Viable but Nonculturable Cells in Plant-Associated Bacterial Populations

2000 ◽  
pp. 229-241 ◽  
Author(s):  
Mark Wilson ◽  
Steven E. Lindow
Keyword(s):  
Bacterial Populations ◽  
Viable But Nonculturable ◽  
Nonculturable Cells

SIGNIFICANCE OF VIABLE BUT NONCULTURABLE BACTERIA FOR SAFETY OF FOOD PRODUCTS

2019 ◽  
Vol 1 (2) ◽  
pp. 183-189
Author(s):  
A. M. Abdullaeva ◽  
◽  
L. P. Blinkova ◽  
Yu. D. Pakhomov ◽  
◽  
...  
Keyword(s):  
Food Products ◽  
Active State ◽  
Insufficient Data ◽  
Main Hypothesis ◽  
Viable But Nonculturable ◽  
Nonculturable Cells

In this review data on hazardous influence of nonculturable cells of pathogens on humans and animals, of contamination of foodstuffs is presented and also attention is stressed on properties of such cells and their effect through foodstuffs on humans and animals. Main hypothesis of formation and resuscitation of viable but nonculturable cells are elucidated. Factors that influence shifting bacteria to nonculturability and their conversion into active state are discussed. The conclusion is drawn about biohazard of viable nonculturable cells and insufficient data about their physiology and mechanisms of transition into this state and resuscitation back.

Formation of Viable but Nonculturable Cells

1993 ◽  
pp. 239-272 ◽  
Author(s):  
James D. Oliver
Keyword(s):  
Viable But Nonculturable ◽  
Nonculturable Cells

scholarly journals Respective Roles of Culturable and Viable-but-Nonculturable Cells in the Heterogeneity of Salmonella enterica Serovar Typhimurium Invasiveness

2009 ◽  
Vol 75 (16) ◽  
pp. 5179-5185 ◽  
Author(s):  
Julien Passerat ◽  
Patrice Got ◽  
Sam Dukan ◽  
Patrick Monfort
Keyword(s):  
Stationary Phase ◽  
Salmonella Enterica ◽  
Gradient Centrifugation ◽  
Health Concern ◽  
Viable But Nonculturable ◽  
Serovar Typhimurium ◽  
Nonculturable Cells ◽  
Sources Of Infection ◽  
Culturable Cell ◽  
Vbnc Cell

ABSTRACT The existence of Salmonella enterica serovar Typhimurium viable-but-nonculturable (VBNC) cells is a public health concern since they could constitute unrecognized sources of infection if they retain their pathogenicity. To date, many studies have addressed the ability of S. Typhimurium VBNC cells to remain infectious, but their conclusions are conflicting. An assumption could explain these conflicting results. It has been proposed that infectivity could be retained only temporarily after entry into the VBNC state and that most VBNC cells generated under intense stress could exceed the stage where they are still infectious. Using a Radioselectan density gradient centrifugation technique makes it possible to increase the VBNC-cell/culturable-cell ratio without increasing the exposure to stress and, consequently, to work with a larger proportion of newly VBNC cells. Here, we observed that (i) in the stationary phase, the S. Typhimurium population comprised three distinct subpopulations at 10, 24, or 48 h of culture; (ii) the VBNC cells were detected at 24 and 48 h; (iii) measurement of invasion gene (hilA, invF, and orgA) expression demonstrated that cells are highly heterogeneous within a culturable population; and (iv) invasion assays of HeLa cells showed that culturable cells from the different subpopulations do not display the same invasiveness. The results also suggest that newly formed VBNC cells are either weakly able or not able to successfully initiate epithelial cell invasion. Finally, we propose that at entry into the stationary phase, invasiveness may be one way for populations of S. Typhimurium to escape stochastic alteration leading to cell death.

Injured microorganisms and viable but nonculturable cells

2022 ◽  
pp. 65-71
Author(s):  
Osman Erkmen
Keyword(s):  
Viable But Nonculturable ◽  
Nonculturable Cells

scholarly journals Induction of Escherichia coli and Salmonella typhimurium into the viable but nonculturable state following chlorination of wastewater

2005 ◽  
Vol 3 (3) ◽  
pp. 249-257 ◽  
Author(s):  
James D. Oliver ◽  
Maya Dagher ◽  
Karl Linden
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Stationary Phases ◽  
Health Hazard ◽  
Physiological State ◽  
Treated Wastewater ◽  
Vbnc State ◽  
Viable But Nonculturable ◽  
Nonculturable Cells ◽  
Nonculturable State

We examined the effects of chlorine disinfection on Escherichia coli and Salmonella typhimurium in secondary-treated wastewater to determine whether such treatment might induce these bacteria into the viable but nonculturable (VBNC) state. In this state, cells lose culturability but retain viability and the potential to revert to the metabolically active and infectious state. To examine the effects of chlorination on cells in different physiological states, cells from the logarithmic and stationary phases, or nutrient starved, or grown in natural wastewater, were studied. Isogenic cells with and without plasmids were also examined. Whereas a mixture of free and combined chlorine, as occurs under typical wastewater disinfection, was found to be rapidly lethal to most cells, regardless of their physiological state or plasmid content, c. 104 of the original 106 cells ml−1 did survive in the VBNC state. While we were not successful in resuscitating these cells to the culturable state, the presence of such nonculturable cells in treated wastewater offers a potential public health hazard.

scholarly journals Resuscitation of viable but nonculturable cells ofVibrio parahaemolyticusinduced at low temperature under starvation

2000 ◽  
Vol 186 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Yoshimitsu Mizunoe ◽  
Sun Nyunt Wai ◽  
Takahiko Ishikawa ◽  
Akemi Takade ◽  
Shin-ichi Yoshida
Keyword(s):  
Low Temperature ◽  
Viable But Nonculturable ◽  
Nonculturable Cells

ANALYSIS OF THE EFFECT OF PHAGES ON BACTERIA AND THEIR VIABLE BUT NONCULTURABLE CELLS

2021 ◽  
Vol 1 (3) ◽  
pp. 317-322
Author(s):  
T.A. Karachina ◽  
◽  
A.M. Abdullaeva ◽  
L.P. Blinkova ◽  
Yu.D. Pakhomov ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Raw Materials ◽  
Animal Origin ◽  
Meat Processing ◽  
Viable But Nonculturable ◽  
Chemical Substances ◽  
E Coli ◽  
Processing Plants ◽  
Nonculturable Cells ◽  
Alternative Means

The growth in the use of antibiotic and chemical substances in meat processing plants shows the need to search for alternative means of decontamination, the most promising of which are bacteriophages. However, there are a lot of little-studied questions on their use, one of which is the effect of bacteriophages on viable but nonculturable (VBNC) cells of bacteria. In this regard, the article provides data on the analysis of the activity of commercial bacteriophages in relation to bacteriacontaminants of raw materials of animal origin, in particular E. coli and Salmonella enterica typhimurium, as well as their VBNC-cells.

In planta recovery of Erwinia amylovora viable but nonculturable cells

Trees ◽  
2011 ◽  
Vol 26 (1) ◽  
pp. 75-82 ◽  
Author(s):  
R. D. Santander ◽  
J. F. Català-Senent ◽  
E. Marco-Noales ◽  
E. G. Biosca
Keyword(s):  
Erwinia Amylovora ◽  
In Planta ◽  
Viable But Nonculturable ◽  
Nonculturable Cells
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