Direct in Vitro Effects of 1,25 (OH) 2 Vitamin D3 on Phosphate Transport in Isolated Enterocytes from Normal or Vitamin D Deficient Rats

1984 ◽  
pp. 181-188 ◽  
Author(s):  
Gérard Karsenty ◽  
Bernard Lacour ◽  
André Ulmann ◽  
Evelyne Pierandrei ◽  
Tilman Drüeke
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Phosphate Transport ◽  
Isolated Enterocytes ◽  
In Vitro Effects

In vitro effects of aluminum on bone phosphatases: A possible interaction with bPTH and vitamin D3 metabolites

1982 ◽  
Vol 34 (1) ◽  
pp. 280-284 ◽  
Author(s):  
M. Lieberherr ◽  
B. Grosse ◽  
G. Cournot-Witmer ◽  
C. L. Thil ◽  
S. Balsan
Keyword(s):  
Vitamin D3 ◽  
Vitamin D3 Metabolites ◽  
In Vitro Effects ◽  
Bone Phosphatases

In Vitro Cellular Muscle Calcium Metabolism. Characterization of Effects of 1,25-Dihydroxy-Vitamin D3 and 25-Hydroxy-Vitamin D3

1985 ◽  
Vol 40 (1-2) ◽  
pp. 102-108 ◽  
Author(s):  
Ana R. de Boland ◽  
Ricardo Boland
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Plasma Membranes ◽  
Selective Inhibition ◽  
Ruthenium Red ◽  
Ca Uptake ◽  
25 Hydroxy Vitamin D ◽  
Muscle Calcium

Cultures of vitamin D-deficient chick soleus muscle and 12 day-old chick embryo myoblasts were used to characterize the effects of 1,25-dihydroxy-vitamin D3 and 25-hydroxy-vitamin D3 on muscle cell Ca metabolism. Physiological amounts of both sterols increased the rate and extent of 45Ca uptake by cultures. However. 1.25(OH)2D3 was significantly more effective than 25 OHD3. The greater potency of 1,25(OH)2D3 to increase Ca uptake could be shown after various treatment intervals of cultures and using a wide concentration range of both derivatives. Information about Ca pools affected by vitamin D3 metabolites was obtained through kinetic analysis of Ca efflux in cultured myoblasts. Cytoplasmic and mitochondria Ca pools were identified on the basis of their half-times of desaturation and by selective inhibition of plasma membrane and mitochondrial Ca transport with LaCl3 and Ruthenium Red, respectively. The data suggests that 1,25(OH)2D3 acts on muscle cellular Ca by increasing Ca efflux and influx through mitochondrial and plasma membranes whereas the predominant effect of 25 OHD3 is to increase Ca influx into mitochondria.

scholarly journals Development and Characterization of an Orodispersible Film for Vitamin D3 Supplementation

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5851
Author(s):  
Irma Elisa Cupone ◽  
Eleonora Dellera ◽  
Fabio Marra ◽  
Andrea Maria Giori
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Dosage Form ◽  
Daily Intake ◽  
Stability Study ◽  
Food Supplements ◽  
Oral Dosage ◽  
Residual Water ◽  
Tract Infections

Vitamin D plays a crucial and very well-known role in regulation of calcium homeostasis and bone metabolism and mineralization. However, a huge and more recent body of evidence supports the positive influence of vitamin D on the regulation of immune response, ranging from protection against respiratory tract infections to prevention and management of asthma. Nevertheless, vitamin D deficiency is a very common condition and there is an increasing need for suitable products for proper supplementation, allowing good compliance also in specific populations. Orally disintegrating tablets (ODT) were first developed to overcome the difficulty experienced by pediatric and geriatric patients of swallowing traditional oral dosage forms and, recently, orodispersible films (ODF) are gaining popularity as novel dosage form for assuming active pharmaceutical ingredients, vitamins, and ingredients for food supplements. This study describes a 2000 IU Vitamin D3 ODF for daily intake, consisting of hydrophilic polymers and suitable excipients, manufactured by film-casting process. Elongation-at-break (E%), Young’s modulus (Y), and tensile strength (TS) were investigated using a dynamometer. Chemical stability was evaluated assaying the vitamin D3 in the films stored at different environmental conditions. In addition, in vitro disintegration and dissolution studies were performed. Correlation existed between the mechanical properties of the film and the residual water, acting as plasticizer. The stability study showed that vitamin D3 assay was ≥90% also after 3 months at 40 °C. The film disintegrated in less than 1 min and the vitamin D3 released was ≥75% after 15 min. An ODF with suitable properties can be manufactured and used as innovative dosage form for vitamin D3 food supplements.

Renal effect of vitamin D metabolites: evidence for the essential role of the 25(OH) group

1983 ◽  
Vol 244 (6) ◽  
pp. F674-F678 ◽  
Author(s):  
M. M. Friedlaender ◽  
Z. Kornberg ◽  
H. Wald ◽  
M. M. Popovtzer
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Essential Role ◽  
Fractional Excretion ◽  
Vitamin D Metabolites ◽  
Group 2 ◽  
Fractional Excretion Of Phosphate ◽  
Group 1

The effects of 1 alpha (OH)vitamin D3 [1 alpha (OH)D3] and 24,25(OH)2vitamin D3 [24,25(OH)2D3] on the phosphaturic action of parathyroid hormone (PTH) were studied in two groups of parathyroidectomized (PTX) rats. In group 1, PTX PTH-infused rats received intravenous 1 alpha (OH)D3, and in group 2, PTX PTH-infused rats received intravenous 24,25(OH)2D3. PTX PTH-infused rats served as controls. The effects of both vitamin D metabolites on renal PTH-activated adenylate cyclase (AC) were studied in vitro. In group 1, PTH increased fractional excretion of phosphate (CP/CIn) from 0.045 +/- 0.012 (+/- SE) to 0.263 +/- 0.011 (P less than 0.005). 1 alpha (OH)D3 failed to influence this response. In group 2, PTH increased CP/CIn from 0.055 +/- 0.008 to 0.289 +/- 0.027 (P less than 0.005). 24,25(OH)2D3 reduced the PTH-induced rise in CP/CIn from 0.289 +/- 0.027 to 0.192 +/- 0.021 (P less than 0.01) and decreased the urinary excretion of adenosine 3',5'-cyclic monophosphate. In vitro, 24,25(OH)2D3 blunted the PTH-activated AC, whereas 1 alpha (OH)D3 had no effect. These results show that 24,25(OH)D3, similar to two other 25(OH) metabolites of vitamin D-25(OH)vitamin D3 and 1,25(OH)2vitamin D3-suppresses the phosphaturic action of PTH, whereas 1 alpha(OH)D3, which is devoid of a 25(OH) group, lacks this effect. This suggests that a 25(OH) group is a prerequisite for the antiphosphaturic effect of vitamin D, whereas the 1 alpha (OH) group is not essential for this action.

scholarly journals Vitamin D enhances responses to interferon-β in MS

2019 ◽  
Vol 6 (6) ◽  
pp. e622 ◽  
Author(s):  
Xuan Feng ◽  
Zhe Wang ◽  
Quentin Howlett-Prieto ◽  
Nathan Einhorn ◽  
Suad Causevic ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Immune Regulation ◽  
Mononuclear Cells ◽  
Serum Proteins ◽  
Multiplex Assay ◽  
Interferon Β ◽  
Western Blots ◽  
Th17 Cytokines

ObjectiveTo determine the effect of vitamin D3 on interferon-β (IFN-β) response and immune regulation in MS mononuclear cells (MNCs).MethodsMNCs from 126 subjects, including therapy-naive patients with different forms of MS, plus patients with MS receiving IFN-β or glatiramer treatment, plus healthy controls were incubated in vitro with IFN-β-1b ± vitamin D3 (calcitriol). Activation of the IFN-β–induced transcription factor, p-Y-STAT1, and antiviral myxovirus A (MxA) protein was measured with flow cytometry and Western blots; serum proteins were measured with a customized 31-protein multiplex assay.ResultsVitamin D enhanced in vitro IFN responses, as measured by induction of p-Y-STAT1 and MxA in MNCs, T cells, and monocytes. Vitamin D augmentation of IFN responses was seen in untreated and in IFN-β-1b–treated MS. The combination of vitamin D plus IFN-β reduced Th1 and Th17 cytokines, and increased Th2 responses, reversing the effect of IFN-β alone. Exacerbations and progression in untreated patients reduced the vitamin D enhancement of IFN responses. Vitamin D had less effect on IFN response in clinically stable glatiramer-treated than in IFN-β–treated patients.ConclusionVitamin D enhances IFN-β induction of multiple proteins and also reverses the Th1/Th2 bias in MS seen with IFN-β alone. The combination of vitamin D and IFN-β has potential benefit in ameliorating MS.

scholarly journals Calcium Absorptive Effects of Vitamin D and Its Major Metabolites1

1997 ◽  
Vol 82 (12) ◽  
pp. 4111-4116 ◽  
Author(s):  
Robert P. Heaney ◽  
M. Janet Barger-Lux ◽  
M. Susan Dowell ◽  
Tai C. Chen ◽  
Michael F. Holick
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Serum Vitamin ◽  
Standard Test ◽  
Molar Ratio ◽  
Response Curves ◽  
Serum Vitamin D ◽  
Adult Men ◽  
Calcium Load

The absorptive response to graded doses of vitamin D3, 25(OH)D, and 1,25(OH)2D was measured in healthy adult men after treatment periods of eight, four, and two weeks, respectively. While no relationship was found between baseline absorption and serum vitamin D metabolite levels, all three vitamin D compounds significantly elevated 45Ca absorption from a 300 mg calcium load given as part of a standard test meal. 1,25(OH)2D was active even at the lowest dose (0.5μ g/day), and the slope was such that doubling of absorption would occur at an oral dose of approximately 3 μg/day. 25(OH)D was also active in elevating absorption and did so without raising total 1,25(OH)2D levels. On the basis of the dose response curves for 1,25(OH)2D and 25(OH)D, the two compounds exhibited a molar ratio for physiological potency of approximately 100:1. The absorptive effect of vitamin D3 was seen only at the highest dose level (1250 μg, or 50,000 IU/day) and was apparently mediated by conversion to 25(OH)D. Analysis of the pooled 25(OH)D data from both the 25(OH)D- and vitamin D3-treated groups suggests that approximately one eighth of circulating vitamin D-like absorptive activity under untreated conditions in winter may reside in 25(OH)D. This is a substantially larger share than has been predicted from studies of in vitro receptor binding.

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