A Continuous Wave Spectroscopic (CWS) Study of Hemoprotein and Other Molecules in Mitochondrial Suspension, Cell Suspension and Tissue

Cell suspension cultures of Jatropha curcas were established and optimized in shake flasks. The stem segments of Jatropha curcas were taken as the explants for studying the techniques of callus induction and cell suspension cultures. The result shows that the optimal medium for callus induction is MS+2,4-D0.6mg/L+ BA1.0mg/L + Sucrose 30g/L, in which the callus is humid, loose and colorful. The fine suspension cell system have been established by inoculating the callus in the medium of MS+NAA0.2mg/L+2,4-D1.0mg/L+BA0.5mg/L for 13 days of cultivation, and the rotation speed should be lower than 120rpm in the culture of oscillation. QD-labeled chitosan-DNA complexes as nano transgenic system, using CdSe as bio-labels and chitosan-DNA(CS-DNA) as nano-scale genic carriers, were prepared and shown to have uniform particle sizes and superior fluorescence properties. Confocal laser scanning microscopy(CLSM) confirmed the target DNA from QD-labeled chitosan-DNA complexes was integrated into the plant cell and suggest the possibility of stable transformation in Jatropha curcas.

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Acaricidal activities of whole cell suspension, cell-free supernatant, and crude cell extract of Xenorhabdus stokiae against mushroom mite (Luciaphorus sp.)

Journal of Zhejiang University SCIENCE B ◽

10.1631/jzus.b1100155 ◽

2012 ◽

Vol 13 (4) ◽

pp. 261-266 ◽

Cited By ~ 11

Author(s):

Prapassorn Bussaman ◽

Chirayu Sa-Uth ◽

Paweena Rattanasena ◽

Angsumarn Chandrapatya

Keyword(s):

Cell Suspension ◽

Cell Extract ◽

Suspension Cell ◽

Crude Cell Extract ◽

Whole Cell ◽

Cell Free Supernatant

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Microstructures of Laser Processed Fe-Cr Surface Alloys

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100098150 ◽

1981 ◽

Vol 39 ◽

pp. 328-329

Author(s):

P. A. Molian ◽

K. H. Khan ◽

W. E. Wood

Keyword(s):

Cooling Rate ◽

Pure Iron ◽

Continuous Wave ◽

Laser Surface ◽

Material Surface ◽

Constitution Diagram ◽

Incident Power ◽

Surface Alloying ◽

Laser Surface Alloying ◽

Spot Diameter

In recent years, the effects of chromium on the transformation characteristics of pure iron and the structures produced thereby have been extensively studied as a function of cooling rate. In this paper, we present TEM observations made on specimens of Fe-10% Cr and Fe-20% Cr alloys produced through laser surface alloying process with an estimated cooling rate of 8.8 x 104°C/sec. These two chromium levels were selected in order to study their phase transformation characteristics which are dissimilar in the two cases as predicted by the constitution diagram. Pure iron (C<0.01%, Si<0.01%, Mn<0.01%, S=0.003%, P=0.008%) was electrodeposited with chromium to the thicknesses of 40 and 70μm and then vacuum degassed at 400°F to remove the hydrogen formed during electroplating. Laser surface alloying of chromium into the iron substrate was then performed employing a continuous wave CO2 laser operated at an incident power of 1200 watts. The laser beam, defocussed to a spot diameter of 0.25mm, scanned the material surface at a rate of 30mm/sec, (70 ipm).

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Electron microscopy of keratinocytes cultured on a collagen substrate used as an allograft for the treatment of chronic wounds

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100130158 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1104-1105

Author(s):

Debby A. Jennings ◽

Michael J. Morykwas ◽

Louis C. Argenta

Keyword(s):

Electron Microscopy ◽

Cell Suspension ◽

Single Cell ◽

Chronic Wounds ◽

Mechanical Stability ◽

Uv Light ◽

Type I ◽

Single Cell Suspension ◽

Collagen Substrate ◽

Dermal Collagen

Grafts of cultured allogenic or autogenic keratlnocytes have proven to be an effective treatment of chronic wounds and burns. This study utilized a collagen substrate for keratinocyte and fibroblast attachment. The substrate provided mechanical stability and augmented graft manipulation onto the wound bed. Graft integrity was confirmed by light and transmission electron microscopy.Bovine Type I dermal collagen sheets (100 μm thick) were crosslinked with 254 nm UV light (13.5 Joules/cm2) to improve mechanical properties and reduce degradation. A single cell suspension of third passage neonatal foreskin fibroblasts were plated onto the collagen. Five days later, a single cell suspension of first passage neonatal foreskin keratinocytes were plated on the opposite side of the collagen. The grafts were cultured for one month.The grafts were fixed in phosphate buffered 4% formaldehyde/1% glutaraldehyde for 24 hours. Graft pieces were then washed in 0.13 M phosphate buffer, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Polybed 812.

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Three-dimensional distribution of ribulose-1, 5-bisphosphate carboxylase/oxygemase during the early development of proplastids in dark-grown Euglena cells transferred to an inorganic medium

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162090 ◽

1990 ◽

Vol 48 (3) ◽

pp. 906-907

Author(s):

Tomoko Ehara ◽

Shuji Sumida ◽

Tetsuaki Osafune ◽

Eiji Hase

Keyword(s):

Cell Suspension ◽

Euglena Gracilis ◽

Carbon Materials ◽

Three Dimensional ◽

Peripheral Region ◽

Plastid Development ◽

Bisphosphate Carboxylase ◽

Inorganic Medium ◽

Ribulose 1 ◽

Dimensional Distribution

As shown previously, Euglena cells grown in Hutner’s medium in the dark without agitation accumulate wax as well as paramylum, and contain proplastids showing no internal structure except for a single prothylakoid existing close to the envelope. When the cells are transferred to an inorganic medium containing ammonium salt and the cell suspension is aerated in the dark, the wax was oxidatively metabolized, providing carbon materials and energy 23 for some dark processes of plastid development. Under these conditions, pyrenoid-like structures (called “pro-pyrenoids”) are formed at the sites adjacent to the prolamel larbodies (PLB) localized in the peripheral region of the proplastid. The single prothylakoid becomes paired with a newly formed prothylakoid, and a part of the paired prothylakoids is extended, with foldings, in to the “propyrenoid”. In this study, we observed a concentration of RuBisCO in the “propyrenoid” of Euglena gracilis strain Z using immunoelectron microscopy.

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