfracture morphology. The reasons for rock dynamic mechanical property deterioration under lower subzero temperatures were revealed. The research shows that low subzero temperature will cause “frostbite” of red sandstone. Under high strain rate loading, the rock will quickly lose its bearing capacity, and its dynamic mechanical strength will drop sharply. The dissipated energy W L of the frozen rock specimen is positively correlated with the fractal dimension D and closely related to the macroscopic failure characteristics. It could be concluded that greater dissipation energy leads to more serious damage of rock and accordingly results in a larger fractal dimension. Fracture morphology analysis shows that the lower subzero temperature generated remarkable cracks in the material interface of the red sandstone. The damage of the red sandstone could be explained by the fact that the crack tip had low plastic deformation ability under high strain rate loading and the composition of cement was vulnerable to the subzero temperature effect.

Method for determining the viability of eggs of strongylates of the gastrointestinal tract of horses at critically low temperatures in Yakutia

It is known that the climate of Yakutia is much harsh and hard to tolerate by any living organism. It is considered that the peculiarities of adaptation to the conditions of the Far North are determined by the influence of special natural factors, which is a universal phenomenon of influence on all living organisms. Parasitic nematodes are found in all zones of Yakutia and in almost all domestic animals, among which representatives of the Strongylata suborder dominate. As the authors point out, the parasitization of strongylates in the gastrointestinal tract of herd horses causes great damage, since they reduce not only the productivity of animals, but also cause it to be difficult to endure the winter. Even at subzero temperatures, strongylate eggs are able to maintain their viability. In this paper, the authors of the article were able to present material on determining the ability to maintain the viability of strongylate eggs at low temperatures in Yakutia. For the first time, the results on the survival of strongylate eggs at critically low temperatures of the Yakut winter were obtained and interesting results were obtained, which are described in this article.

Ultra-Stretchable and Self-Healing Anti-Freezing Strain Sensors Based on Hydrophobic Associated Polyacrylic Acid Hydrogels

Water-rich conductive hydrogels with excellent stretchability are promising in strain sensors due to their potential application in flexible electronics. However, the features of being water-rich also limit their working environment. Hydrogels must be frozen at subzero temperatures and dried out under ambient conditions, leading to a loss of mechanical and electric properties. Herein, we prepare HAGx hydrogels (a polyacrylic acid (HAPAA) hydrogel in a binary water–glycerol solution, where x is the mass ratio of water to glycerol), in which the water is replaced with water–glycerol mixed solutions. The as-prepared HAGx hydrogels show great anti-freezing properties at a range of −70 to 25 °C, as well as excellent moisture stability (the weight retention rate was as high as 93% after 14 days). With the increase of glycerol, HAGx hydrogels demonstrate a superior stretchable and self-healing ability, which could even be stretched to more than 6000% without breaking, and had a 100% self-healing efficiency. The HAGx hydrogels had good self-healing ability at subzero temperatures. In addition, HAGx hydrogels also had eye-catching adhesive properties and transparency, which is helpful when used as strain sensors.