Regulation of Expression of Taurine Transport in Two Continuous Renal Epithelial Cell Lines and Inhibition of Taurine Transporter by a Site-Directed Antibody

1996 ◽  
pp. 173-191 ◽  
Author(s):  
Xiaobin Han ◽  
Russell W. Chesney ◽  
Andrea M. Budreau ◽  
Deborah P. Jones
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Taurine Transporter ◽  
Regulation Of Expression ◽  
Renal Epithelial Cell ◽  
Taurine Transport ◽  
Epithelial Cell Lines ◽  
A Site

scholarly journals Adaptive regulation of taurine transport in two continuous renal epithelial cell lines

1990 ◽  
Vol 38 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Deborah P. Jones ◽  
Leslie A. Miller ◽  
Russell W. Chesney
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Renal Epithelial Cell ◽  
Taurine Transport ◽  
Adaptive Regulation ◽  
Epithelial Cell Lines

Deficiency in intercellular communication in two established renal epithelial cell lines (LLC-PK1 and MDCK)

1984 ◽  
Vol 66 (1) ◽  
pp. 81-93
Author(s):  
F.V. Sepulveda ◽  
J.D. Pearson
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Mdck Cells ◽  
Cell Communication ◽  
Intercellular Junctions ◽  
Cell Types ◽  
Renal Epithelial Cell ◽  
Aortic Endothelial Cells ◽  
Epithelial Cell Lines ◽  
Autoradiographic Analysis

We have studied the cell-to-cell passage of uridine nucleotides in two renal epithelial cell lines (LLC-PK1 and MDCK) and in porcine aortic endothelial cells (PAE). All three cell types incorporated tritiated uridine. After a 3 h incubation the radioactivity was predominantly in the form of acid-soluble compounds, mainly UTP. Prelabelled LLC-PK1 or MDCK cells were unable to transfer radioactivity to added adjacent, non-labelled cells, whereas PAE cells readily formed communicating intercellular junctions, as judged by autoradiographic analysis after a 3 h co-culture period. Cell-to-cell communication in either of the renal cell lines was not promoted by treatment with dibutyryl cyclic AMP and methylisobutylxanthine. Radioactivity incorporated into the acid-insoluble pool was not available for intercellular transfer, as assessed in experiments in which cells were prelabelled 24 h before co-culture.

Renal epithelial cell lines (BSC-1, MDCK, LLC-PK1) express 11β-hydroxysteroid dehydrogenase activity

1989 ◽  
Vol 1010 (3) ◽  
pp. 311-317 ◽  
Author(s):  
Christoph Korbmacher ◽  
Wolfgang Schulz ◽  
Michael König ◽  
Harald Siebe ◽  
Ingrid Lichtenstein ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Dehydrogenase Activity ◽  
Cell Lines ◽  
Hydroxysteroid Dehydrogenase ◽  
Renal Epithelial Cell ◽  
Epithelial Cell Lines

Polarity of taurine transport in cultured renal epithelial cell lines: LLC-PK1 and MDCK

1993 ◽  
Vol 265 (1) ◽  
pp. F137-F145 ◽  
Author(s):  
D. P. Jones ◽  
L. A. Miller ◽  
R. W. Chesney
Keyword(s):  
Amino Acid ◽  
Epithelial Cell ◽  
Mdck Cell ◽  
Amino Acid Transport ◽  
Acid Transport ◽  
Renal Epithelial Cell ◽  
Amino Acid Transport System ◽  
Taurine Transport ◽  
Taurine Concentration ◽  
Epithelial Cell Lines

We characterized taurine transport in two continuous renal epithelial cell lines: LLC-PK1, derived from the proximal tubule of the pig, and the Madin-Darby canine kidney (MDCK), which was originated from the distal tubule of the dog. In the LLC-PK1 cell line, taurine transport is greatest at the apical surface of the cell, whereas in the MDCK cell line taurine transport is greatest at the basolateral surface. Both apical and basolateral surfaces of LLC-PK1 and MDCK cells exhibit an adaptive response to the extracellular taurine concentration (medium taurine concentration). Only the basolateral surface of the MDCK cell responded to hyperosmolality with increased taurine accumulation. This indicates differential control of the beta-amino acid transport system by substrate and external tonicity. The function of the beta-amino acid transport system may be different depending on the cell. In the LLC-PK1 cell, there is net transepithelial movement of taurine and changes in transporter activity in response to supply of substrate. In contrast, taurine accumulation by the MDCK cell appears to be a mechanism for adaptation to osmotic stress.

scholarly journals CD14- and Toll-Like Receptor-Dependent Activation of Bladder Epithelial Cells by Lipopolysaccharide and Type 1 Piliated Escherichia coli

2003 ◽  
Vol 71 (3) ◽  
pp. 1470-1480 ◽  
Author(s):  
Joel D. Schilling ◽  
Steven M. Martin ◽  
David A. Hunstad ◽  
Kunal P. Patel ◽  
Matthew A. Mulvey ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Lines ◽  
Epithelial Cell Line ◽  
Renal Epithelial Cell ◽  
E Coli ◽  
Epithelial Cell Lines ◽  
Renal Epithelial Cell Line

ABSTRACT The gram-negative bacterium Escherichia coli is the leading cause of urinary tract infection. The interaction between type 1 piliated E. coli and bladder epithelial cells leads to the rapid production of inflammatory mediators, such as interleukin-6 (IL-6) and IL-8. Conflicting reports have been published in the literature regarding the mechanism by which uroepithelial cells are activated by type 1 piliated E. coli. In particular, the role of lipopolysaccharide (LPS) in these responses has been an area of significant debate. Much of the data arguing against LPS-mediated activation of bladder epithelial cells have come from studies using a renal epithelial cell line as an in vitro model of the urinary epithelium. In this report, we analyzed three bladder epithelial cell lines and demonstrated that they all respond to LPS. Furthermore, the LPS responsivity of the cell lines directly correlated with their ability to generate IL-6 after E. coli stimulation. The LPS receptor complex utilized by the bladder epithelial cell lines included CD14 and Toll-like receptors, and signaling involved the activation of NF-κB and p38 mitogen-activated protein kinase. Also, reverse transcription-PCR analysis demonstrated that bladder epithelial cells express CD14 mRNA. Thus, the molecular machinery utilized by bladder epithelial cells for the recognition of E. coli is very similar to that described for traditional innate immune cells, such as macrophages. In contrast, the A498 renal epithelial cell line did not express CD14, was hyporesponsive to LPS stimulation, and demonstrated poor IL-6 responses to E. coli.

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