Polysialic Acids: Potential for Long Circulating Drug, Protein, Liposome and Other Microparticle Constructs

1998 ◽  
pp. 193-205
Author(s):  
Gregory Gregoriadis ◽  
Ana Fernandes ◽  
Brenda McCormack ◽  
Malini Mital ◽  
Xiaoqin Zhang
Keyword(s):  
Polysialic Acids

scholarly journals Chemical Polysialylation and In Vivo Tetramerization Improve Pharmacokinetic Characteristics of Recombinant Human Butyrylcholinesterase-Based Bioscavengers

Acta Naturae ◽  
2015 ◽  
Vol 7 (4) ◽  
pp. 136-141 ◽  
Author(s):  
S. S. Terekhov ◽  
I. V. Smirnov ◽  
O. G. Shamborant ◽  
T. V. Bobik ◽  
D. G. Ilyushin ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Large Scale ◽  
Promising Candidate ◽  
Protective Index ◽  
Polysialic Acids ◽  
Long Acting ◽  
Nerve System ◽  
Molecular Compounds ◽  
Human Butyrylcholinesterase

Organophosphate toxins (OPs) are the most toxic low-molecular compounds. The extremely potent toxicity of OPs is determined by their specificity toward the nerve system. Human butyrylcholinesterase (hBChE) is a natural bioscavenger against a broad spectrum of OPs, which makes it a promising candidate for the development of DNA-encoded bioscavengers. The high values of the protective index observed for recombinant hBChE (rhBChE) make it appropriate for therapy against OP poisoning, especially in the case of highly toxic warfare nerve agents. Nevertheless, large-scale application of biopharmaceuticals based on hBChE is restricted due to its high cost and extremely rapid elimination from the bloodstream. In the present study, we examine two approaches for long-acting rhBChE production: I) chemical polysialylation and II) in-vivo tetramerization. We demonstrate that both approaches significantly improve the pharmacokinetic characteristics of rhBChE (more than 5 and 10 times, respectively), which makes it possible to use rhBChE conjugated with polysialic acids (rhBChE-CAO) and tetrameric rhBChE (4rhBChE) in the treatment of OP poisonings.

[27] Identification of polysialic acids in glycoconjugates

1994 ◽  
pp. 460-484 ◽  
Author(s):  
Jean Ye ◽  
Ken Kitajimaya ◽  
Yasuo Inoue ◽  
Sadako Inoue ◽  
Frederic A. Troy
Keyword(s):  
Polysialic Acids

Biosynthesis and production of polysialic acids in bacteria

2010 ◽  
Vol 86 (6) ◽  
pp. 1621-1635 ◽  
Author(s):  
Miguel Ángel Ferrero ◽  
Leandro Rodríguez Aparicio
Keyword(s):  
Polysialic Acids
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