Hormone Action in Human Breast Cancer Estrogen Regulation of Specific Proteins

1983 ◽  
pp. 1-25 ◽  
Author(s):  
David J. Adams ◽  
Dean P. Edwards ◽  
William L. McGuire
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Hormone Action ◽  
Human Breast ◽  
Estrogen Regulation ◽  
Specific Proteins

Estrogen Regulation of Human Breast Cancer Cell Line MCF-7 Tissue Plasminogen Activator*

Endocrinology ◽  
1989 ◽  
Vol 125 (1) ◽  
pp. 492-500 ◽  
Author(s):  
HERBERT W. DICKERMAN ◽  
HECTOR L. MARTINEZ ◽  
JAMES I. SEEGER ◽  
S. ANAND KUMAR
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Breast Cancer Cell Line ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Estrogen Regulation ◽  
Tissue Plasminogen ◽  
Mcf 7

scholarly journals Estrogen regulation and ion dependence of taurine uptake by MCF-7 human breast cancer cells

2007 ◽  
Vol 12 (3) ◽  
Author(s):  
David Shennan ◽  
Jean Thomson
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast ◽  
Estrogen Regulation ◽  
Absolute Dependence ◽  
17Β Estradiol ◽  
Taurine Uptake ◽  
Mcf 7

AbstractIt has been reported that estrogen receptor-positive MCF-7 cells express TauT, a Na+-dependent taurine transporter. However, there is a paucity of information relating to the characteristics of taurine transport in this human breast cancer cell line. Therefore, we have examined the characteristics and regulation of taurine uptake by MCF-7 cells. Taurine uptake by MCF-7 cells showed an absolute dependence upon extracellular Na+. Although taurine uptake was reduced in Cl- free medium a significant portion of taurine uptake persisted in the presence of NO3 -. Taurine uptake by MCF-7 cells was inhibited by extracellular β-alanine but not by L-alanine or L-leucine. 17β-estadiol increased taurine uptake by MCF-7 cells: the Vmax of influx was increased without affecting the Km. The effect of 17β-estradiol on taurine uptake by MCF-7 cells was dependent upon the presence of extracellular Na+. In contrast, 17β-estradiol had no significant effect on the kinetic parameters of taurine uptake by estrogen receptor-negative MDA-MB-231 cells. It appears that estrogen regulates taurine uptake by MCF-7 cells via TauT. In addition, Na+-dependent taurine uptake may not be strictly dependent upon extracellular Cl-.

scholarly journals Estrogen regulation of transferrin gene expression in MCF-7 human breast cancer cells

2002 ◽  
Vol 29 (3) ◽  
pp. 305-317 ◽  
Author(s):  
C Vyhlidal ◽  
X Li ◽  
S Safe
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Estrogen Regulation ◽  
Tf Gene ◽  
Mcf 7

Transferrin (Tf) is an iron transport protein expressed in MCF-7 human breast cancer cells. In nuclear run-on assays, 17beta-estradiol (E2) increased the rate of Tf gene expression approximately 3-fold within 1 h after treatment and reporter gene activity was also induced in MCF-7 cells transfected with a construct containing a -3600 to +39 Tf gene promoter insert. Deletion and mutation analysis identified an E2-responsive promoter region between -811 and -762, which was GC-rich (80%) and contained two nonconsensus estrogen response elements (EREs). E2-responsiveness of this region was associated with a GGACA(N)(3)TGGCC motif (-803 to -791) which bound human estrogen receptor alpha (hERalpha) in gel mobility shift assays. In Drosophila Schneider SL-2 cells, the -811 to -752 was E2-responsive after cotransfection with hERalpha expression plasmid plus E2, whereas Sp1 protein did not induce transactivation. These studies confirm that E2 induces Tf gene expression through a nonconsensus distal ERE.

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